We narrowed to 10,133 results for: ada

1. pX330-PITCh-TUBA1B

   Plasmid

   #207763

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of TUBA1B (TUBA1B Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT3TS-spCas9 ABE-Umax-ex2-rest1

   Plasmid

   #222143

   Purpose

   Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   Modified TadA-nSpCas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Bipartite NLS

   Promoter

   T3

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHeEf1a-mEGFP-NLS

   Plasmid

   #205012

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the ef1a gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mEGFP-NLS flanked by 1kbp upstream and downstream of the ef1a gene from Hypsibius exemplaris
   

   Use

   Tardigrade expression

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSET-6xTR-TUBE

   Plasmid

   #110313

   Purpose

   E. coli expression and purification of 6xTR-TUBE

   Depositor

   Yasushi Saeki

   Article

   Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

   Insert

   6xTR-TUBE (UBQLN1 Human)
   

   Tags

   N-terminal His6-T7 tag

   Expression

   Bacterial

   Mutation

   All Arg residues in the UBA domain are mutated to…

   Promoter

   T7

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. xCas9(3.7)-ABE(7.10)

   Plasmid

   #108382

   Purpose

   Mammalian expression vector for xCas9(3.7)-ABE

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9(3.7)-ABE(7.10)
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 HA Cyclin F

   Plasmid

   #236466

   Purpose

   transient overexpression of Cyclin F in mammalian cells

   Depositor

   Michele Pagano

   Article

   D'Angiolella et al Cell. 2012 May 25;149(5):1023-34. doi: 1

   Insert

   Cyclin F (CCNF Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA4-NHK-MYC-His6

   Plasmid

   #185350

   Purpose

   Assessing of ubiquitination in cells

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   a1-antitrypsin Hong Kong Null mutant
   

   Tags

   Myc, 6xHis

   Expression

   Mammalian

   Mutation

   NHK mutant

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-CETN2

   Plasmid

   #227291

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of CETN2 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of CETN2 (CETN2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. MAC_N_KLF9

   Plasmid

   #167774

   Purpose

   Expresses KLF9 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   KLF9 (KLF9 Human)
   

   Tags

   MAC

   Expression

   Mammalian

   Mutation

   no mutations

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT3TS-spCas9 ABE-Umax-ex1

    Plasmid

    #222141

    Purpose

    Adenine base editor with modified TadA variant for extended window for A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Promoter

    T3

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. ACBE-NW

    Plasmid

    #240624

    Purpose

    A-to-C base editor with narrowed editing window

    Depositor

    Tingting Jiang

    Article

    Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:

    Insert

    TadA8e(N108Q, M151C, Q154Y)-nCas9 (D10A)-mAAG
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    TadA8e(N108Q, M151C, Q154Y),Cas9 (D10A),mAAG (R16…

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT3TS-spCas9 ABE-Umax-ex1-rest1

    Plasmid

    #222140

    Purpose

    Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Promoter

    T3

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti Dest BlastR R1-R2

    Plasmid

    #84574

    Purpose

    Gateway destination vector expressing Blasticidin resistance under a PGK promoter

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Type

    Empty backbone

    Use

    Lentiviral; Gateway

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRX-IP-EGFP-mouse Plaat3 (N157stop)

    Plasmid

    #168771

    Purpose

    Expresses EGFP-Plaat3 (C-terminal element is deleted) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

    Insert

    Hrasls3 (Plaat3 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEMHE-mEGFP-Map4

    Plasmid

    #105571

    Purpose

    T7 promotor drives in vitro transcription of mEGFP-tagged Histon MAP4 mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    MAP4 (Map4 Mouse)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Promoter

    T7

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1-Tet-OsTIR1(WT)-V5

    Plasmid

    #158663

    Purpose

    Tet-OsTIR1(WT)-V5 donor for integration at the AAVS1 locus

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    Tet-OsTIR1(WT) AAVS1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUltra-sY

    Plasmid

    #82417

    Purpose

    Expresses Mj aaRS for sulfotyrosine and Mj tRNA for decoding UAG codons

    Depositor

    Chang Liu

    Article

    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

    Insert

    Methanococcus jannaschii aaRS for sulfotyrosine
    

    Expression

    Bacterial

    Mutation

    Tyr32Leu, Leu65Pro, Asp158Gly, Ile159Cys, Leu162L…

    Promoter

    tacI

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. retro-gRNA-eGFP

    Plasmid

    #116926

    Purpose

    Retrovirus expressing eGFP with BBSI cloning sites for sgRNA

    Depositor

    Christophe Benoist, Diane Mathis

    Article

    Magnuson et al Proc Natl Acad Sci U S A. 2018 Nov 6;115

    Insert

    eGFP
    

    Use

    Retroviral

    Promoter

    EF1a

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGPTVII-SED1

    Plasmid

    #232615

    Purpose

    SED1 biosensor for expression in Arabidopsis thaliana

    Depositor

    Jose Dinneny

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 Biosensor
    

    Expression

    Plant

    Promoter

    pUBQ10

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS-PKCdelta-EGFP

    Plasmid

    #178856

    Purpose

    Mammalian expression of PKCδ fused to EGFP

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    PKCδ (PRKCD Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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