We narrowed to 10,009 results for: ADA
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Plasmid#86234PurposeFor transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locusDepositorInsertspCas9-nickase and sgRNA against mouse rosa26 locus
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMK312 (TOP2A CRISPR)
Plasmid#140654PurposeTOP2A tagging CRISPRDepositorAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-mTrim21ΔRING-Box
Plasmid#105517PurposeExpression of mCherry-tagged mouse Trim21 with a deleted RING-box in mammalian cellsDepositorInsertTRIM21 (Trim21 Mouse)
UseTagsmCherryExpressionMammalianMutationdeletion of RING domain (aa124-462)PromoterCMVAvailable SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRvSAHS1-SAHS1-mEGFP
Plasmid#205007PurposeThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the RvSAHS1-mEGFP gene positioned in between.DepositorInsertRvSAHS1-mEGFP with 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvTUB-mEGFP-NLS
Plasmid#205011PurposeThe vector contains 1kbp of the upstream and downstream regions of the tubulin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the tubulin gene from R. varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-mTrim21ΔPRYSPRY
Plasmid#105518PurposeExpression of mCherry-tagged mouse Trim21 with a deleted PRYSPRY domain in mammalian cellsDepositorInsertTRIM21 (Trim21 Mouse)
UseTagsmCherryExpressionMammalianMutationdeletion of PRYSPRY domain (aa1-281)PromoterCMVAvailable SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)
Plasmid#71719PurposeExpressing a GFP-tagged degron in the soma of C. elegansDepositorInsertauxin-responsive protein IAA17 (AXR3 Mustard Weed)
UseTagsEmGFPExpressionWormMutationminimal degron sequence (71-114aa) with start cod…Promotereef-1A.1 (eft-3)Available SinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
tUI
Plasmid#122661PurposeExpress tUI protein in E. coliDepositorAvailable SinceJuly 17, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
XE251 pCDNA3.1(zeo)-hDsh3
Plasmid#16758DepositorAvailable SinceMarch 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FAS-GCaMP6f-WPRE
Plasmid#216277PurposeAAV vector with hSynapsin promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in neurons that express Cre recombinase)DepositorInsertGCaMP6f
UseAAV and Cre/LoxTags6XHIS and XpressExpressionMutationPromoterhuman synapsin 1 (hSyn)Available SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA
Plasmid#117687PurposeU6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.DepositorInsertsKanamycin cassette
TetR-P2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and hPGKAvailable SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-His-TEV-NQO1
Plasmid#119058PurposeFor expression in E.coli and affinity purification of N-terminally His tagged NQO1DepositorInsertNAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
UseTags6His, TEV siteExpressionBacterialMutationPromoterT7Available SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPS
Plasmid#85801PurposeBackbone for pooled cloning of Perturb-seq librariesDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available SinceMarch 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET42b-ABE7.10
Plasmid#120398PurposeExpresses ABE7.10-NLS with an N-terminal His Tag (His6) for bacterial expressionDepositorInsertABE7.10
UseTagsHisExpressionBacterialMutationMammalian codon-optimizedPromoterT7Available SinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRvSAHS1-mEGFP-NLS
Plasmid#205005PurposeThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvCAHS3-mEGFP-NLS
Plasmid#205004PurposeThe vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA SF-HERC2 F4
Plasmid#55787PurposeExpresses N-terminal 3xFLAG/STREP tagged HERC2(2600-3600aa)DepositorInsert3xFLAG tagged HERC2 (2600-3600aa) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationPromoterCMVAvailable SinceJuly 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/er-(n2)oxStayGold(c4)
Plasmid#185822PurposeA soluble photostable fluorescent marker of the ER lumen. The StayGold gene has no codon optimization for mammalian expression.DepositorInserter-(n2)oxStayGold(c4)
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMK395 (AAVS1 CMV-OsTIR1(F74A))
Plasmid#159992PurposeExpression of OsTIR1(F74A) in human cellsDepositorInsertCMV-OsTIR1(F74A)
UseTagsExpressionMammalianMutationPromoterAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV10.F3
Plasmid#63199PurposeBioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable SinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only