We narrowed to 7,293 results for: 11

1. pTYF-PRSx8-AstR-p2A-GFP

   Plasmid

   #159632

   Purpose

   Lentiviral expression of the Allatostatin receptor, P2A GFP under the PRSx8 promoter

   Depositor

   Andrew Allen

   Article

   Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

   Insert

   AstR (AstA-R1 Fly)
   

   Use

   Lentiviral

   Tags

   GFP and P2A

   Expression

   Mammalian

   Promoter

   PRSx8

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Venus-BimEL-pEGFP-C1

   Plasmid

   #166735

   Purpose

   Express Venus fused to the N-terminus of the Bcl-2 family protein, BimEL

   Depositor

   David Andrews

   Article

   Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

   Insert

   BimEL (Bcl2l11 Mouse)
   

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. shFGF2#2

   Plasmid

   #157668

   Purpose

   Tet inducible knockdown of FGF2

   Depositor

   Byung Il Lee

   Article

   Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

   Insert

   Fibroblast growth factor 2 (FGF2 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB3051(gRNA X-3 XI-2 XII-2)

   Plasmid

   #73293

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag-M4-AR-W741C

   Plasmid

   #171247

   Purpose

   Mammalian expression of 3xFlag-tagged human androgen receptor mutant that binds to bicalutamide: AR-Trp741Cys (AR-W741C)

   Depositor

   Steven Balk

   Article

   Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

   Insert

   3xFlag-tagged human androgen receptor Trp741Cys mutant (AR Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   AR-Trp741Cys (AR-W741C)

   Promoter

   CMV

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-EphB4-Fc

   Plasmid

   #200986

   Purpose

   Mammalian expression plasmid for soluble EphB4 fused to IgG1 Fc

   Depositor

   Erik Procko

   Article

   Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

   Insert

   EphB4 (EPHB4 Human)
   

   Tags

   Fc region of human IgG1

   Expression

   Mammalian

   Mutation

   Soluble ectodomain of EphB4

   Promoter

   CMV

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1

   Plasmid

   #10880

   Depositor

   Patrick Aebischer

   Article

   Raoul et al Nat Med. 2005 Apr . 11(4):423-8.

   Insert

   H1-shSOD1 (SOD1 Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

8. UbG76V-eGFP-V5His_15aa

   Plasmid

   #23969

   Depositor

   Nico Dantuma

   Article

   Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

   Insert

   Ub-G76V-eGFP-V5HIS_15aa (UBB Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   G76V mutation in Ubiquitin. Ub fused to N-termin…

   Available Since

   April 16, 2010

   Availability

   Academic Institutions and Nonprofits only

9. Mutant Human Cortactin-GFP

   Plasmid

   #71365

   Purpose

   mammalian expression of mutant human Cortactin-GFP

   Depositor

   Alpha Yap

   Article

   Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

   Insert

   cortactin (CTTN Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   silent mutation present at 72nd nucleotide

   Promoter

   CMV

   Available Since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pSICO-CPSF6-358-iRFP670

    Plasmid

    #110694

    Purpose

    Expresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with iRFP670

    Depositor

    Zandrea Ambrose

    Article

    Ning et al J Virol. 2018 Apr 11. pii: JVI.00368-18.

    Insert

    CPSF6-iRFP670 (CPSF6 Human)
    

    Use

    Lentiviral

    Tags

    iRFP670

    Mutation

    Truncation of CPSF6 isoform at amino acid 358

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS313_ATP3

    Plasmid

    #120253

    Purpose

    Expression of ATP3 in yeast

    Depositor

    Markus Ralser

    Article

    Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

    Insert

    ATP3 (ATP3 Budding Yeast)
    

    Expression

    Yeast

    Promoter

    ATP3

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. VRQR-HF1 variant

    Plasmid

    #138563

    Purpose

    Expresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VRQR-HF1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. JPF0419d

    Plasmid

    #124020

    Purpose

    Encodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCAG-Lyn-iRFP713-P2A-NES-mAzamiGreen-P2A-p38KTR-mCerulean-P2A-H2B::mScarlet
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. CDK2-3XFlag-pBabe

    Plasmid

    #73557

    Purpose

    vector encoding a fusion protein between CDK2 and 3 Flag tags

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    CDK2 (CDK2 Human)
    

    Use

    Retroviral

    Tags

    3X-FLAG

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

15. pNCST-AausFP2

    Plasmid

    #129500

    Purpose

    Expresses AausFP2 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AausFP2
    

    Expression

    Bacterial

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. STARR-seq luciferase validation vector_SCP1_SV40

    Plasmid

    #99311

    Purpose

    Luciferase validation vector with SV40 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Insert

    SV40 enhancer
    

    Use

    Luciferase; Starr-seq luciferase validation vector

    Expression

    Mammalian

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-N1 VAMP2 R125TAG

    Plasmid

    #69876

    Purpose

    express in mammalian cells for unnatural amino acid incorporation into VAMP2-GFP

    Depositor

    Silvio Rizzoli

    Article

    Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

    Insert

    VAMP2-GFP (Vamp2 Rat)
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Amber stop codon in the linker between VAMP2 and …

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pNCST-AausGFP

    Plasmid

    #129503

    Purpose

    Expresses AausGFP constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AausGFP
    

    Expression

    Bacterial

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag-M4-AR-S81D

    Plasmid

    #171242

    Purpose

    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser81Asp (AR-S81D)

    Depositor

    Steven Balk

    Article

    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

    Insert

    3xFlag-tagged human androgen receptor Ser81Asp mutant (AR Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-Ser81Asp (AR-S81D)

    Promoter

    CMV

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-Hygro-sgSIK3

    Plasmid

    #138699

    Purpose

    Expresses a human SIK3-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK3 human (SIK3 Human)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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