We narrowed to 70,741 results for: LAS

1. pLNCX2-NGN2-IRES-GFP

   Plasmid

   #90212

   Purpose

   To convert human fetal lung fibroblasts into induced cholinergic neurons (hiCN) in combination with two small molecules, forskolin and dorsomorphin.

   Depositor

   Chun-Li Zhang

   Article

   Liu et al Nat Commun. 2013;4:2183. doi: 10.1038/nc

   Insert

   NGN2-IRES-GFP (NEUROG2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLdCN

   Plasmid

   #84290

   Purpose

   Express gRNA and Cas9 in Leishmania with Neomycin resistance

   Depositor

   Greg Matlashewski

   Article

   Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

   Insert

   gRNA and Cas9
   

   Use

   CRISPR; Leishmania

   Tags

   Expression

   Mutation

   Promoter

   L. donovani ribosome RNA promoter

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-Flag-NPM1c

   Plasmid

   #131813

   Purpose

   Expresses Flag-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfection

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   NPM1 (NPM1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   This is the cytoplasmic mutant causing AML, where…

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC-GFP-MCC

   Plasmid

   #133307

   Purpose

   "Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisation

   Depositor

   Chris Barnes

   Article

   Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

   Insert

   microcin-V bacteriocin cassette (cvaC E. coli)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   N112D (please see depositors comments)

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSW003-PpsbA-mNeonGreen

   Plasmid

   #205019

   Purpose

   Broad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mNeonGreen is codon optimized for expression in P…

   Promoter

   PpsbA

   Available since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mCherry-FKBP-INPP4BC842A

   Plasmid

   #116865

   Purpose

   Inactive recruitable PI(3,4)P2 4-phosphatase

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   INPP4B (INPP4B Human)
   

   Use

   Tags

   FKBP12 and mCherry

   Expression

   Mammalian

   Mutation

   catalytic cysteine 842 changed to alanine

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOP22-5'UTR-BBa_B0034

   Plasmid

   #216626

   Purpose

   NanoLuc reporter plasmid to optimize chloroplast cell-free systems with RBS BBa_B0034 in 5'UTR

   Depositor

   Henrike Niederholtmeyer

   Article

   Bohm et al ACS Synth Biol. 2024 Aug 16;13(8):2412-2

   Insert

   T7p-5'B0034-NanoLuc-3'TMV
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOP01-UTC-7.0

   Plasmid

   #216625

   Purpose

   NanoLuc reporter plasmid to optimize chloroplast cell-free systems, universal test construct

   Depositor

   Henrike Niederholtmeyer

   Article

   Bohm et al ACS Synth Biol. 2024 Aug 16;13(8):2412-2

   Insert

   T7p-5'g10-NanoLuc-3'TMV
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AMA1-bio

   Plasmid

   #47741

   Purpose

   Expresses enzymatically monobiotinylated full-length AMA1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised AMA1
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-SARS-CoV-2 Nsp3TMC111A

    Plasmid

    #204207

    Purpose

    Mammalian expression of SARS-CoV-2 Nsp3

    Depositor

    Allan Weissman

    Article

    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

    Insert

    SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
    

    Use

    Tags

    FLAGx1

    Expression

    Mammalian

    Mutation

    Cys to Ala in active site of PLpro

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MSP10-bio

    Plasmid

    #47719

    Purpose

    Expresses enzymatically monobiotinylated full-length MSP10 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MSP10
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HDM_omicron_B11529_IDTDNA

    Plasmid

    #204142

    Purpose

    Expression plasmid for SARS-CoV-2 BA.1 variant spike

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    SARS-CoV-2 BA.1 variant spike (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    21 amino acid cytoplasmic tail deletion

    Promoter

    CMV

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NES-PAmCherry-cPHx3

    Plasmid

    #116861

    Purpose

    PI(3,4)P2 biosensor

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    PLEKHA1 (PLEKHA1 Human)
    

    Use

    Tags

    PAmCherry

    Expression

    Mammalian

    Mutation

    amino acids 169-329 (tandem trimer)

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MTRAP-bio

    Plasmid

    #47746

    Purpose

    Expresses enzymatically monobiotinylated full-length MTRAP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MTRAP
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EBA140-bio

    Plasmid

    #47742

    Purpose

    Expresses enzymatically monobiotinylated full-length EBA140 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised EBA140
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV asyn S87A

    Plasmid

    #36056

    Depositor

    Hilal Lashuel

    Article

    Oueslati et al J Neurosci. 2012 Feb 1;32(5):1536-44.

    Insert

    alpha-synuclein S87A (SNCA Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S87A

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

17. Pf113-bio

    Plasmid

    #47729

    Purpose

    Expresses enzymatically monobiotinylated full-length Pf113 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised Pf113
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NES-EGFP-cPHx1

    Plasmid

    #116853

    Purpose

    PI(3,4)P2 biosensor

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    PLEKHA1 (PLEKHA1 )
    

    Use

    Tags

    EGFP and X.leavis map2k1.L(32-44)

    Expression

    Mammalian

    Mutation

    Amino acids 169.329

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSW002-PpsbA-mNeonGreen

    Plasmid

    #205017

    Purpose

    Broad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mNeonGreen is codon optimized for expression in P…

    Promoter

    PpsbA

    Available since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GAMA-bio

    Plasmid

    #47747

    Purpose

    Expresses enzymatically monobiotinylated full-length GAMA ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised GAMA
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA1-Bystin

    Plasmid

    #11869

    Depositor

    Michiko Fukuda

    Article

    Suzuki et al Proc Natl Acad Sci U S A. 1998 Apr 28. 9

    Insert

    Bystin (BYSL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

22. P41-bio

    Plasmid

    #47739

    Purpose

    Expresses enzymatically monobiotinylated full-length P41 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised P41
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSN22

    Plasmid

    #100107

    Purpose

    expresses a chimeric SOX2-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCitrine-P2A-SOX2 fused to a 'strong' VP16 domain (SOX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLG1-puro-sgULK1-1

    Plasmid

    #109004

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ULK1-sgRNA #1 (ULK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV asyn S87E

    Plasmid

    #36057

    Depositor

    Hilal Lashuel

    Article

    Oueslati et al J Neurosci. 2012 Feb 1;32(5):1536-44.

    Insert

    alpha-synuclein S87E (SNCA Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S87E

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

26. pSN69

    Plasmid

    #100125

    Purpose

    expresses putative cMYC DBD with a HA tag

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCerulean-P2A-putative cMYC DBD with a HA tag (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Insert corresponds to the DBD of cMYC

    Promoter

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSN23

    Plasmid

    #100108

    Purpose

    expresses a chimeric SOX2-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCitrine-P2A-SOX2 fused to a 'medium' VP16 domain (SOX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. RAMA-bio

    Plasmid

    #47786

    Purpose

    Expresses enzymatically monobiotinylated full-length RAMA ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised RAMA
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PTEX150-bio

    Plasmid

    #47788

    Purpose

    Expresses enzymatically monobiotinylated full-length PTEX150 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised PTEX150
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MSP9-bio

    Plasmid

    #47740

    Purpose

    Expresses enzymatically monobiotinylated full-length MSP9 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MSP9
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge