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pLPC-NMYC-mTRF1deltaBLM

Plasmid

#64163

Purpose

Retroviral vector expressing mouse TRF1 lacking BLM-binding motifs with N-terminal Myc tag

Depositor

Titia de Lange

Article

Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

Insert

mTRF1 (Terf1 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Deleted amino acids 313-316 and 339-342 (both BLM…

Promoter

CMV

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CHRFAM7ADelta2bp-YFP

Plasmid

#62637

Purpose

mammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with YFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Expression

Mammalian

Mutation

there is a 2-bp deletion in exon 6 of CHRFAM7A an…

Promoter

CMV

Available Since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP8s-WPRE

Plasmid

#162374

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP8f-WPRE

Plasmid

#162376

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Q315L

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG PSAM4-5HT3 HC IRES EGFP

Plasmid

#119740

Purpose

Chemogenetic activator expression plasmid

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Insert

PSAM4 a75HT3 HC (homo) IRES eGFP (CHRNA7, HTR3A Synthetic, Human)

Expression

Mammalian

Mutation

L131G, Q139L, Y217F, R420Q, R424D, R428A

Promoter

CAG

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP8m-WPRE

Plasmid

#162375

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

A25G F286Y

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pET-15b_LC3B

Plasmid

#73949

Purpose

For recombinant expression of human LC3B/MAP1LC3B in E. coli

Depositor

Dieter Willbold

Article

Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

Insert

LC3B (MAP1LC3B Human)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-N2-6MYC-hMID2

Plasmid

#51035

Purpose

mammalian expression of myc-tagged human MID2

Depositor

Timothy Cox

Article

Short et al BMC Cell Biol. 2002;3:1. Epub 2002 Jan 4

Insert

MID2 (MID2 Human)

Tags

MYC

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-DDX11-Flag-WT

Plasmid

#120727

Purpose

Expression of a Flag-tagged version of DDX11 WT in mammalian cells

Depositor

Francesca Pisani

Article

Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622.

Insert

DDX11 (DDX11 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-sumo-NSP12 (SARS-CoV-2)

Plasmid

#159107

Purpose

The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

Depositor

Thomas Tuschl

Article

Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

Insert

NSP12

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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