We narrowed to 14,045 results for: crispr grnas

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pDest_U6_A2_CG2_T3_gRNA

Plasmid

#199389

Purpose

pDEL113; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type III sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

nrg1 type III sgRNA

Use

Tol2 destination vector

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pDest_U6_A2_CG2_typeEGF_gRNA

Plasmid

#199386

Purpose

pDEL110; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 EGF sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

nrg1 EGF sgRNA

Use

Tol2 destination vector

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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LentiU6-hASCL1 gRNA_1-MS2-Puro

Plasmid

#192670

Purpose

Lentiviral expression of sgRNA targeting hASCL1 promoter to activate human ASCL1 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human ASCL1 activating gRNA #1 (ASCL1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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LentiU6-hASCL1 gRNA_multi1-3-MS2-Puro

Plasmid

#192682

Purpose

Lentiviral expression of multi gRNAs targeting hASCL1 promoter to activate human ASCL1 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human ASCL1 activating gRNAs #1,2,3 (ASCL1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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LentiU6-hIL1RN gRNA_1-MS2-Puro

Plasmid

#192677

Purpose

Lentiviral expression of sgRNA targeting hIL1RN promoter to activate human IL1RN transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human IL1RN activating gRNA #1 (IL1RN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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peSpCas9(1.1)-2×sgRNA (IFT88, donor)

Plasmid

#80769

Purpose

Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Insert

IFT88 gRNA#1 (IFT88 Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)

Plasmid

#71829

Purpose

Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control

Depositor

Vlatka Zoldos

Article

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

BACH2-sgRNA8 (BACH2 Human, Synthetic, S. pyogenes)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-PuroR

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9; E756A inactiva…

Promoter

U6

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

Plasmid

#67986

Purpose

Cas9 activity reporter with mCherry and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Inserts

U6gRNA cassette, PGKmCherryABFP cassette, WPRE
Guide RNA targeting modified BFP

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W

Plasmid

#67974

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2ABFP cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pDY0663 ACTB atgRNA with v2 scaffold

Plasmid

#179108

Purpose

ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffold

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

ACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold

Use

CRISPR

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP

Plasmid

#67991

Purpose

CRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPRE

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette with an improved scaffold, PGKpuro2ABFP cassette

Use

CRISPR and Lentiviral

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W

Plasmid

#67984

Purpose

Cas9 activity reporter with GFP and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Inserts

U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
Guide RNA targeting modified BFP

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFP

Plasmid

#67990

Purpose

CRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPRE

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette with the conventional scaffold, PGKpuro2ABFP cassette

Use

CRISPR and Lentiviral

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W

Plasmid

#67983

Purpose

Cas9 activity reporter (control) with GFP and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKGFP2ABFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pBluescriptSKII+ U6-sgRNA(F+E) empty

Plasmid

#74707

Purpose

Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence

Expression

Mammalian

Available Since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-SCR-sgRNA-A

Plasmid

#177811

Purpose

Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

Scrambled sgRNA targeting sequences

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-SCR-sgRNA-B

Plasmid

#177812

Purpose

Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

Scrambled sgRNA targeting sequences

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-Dvl1/3 gRNA

Plasmid

#246555

Purpose

CRISPR vector co-expressing Cas9 and a mouse Dvl1/3 gRNA (conserved region)

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Dvl1/3 gRNA

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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MiniCoopR U6:gRNA, mitfa:Cas9

Plasmid

#118840

Purpose

Targets a gene and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Type

Empty backbone

Use

CRISPR; Tol2

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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Human SOX17 sgRNA

Plasmid

#59725

Purpose

Plasmid encoding sgRNA to generate SOX17 knock-out mutant human cells

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

hSox17 sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only