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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 6,654 results for: SIM;

Showing: 4481 - 4500 of 6654 results

  1. MTK0_054

    Plasmid
    #123980
    Purpose
    Encodes the PhiC31 landing pad on hCLYBL with hygromycin resistance cassette coexpressed with mRuby2 as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PhiC31 LP on hCLYBL (Hygro::mRuby)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123980-plasmid-map-sequence-id-251849

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  2. MTK234_056

    Plasmid
    #123921
    Purpose
    Encodes the spCas9 gRNA GFP dropout expression cassette (human u6 promoter and constant region 3) as a type 234 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    gRNA-GFPDROPOUT-hu6_20N_cr3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123921-plasmid-map-sequence-id-250076

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  3. MTK0_016

    Plasmid
    #123931
    Purpose
    Encodes the phiC31 attB GFP dropout destination vector constitutive NLS::tagBFP as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    Phic31attB tagBFP destination vector
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123931-plasmid-map-sequence-id-250083

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  7. MTK6_013

    Plasmid
    #123867
    Purpose
    Encodes Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) expressing a Blasticidin resistance cassette without a terminator to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    WPRE-Blast (no terminator)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123867-plasmid-map-sequence-id-249818

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  12. pGEM-myr-Citrine-SV40pA-FKF

    Plasmid
    #119865
    Purpose
    Donor cassette containing myr-tagged Citrine and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d
    Insert
    myr-tagged Citrine and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Use
    Tags
    2x myristoylation
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    119865-plasmid-map-sequence-id-239419

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  13. pVR2-m4

    Plasmid
    #84723
    Purpose
    pVR2 but with G to U and A to C mutations following the riboswitch aptamer
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G to U mutation at the first nucleotide after the…
    Promoter
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    84723-plasmid-map-sequence-id-167501

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  14. pVR2-m5

    Plasmid
    #84724
    Purpose
    pVR2 but with an insertion of 1 U immediately following the riboswitch aptamer
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    insertion of 1 U between positions 39 and 40 of a…
    Promoter
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    84724-plasmid-map-sequence-id-167505

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  15. pVR2-m6

    Plasmid
    #84725
    Purpose
    pVR2 but with an insertion of 2 U's immediately following the riboswitch aptamer
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    insertion of 2 Us between positions 39 and 40 of …
    Promoter
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    84725-plasmid-map-sequence-id-167510

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  16. pVR2-m7

    Plasmid
    #84726
    Purpose
    pVR2 but with a G to U mutation immediately following the riboswitch aptamer
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G to U mutation at position 41 of annotated "…
    Promoter
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    84726-plasmid-map-sequence-id-167513

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  17. pVR2-m8

    Plasmid
    #84727
    Purpose
    pVR2 but with two G to U mutations immediately following the riboswitch aptamer
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G to U mutations at positions 40 and 41 of annota…
    Promoter
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    84727-plasmid-map-sequence-id-167518

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  18. pVR

    Plasmid
    #84718
    Purpose
    E. coli RecA promoter followed by preQ1 riboswitch from the 5' UTR of the queC gene from B. subtilis
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    84718-plasmid-map-sequence-id-167475

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  19. pVR2-m1

    Plasmid
    #84720
    Purpose
    pVR2 but with a C to U mutation in the riboswitch aptamer
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C to U mutation at position 13 of annotated "…
    Promoter
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    84720-plasmid-map-sequence-id-167487

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Showing: 4481 - 4500 of 6654 results