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Plasmid#92407PurposehQPRTase harboring R102Q mutationDepositorInserthQPRTase (QPRT Human)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationR102QPromoterT7Available sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprtK171S
Plasmid#92404PurposehQPRTase harbouring K171S mutationDepositorInserthQPRTase (QPRT Human)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationK171SPromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprtR138Q
Plasmid#92411PurposehQPRTase harbouring R138Q mutationDepositorInserthQPRTase (QPRT Human)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationR138QPromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprtR102A
Plasmid#92405PurposehQPRTASE harbouring R102A mutationDepositorInserthQPRTase (QPRT Human)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationR102APromoterT7Available sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;cldn5bCE1-basEgfp
Plasmid#90136Purposecontains cldn5b enhancer sequence and a basal promoter driving expression of eGFPDepositorInsertcldn5bE1basEgfp (cldn5b Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMEsnx5P1gfp
Plasmid#90131PurposeGateway Mid-entry clone containing promoter sequence specific for snx5 driving expression of eGFPDepositorInsertsnx5P1Egfp (snx5 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pACYC_HA-ZmSAE1_ZmSAE2(TRUNC)-FLAG
Plasmid#91945PurposeBacterial expression of maize SAE1 and truncated SAE2DepositorInsertsHA-ZmSAE1
ZmSAE2-FLAG
UseTagsFLAG and HAExpressionBacterialMutationTruncation of ZmSAE2 to mimic a splice variantPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhG-pHG165a
Plasmid#90039PurposeConstitutive expression of a chimeric LacI:GalR transcription repressorDepositorInsertLLhG
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LGhG-pHG165a
Plasmid#90048PurposeConstitutive expression of a chimeric LacI:GalR transcription repressorDepositorInsertLGhG
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMEtmem88aP1gfp
Plasmid#90132PurposeGateway Mid-entry clone containing promoter sequence specific for tmem88a driving expression of eGFPDepositorInserttmem88aP1Egfp (tmem88a Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceJune 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;yrkP1Egfp
Plasmid#90168Purposecontains yrk specific gene promoter driving expression of eGFPDepositorInsertyrkP1Egfp (yrk Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;snx5P1Egfp
Plasmid#90166Purposecontains snx5 specific gene promoter driving expression of eGFPDepositorInsertsnx5P1Egfp (snx5 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;tmem88aP1Egfp
Plasmid#90167Purposecontains tmem88a specific gene promoter driving expression of eGFPDepositorInserttmem88aP1Egfp (tmem88a Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;cldn5bP1Egfp
Plasmid#90158Purposecontains cldn5b specific gene promoter driving expression of eGFPDepositorInsertcldn5bP1Egfp (cldn5b Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;sheCE1-P1Egfp
Plasmid#90150Purposecontains She enhancer sequence, and She specific gene promoter driving expression of eGFPDepositorInsertsheE1P1Egfp (she Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;nrp1bCE1-P1Egfp
Plasmid#90149Purposecontains nrp1b enhancer sequence, and nrp1b specific gene promoter driving expression of eGFPDepositorInsertnrp1bE1P1Egfp
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;sheCE1-basEgfp
Plasmid#90139Purposecontains She enhancer sequence and a basal promoter driving expression of eGFPDepositorInsertsheE1basEgfp (she Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;tmem88aCE1-basEgfp
Plasmid#90135Purposecontains tmem88a enhancer sequence and a basal promoter driving expression of eGFPDepositorInserttmem88aE1basEgfp (tmem88a Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;nrp1bCE1-basEgfp
Plasmid#90134Purposecontains nrp1b enhancer sequence and a basal promoter driving expression of eGFPDepositorInsertnrp1bE1basEgfp
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMEyrkP1gfp
Plasmid#90133PurposeGateway Mid-entry clone containing promoter sequence specific for yrk driving expression of eGFPDepositorInsertyrkP1Egfp (yrk Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMEcldn5bP1gfp
Plasmid#90124PurposeGateway Mid-entry clone containing promoter sequence specific for cldn5b driving expression of eGFPDepositorInsertcldn5bP1Egfp (cldn5b Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
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p5Etmem88aE1
Plasmid#90112Purpose5' Gateway entry clone containing endothelial specific enhancer sequence of tmem88aDepositorInserttmem88aE1 (tmem88a Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJB172
Plasmid#86992PurposeCRISPR/Cas9 in fission yeast using fluoride selection and targetting pil1DepositorInsertgRNA targeting pil1
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3E-FRB(KTF)-HA no-pA
Plasmid#82596Purpose3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (W2101F) without pA for rapamycin/rapalog induced dimerizationDepositorInsertFRB-HA (no pA)
UseTagsExpressionMutationW2101FPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-Laforin Myc 3CS (C250S,C266S,C278S)
Plasmid#84661Purposemammalian expression and/or retrovirus preparationDepositorArticleInsertLaforin (EPM2A Human)
UseRetroviralTagsMycExpressionMammalianMutationPromoter5'LTRAvailable sinceNov. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-Laforin Myc 3CS_new (C266S, C278S, C329S)
Plasmid#84662Purposemammalian expression and/or retrovirus preparationDepositorArticleInsertLaforin (EPM2A Human)
UseRetroviralTagsMycExpressionMammalianMutationPromoter5'LTRAvailable sinceNov. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE30-roGFP1-iR
Plasmid#83314PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertroGFP1-iR
UseTags6xHISExpressionBacterialMutationro1(C48S/S147C/Q204C) plus X insertion, iR betwe…PromoterT5Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-mCE
Plasmid#82459PurposeExpression of myc epitope and murine mRNA Capping Enzyme in mammalian cellsDepositorInsertmRNA capping enzyme (Rngtt Mouse)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
POLN delP_pDEST17
Plasmid#64886Purposebacterial expression of human POLN with a short truncation of the C-terminal proline-rich tailDepositorInsertPOLN (POLN Human)
UseTagsFlag and HisExpressionBacterialMutationwith a short truncation of the C-terminal proline…PromoterT7Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
hFLN29-C/pCMV-14-FLAG (Q391-END)
Plasmid#73590PurposeExpress C-terminal part (Q391- end) of human TRAFD1(FLN29) in mammalian cellsDepositorInsertTRAFD1 (TRAFD1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
hFLN29-Mid/pCMV-14-FLAG (L175-D390)
Plasmid#73589PurposeExpress middle part (L175-D390) of human TRAFD1 in mammalian cellsDepositorInsertTRAFD1 (TRAFD1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
hFLN29-N/pCMV-14-FLAG (1-I210)
Plasmid#73588PurposeExpress N-terminal part (1-I210) of hTRAFD1 (FLN29) in mammalian cellsDepositorInsertTRAFD1 (TRAFD1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry
Plasmid#68276PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry Non functional sensor with phospho-mimicking NLSDepositorInsertSte7DS NLS(4E) mCherry
UseTagsmCherryExpressionYeastMutationPromoterRPS2Available sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gg 3kb Green opsin GFP
Plasmid#72919Purposegreen opsin promoter from chicken (Gallus gallus) gDNA chr26:4,504,913–4,501,931 in galGal4 driving GFPDepositorInsertchicken green opsin promoter
UseTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gg 3kb Green opsin DsRed
Plasmid#72918Purposegreen opsin promoter from chicken (Gallus gallus) gDNA chr26:4,504,913–4,501,931 in galGal4 driving DsRedDepositorInsertchicken green opsin promoter
UseTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - C16orf80 sgRNA 1
Plasmid#70651PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against C16orf80
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - C16orf80 sgRNA 2
Plasmid#70650PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against C16orf80
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - C3orf17 sgRNA 2
Plasmid#70653PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against C3orf17
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only