We narrowed to 17,570 results for: bon;

1. pLVNP2.0-SpCas9

   Plasmid

   #206880

   Purpose

   Expresses FLAG-tagged SpCas9 fused to the N-terminus of Gag/GagPol harbouring an intervening phospholipase C-δ1 pleckstrin homology (PH) domain

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

   Insert

   SpCas9 (cas9 )
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Promoter

   CMV

   Available Since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223_NMNAT3_WT

   Plasmid

   #81885

   Purpose

   Gateway Donor vector containing NMNAT3 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   NMNAT3 (NMNAT3 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. LCV2_mCherry

   Plasmid

   #155096

   Purpose

   Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mCherry expression

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. LCV2_mClover3

   Plasmid

   #155095

   Purpose

   Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mClover3 expression

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-UCOE-SFFV-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

   Plasmid

   #188765

   Purpose

   dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   dCas9-XTEN80-KRAB(Kox1)
   

   Use

   Lentiviral

   Tags

   HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

   Promoter

   SFFV

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSCAR_sgRNA_puro-mKate-lox5171

   Plasmid

   #162077

   Purpose

   3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1+FLAG-KrasnatG12D

   Plasmid

   #206842

   Purpose

   To express mouse Kras encoded by native mouse codons and with a G12D mutation with an N-terminal FLAG tag.

   Depositor

   Christopher Counter

   Article

   Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

   Insert

   Kras (Kras Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Glycine 12 to Aspartic Acid

   Available Since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pscALPSblasti-TMPRSS2 Blasti

   Plasmid

   #158088

   Purpose

   Expresses TMPRSS2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   TMPRSS2 (TMPRSS2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGAPTrap-mtagBFP2-IRESMuro

   Plasmid

   #82335

   Purpose

   Vector targeting the mtagBFP2 gene to the GAPDH locus of human cells. BFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

   Depositor

   Ed Stanley

   Article

   Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

   Insert

   mtagBFP2-IRESMuro
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223_TRIM55_WT

    Plasmid

    #81829

    Purpose

    Gateway Donor vector containing TRIM55 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    TRIM55 (TRIM55 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR_UCOE-EF1a-dCas9-XTEN80-KRAB(Kox1)-IRES-mCherry

    Plasmid

    #188769

    Purpose

    dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    dCas9-XTEN80-KRAB(Kox1)
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Promoter

    EF1a

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFB1.HMBP.PrS.JARID2

    Plasmid

    #125166

    Purpose

    Expresses human JARID2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    JARID2 (JARID2 Human)
    

    Tags

    His-MBP

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223_PRKACG_WT

    Plasmid

    #82156

    Purpose

    Gateway Donor vector containing PRKACG , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PRKACG (PRKACG Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHR-UCOE-CMV-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

    Plasmid

    #188772

    Purpose

    dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    dCas9-XTEN80-KRAB(Kox1)
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Promoter

    CMV

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCOLADuet-1_nsp10 (SARS-CoV-2)

    Plasmid

    #169158

    Purpose

    To express SARS-CoV-2 nsp10 in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    nsp10 (ORF1ab SARS-CoV-2)
    

    Tags

    Methionine

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS D614 soluble Spike

    Plasmid

    #164652

    Purpose

    Expresses SARS-CoV-2 soluble Spike protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 soluble spike (S SARS-CoV-2)
    

    Tags

    HIS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

17. pDONR223_WNK3_p.V488A

    Plasmid

    #81723

    Purpose

    Gateway Donor vector containing WNK3, part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    WNK3 (WNK3 Human)
    

    Use

    Gateway entry vector

    Mutation

    V488A

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223_POU2F2_WT

    Plasmid

    #81873

    Purpose

    Gateway Donor vector containing POU2F2 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    POU2F2 (POU2F2 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCK005_U6-Sp-sgRNA_UP-TdTomato_WPRE

    Plasmid

    #85453

    Purpose

    Lentiviral CRISPR system backbone bearing BsmBI site for S. pyogenes new guide RNAs and TdTomato.

    Depositor

    Aviv Regev

    Article

    Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

    Insert

    U6-sgRNA-Backbone-UP-TdTomato
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    deletion of AA123-365 in tdTomato (please see dep…

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223_PPP6C_WT

    Plasmid

    #81811

    Purpose

    Gateway Donor vector containing PPP6C , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PPP6C (PPP6C Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge