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pCDNA4/TO-SPOP-myc (R354H)

Plasmid

#197006

Purpose

Mammalian inducible expression vector for myc-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

myc

Expression

Mammalian

Mutation

R354H

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-SPOP-myc (R221C)

Plasmid

#197005

Purpose

Mammalian inducible expression vector for myc-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

myc

Expression

Mammalian

Mutation

R221C

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-SPOP-myc (BACK)

Plasmid

#197021

Purpose

Mammalian inducible expression vector for myc-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

myc

Expression

Mammalian

Mutation

Y353E

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpH-CeeIF4E3_1-215opt_AB

Plasmid

#148367

Purpose

Bacterial Expression of CeeIF4E3_1-215

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

CeeIF4E3_1-215

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpH-CeeIF4E3_1-215opt-V58AI74A_AB

Plasmid

#148368

Purpose

Bacterial Expression of CeeIF4E3_1-215-V58AI74A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

CeeIF4E3_1-215-V58AI74A

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpH-CeeIF4E3_30-215opt_AB

Plasmid

#148369

Purpose

Bacterial Expression of CeeIF4E3_30-215opt

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

CeeIF4E3_30-215opt

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp1-Hsp2Cas9-Y-P2A-puro

Plasmid

#192132

Purpose

Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1-Hsp2Cas9-Y

Use

AAV

Expression

Mammalian

Mutation

Hsp1-Hsp2Cas9 (Y446A)

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA

Plasmid

#194725

Purpose

based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFB

Depositor

Rudolf Jaenisch

Article

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

AsCpf1 (MAFB, AAVS1 Acidaminococcus sp.)

Use

CRISPR

Expression

Mammalian

Mutation

D908A

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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LentiU6-hIL1RN gRNA_1-MS2-Puro

Plasmid

#192677

Purpose

Lentiviral expression of sgRNA targeting hIL1RN promoter to activate human IL1RN transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human IL1RN activating gRNA #1 (IL1RN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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KBB438

Plasmid

#185092

Purpose

GAL1::Rfa1[1-337, 344-621]-GFP with NLS removed

Depositor

Ken Belanger

Article

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA1

Expression

Yeast

Mutation

Rfa1 with amino acids 338-343 deleted

Promoter

GAL1

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB372

Plasmid

#185084

Purpose

GAL::Rfa1[330-621]-GFP under control of GAL1 promoter

Depositor

Ken Belanger

Article

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA1

Tags

GFP

Expression

Yeast

Mutation

Rfa1 amino acids 330-621

Promoter

GAL1

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-hPPP3R2

Plasmid

#179163

Purpose

Expression vector of human protein phosphatase 3, regulatory subunit B, beta isoform (PPP3R2), CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

Insert

protein phosphatase 3, regulatory subunit B, beta isoform (PPP3R2 Human)

Expression

Mammalian

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mPpp3r2

Plasmid

#179161

Purpose

Expression vector of mouse protein phosphatase 3, regulatory subunit B, beta isoform (Ppp3r2), CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

Insert

protein phosphatase 3, regulatory subunit B, beta isoform (Ppp3r2 Mouse)

Expression

Mammalian

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin-FLN-ELP-His-ybbr

Plasmid

#168039

Purpose

Expresses anticalin targeting CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

3xELP linker, 6x Histag, ddFLN4 (C18S), and ybbr …

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-SdrG-FLN-ELP-His-ybbr

Plasmid

#168047

Purpose

Expresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G

Tags

3xELP linker, 6x Histag, ddFLN4, and ybbr tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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p35S_EVDwt

Plasmid

#167119

Purpose

Plant binary expression vector containing the coding sequence of the Arabidopsis Copia93 retroelement EVADE (AT5G17125) under CaMV 35S promoter.

Depositor

Arturo Marí-Ordóñez, Olivier Voinnet

Article

Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

Insert

EVADE (EVD) (AT5G17125 Mustard Weed)

Expression

Plant

Promoter

CaMV 35S

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pX330_gRNA_IG-DMR-1

Plasmid

#159931

Purpose

Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

Depositor

Anton Wutz

Article

Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

Insert

gRNA mouse IG-DMR

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6, CBh

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_PDCD6IP_BRO1

Plasmid

#109994

Purpose

Protein expression and purification of PDCD6IP_BRO1

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PDCD6IP_BRO1 (PDCD6IP Human)

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_LRP5_Ldl-recept-b_2

Plasmid

#109807

Purpose

Protein expression and purification of LRP5_Ldl-recept-b_2

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

LRP5_Ldl-recept-b_2

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pX330_gRNA_IG-DMR-2

Plasmid

#159932

Purpose

Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

Depositor

Anton Wutz

Article

Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

Insert

gRNA mouse IG-DMR

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6, CBh

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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