We narrowed to 43,301 results for: cha;

1. AAVS-HsProm1s1-StayGold

   Plasmid

   #211333

   Purpose

   Vector for stable mammalian gene editing with C-terminally StayGold tagged human Prom1s1 (WT)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Prominin 1 (PROM1 Human)
   

   Use

   AAV

   Tags

   StayGold

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIBA3-SpyCatcher-sfGFP

   Plasmid

   #107420

   Purpose

   For production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.

   Depositor

   Jack Leo

   Article

   Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

   Insert

   SpyCatcher
   

   Use

   Tags

   His tag, StrepII, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Tet

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-IRAlus-Apobec3G

   Plasmid

   #92349

   Purpose

   Plasmid for expression of GFP gene with a Apobec3G gene intron derived IR Alu element containing 3'UTR.

   Depositor

   Gordon Carmichael, Ling-Ling Chen

   Article

   Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

   Insert

   Apobec3G gene intron derived IR Alu element containing 3'UTR (APOBEC3G Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. NAX_CAGGS_CAS9_m4_VPR_KanR neo

   Plasmid

   #167885

   Purpose

   PiggyBac compatible plasmid expressing dCas9-VPR

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. OPA1-isoform3

   Plasmid

   #70175

   Purpose

   Mammalian expression of hsOPA1 isoform 3

   Depositor

   David Chan

   Article

   Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

   Insert

   hsOPA1 isoform 3 (OPA1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pEGFP-Alus-Apobec3G

   Plasmid

   #92348

   Purpose

   Plasmid for expression of GFP gene with a Apobec3G gene intron derived Alu element containing 3'UTR.

   Depositor

   Gordon Carmichael, Ling-Ling Chen

   Article

   Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

   Insert

   Apobec3G gene intron derived Alu element containing 3'UTR (APOBEC3G Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRR-ERbeta-5Z

   Plasmid

   #23062

   Depositor

   Charles Miller

   Article

   Miller et al Plasmid. 2009 Dec 3. ():.

   Insert

   ERbeta (ESR2 Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pFSW GAP43-jRGECO1a P2A Syp-Halo

   Plasmid

   #185565

   Purpose

   Expression of axonally-targeted jRGECO1a, a red Ca2+ sensor, along with synaptophysin-HaloTag in neuronal cells, separated by a P2A sequence

   Depositor

   Edwin Chapman

   Article

   Bradberry et al J Physiol. 2022 Feb;600(3):645-654. doi:

   Insert

   GAP43-jRGECO1a P2A Syp-Halo
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mutation

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. OPA1-isoform4

   Plasmid

   #70176

   Purpose

   Mammalian expression of hsOPA1 isoform 4

   Depositor

   David Chan

   Article

   Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

   Insert

   hsOPA1 isoform 4 (OPA1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pET21-pelB-LaG-30

    Plasmid

    #172754

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-30
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3 PARL-FLAG-CT S277G

    Plasmid

    #13615

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed catalytic Serine 277 to Alanine

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

12. OPA1-isoform6

    Plasmid

    #70178

    Purpose

    Mammalian expression of hsOPA1 isoform 6

    Depositor

    David Chan

    Article

    Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

    Insert

    hsOPA1 isoform 6 (OPA1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP

    Plasmid

    #45579

    Purpose

    Expresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP tracker

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Insert

    coY2 Ani K227M
    

    Use

    Lentiviral

    Tags

    HA, IRES BFP, and NLS

    Expression

    Mammalian

    Mutation

    co indicates codon optimization for mammalian exp…

    Promoter

    SFFV

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-TRE-DIO-mTurquoise2

    Plasmid

    #99115

    Purpose

    An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mTurquoise2

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    DIO-mTurquoise2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTG-mTDG

    Plasmid

    #52266

    Purpose

    Bacterial expression of mouse TDG with C-terminal GST-tag

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    TDG (Tdg Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p-mCherry-C1-GtACR1

    Plasmid

    #85464

    Purpose

    Natural anion conducting Channelrhodopsin with cyan absorption. Closing kinetics 140ms. High conductivity. Codon optimized for mammalian expression (human/mouse)

    Depositor

    Peter Hegemann

    Article

    Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.

    Insert

    Synthetic construct ACR1 gene
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (+enhancer)

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMpGWB215

    Plasmid

    #68606

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Nuclear localization signal-tagged Citrine (Citri…

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-HA-TRAIL-R1

    Plasmid

    #61382

    Purpose

    Expresses HA tagged TRAIL receptor 1

    Depositor

    Francis Chan

    Article

    Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

    Insert

    TRAIL receptor 1
    

    Use

    Tags

    HA and TNF receptor leader peptide

    Expression

    Mammalian

    Mutation

    TRAIL-R1 leader peptide (aa1-23) replaced with TN…

    Promoter

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pET21-pelB-LaM-8

    Plasmid

    #172772

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-8
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AcpP pet29a C-terminal His Tag

    Plasmid

    #75016

    Purpose

    Expresses the acyl carrier protein (ACP) from the E. coli fatty acid synthase

    Depositor

    Michael Burkart

    Article

    Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

    Insert

    AcpP (acpP E. coli)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

21. p-mCherry-C1-GtACR2

    Plasmid

    #85463

    Purpose

    Natural anion conducting Channelrhodopsin with blue absorption. Closing kinetics 60ms. High conductivity. Codon optimized for mammalian expression (human/mouse)

    Depositor

    Peter Hegemann

    Article

    Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.

    Insert

    Synthetic construct ACR2 gene
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (+enhancer)

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. NFAST(low)-mApple-6xHp

    Plasmid

    #214412

    Purpose

    Lipid droplet localization of low-affinity NFAST fused to mApple-6xHp

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    RspA-N
    

    Use

    Tags

    mApple-6xHp

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPPEM

    Plasmid

    #162467

    Purpose

    Destination Expression vector for monocot plant primer editing

    Depositor

    Adam Bogdanove

    Article

    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

    Insert

    Cas9H840A
    

    Use

    Tags

    MLV reverse transcriptase

    Expression

    Plant

    Mutation

    Change histidine 840 to Alanine

    Promoter

    maize Ubi

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCOLA-Spec-yibDp-Erv1p

    Plasmid

    #212094

    Purpose

    Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    Sulfydryl oxidase (ERV1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pT-GEM(0)

    Plasmid

    #62891

    Purpose

    Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYpet-PBD

    Plasmid

    #22781

    Depositor

    Klaus Hahn

    Article

    Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

    Insert

    PAK binding domain (PAK1 Human)
    

    Use

    Tags

    Ypet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUSE-Src-YF-UniRapR-mCerulean-myc

    Plasmid

    #45381

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

    Insert

    Src-YF-UniRapR-mCerulean-myc
    

    Use

    Tags

    Myc, Myr, and mCerulean

    Expression

    Mammalian

    Mutation

    changed mSrc Tyrosine 529 to Phenylalanine

    Promoter

    CMV

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRK5 CASTOR1-HA Cterm

    Plasmid

    #84487

    Purpose

    overexpression of CASTOR1 with HA tag

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR1 (GATSL3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

29. OVA-miSFIT-17-T

    Plasmid

    #124677

    Purpose

    Tuning expression of cytosolic Ovalbumin coupled to EGFP

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    Cyto-Ovalbumin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Truncated OVA for cytoplasmic expression

    Promoter

    minimal CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDS275

    Plasmid

    #34972

    Purpose

    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(T406A, T407A, I532A mutations).

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T406A, T407A, I532A

    Promoter

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. EC71100

    Plasmid

    #154068

    Purpose

    Level 0 Golden Gate vector, pHvHSP17

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    pHvHSP17
    

    Use

    Standard cloning vector used by geneart for synth…

    Tags

    Expression

    Mutation

    All BsaI, Esp3I and BpiI restriction sites were r…

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBSc + MShal

    Plasmid

    #16166

    Depositor

    Larry Salkoff

    Article

    Pak et al Proc Natl Acad Sci U S A. 1991 May 15. 8

    Insert

    Mus musculus potassium voltage-gated channel (Kcnd1 Mouse)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

33. Pak3

    Plasmid

    #99510

    Purpose

    to express Pak3 in mammalian system

    Depositor

    Dong-Yan Jin

    Article

    Chan et al Retrovirology. 2013 Apr 26;10:47. doi: 1

    Insert

    Pak3 (Pak3 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Dlx5

    Plasmid

    #15538

    Depositor

    John Rubenstein

    Article

    Zerucha et al J Neurosci. 2000 Jan 15. 20(2):709-21.

    Insert

    Dlx5 (Dlx5 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 20, 2007

    Availability

    Academic Institutions and Nonprofits only

35. pCDNA/golgin-160/myc

    Plasmid

    #180632

    Purpose

    Expression of WT golgin-160

    Depositor

    Carolyn Machamer

    Article

    Hicks et al J Biol Chem. 2002 Sep 27. 277(39):35833-

    Insert

    golgin-160 (GOLGA3 Human)
    

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCyPet-Rac1(WT)

    Plasmid

    #22782

    Depositor

    Klaus Hahn

    Article

    Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

    Insert

    Rac1 (wt) (RAC1 Human)
    

    Use

    Tags

    CyPet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

37. pET21-pelB-LaM-2

    Plasmid

    #172768

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET21-pelB-LaG-41-4GS-LaG-2

    Plasmid

    #172766

    Purpose

    Bacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-41-4GS-LaG-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET15b/bPanK

    Plasmid

    #73619

    Purpose

    Produces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)

    Depositor

    T. J. Kappock, Suzanne Jackowski

    Article

    Calder et al J Biol Chem. 1999 Jan 22;274(4):2014-20.

    Insert

    pantothenate kinase (coaA Escherichia coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    relative to MG1655 sequence, changes glutamate-5 …

    Promoter

    T7

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. NAX_CAGGS_CAS9_m4_VPR_mcherry

    Plasmid

    #167886

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only