We narrowed to 14,602 results for: pET

1. PIA900

   Plasmid

   #104401

   Purpose

   Polycistronic vector for expression of E. coli core RNAP

   Depositor

   Irina Artsimovitch

   Article

   Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

   Insert

   rpoA, B, C, and Z
   

   Use

   E. coli expression vector

   Tags

   encodes a C-terminally His-tagged rpoC which can …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCri-1b

   Plasmid

   #61327

   Purpose

   Protein expression.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

   Insert

   Green fluorescent protein
   

   Use

   Tags

   His-tag and MBP-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pNIC-ZB

   Plasmid

   #26107

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Jun 10. ():.

   Type

   Empty backbone

   Use

   Tags

   His6 - Z-basic - TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKEW397_AcrIIA15

   Plasmid

   #138285

   Purpose

   Expression of AcrIIA15 protein

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   AcrIIA15
   

   Use

   CRISPR

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKEW394_AcrIIA14

   Plasmid

   #138283

   Purpose

   Expression of AcrIIA14 protein

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   AcrIIA14
   

   Use

   CRISPR

   Tags

   SFGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAM282

   Plasmid

   #226121

   Purpose

   Expression of cdc48

   Depositor

   Andreas Martin

   Article

   Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

   Insert

   His6-3C-Cdc48 (CDC48 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTSA-C

   Plasmid

   #17329

   Depositor

   Takeshi Sano

   Article

   Reznik et al Bioconjug Chem. . 12(6):1000-4.

   Insert

   streptavidin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Truncated to include only amino acids 16-133 of m…

   Promoter

   Available since

   Feb. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pLysscys255

   Plasmid

   #36429

   Depositor

   Emine Gunhan, Pete Lollar

   Article

   Gunhan et al Protein Expr Purif. 2008 Apr;58(2):203-9

   Insert

   Saporin-3/cys255
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   cys added at position 255

   Promoter

   T7

   Available since

   May 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. KRigor980

   Plasmid

   #129763

   Purpose

   Expresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfaces

   Depositor

   William Hancock

   Insert

   KHC (Khc Fly)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   R210A

   Promoter

   Available since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBD-0001

    Plasmid

    #12373

    Depositor

    Axel Brunger

    Article

    DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

    Insert

    VCP (Vcp Mouse)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. K560_AviC_coBirA

    Plasmid

    #129765

    Purpose

    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assays

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Use

    Tags

    Avi-tag GLNDIFEAQKIEWH and His6

    Expression

    Bacterial

    Mutation

    Truncation at 559

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUG-124

    Plasmid

    #104635

    Purpose

    Level 2 construct

    Depositor

    Marco Trujillo

    Article

    Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

    Insert

    HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB_EL4
    

    Use

    Tags

    HIS-Ubq, HA-E2, GST-E3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDJSVT91

    Plasmid

    #109305

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - SufI signal (TAT) and His6 tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

    Type

    Empty backbone

    Use

    Tags

    SufI 1-30, 6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBD-0016

    Plasmid

    #12345

    Depositor

    Axel Brunger

    Article

    DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

    Insert

    VCP R313K (Vcp Mouse)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    R313K, I206V*(see below)

    Promoter

    Available since

    Aug. 24, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pCri-13a

    Plasmid

    #61321

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    His-tag and MISTIC-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. K560_AviN_coBirA

    Plasmid

    #129766

    Purpose

    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assays

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Use

    Tags

    Avi-tag GLNDIFEAQKIEWH and His6

    Expression

    Bacterial

    Mutation

    Truncation at 559

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDJSVT89

    Plasmid

    #109303

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

    Type

    Empty backbone

    Use

    Tags

    TamA 1-27, 6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKEW215_AcrIIA13

    Plasmid

    #138281

    Purpose

    Expression of AcrIIA13 protein

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    AcrIIA13
    

    Use

    CRISPR

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 6xHis-MBP-huLb5Cas12a

    Plasmid

    #174684

    Purpose

    Expresses 6xHis-MBP tagged Lb5Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huLb5Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p2CT-Cdi

    Plasmid

    #83044

    Purpose

    Expresses Corynebacterium diphtheriae Cas9 (CdiCas9)

    Depositor

    Jennifer Doudna

    Article

    Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

    Insert

    CdiCas9
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only