-
Plasmid#159064PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0415
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0301:Dendra
Plasmid#159050PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0301
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0225:Dendra
Plasmid#159043PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0225
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0710:Dendra
Plasmid#159020PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0710
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0384:Dendra
Plasmid#159013PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0384
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0229:Dendra
Plasmid#159005PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0229
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0024:Dendra
Plasmid#158997PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0024
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRM209 LTP (Luciferase tdTomato Puro)
Plasmid#174723PurposeExpresses the firefly luciferase gene, E2A, tdTomato, T2A, and the puromycin resistance gene.DepositorInsertsFirefly Luciferase
tdTomato
pac
UseLentiviral and LuciferaseTagsE2A linker and T2A linkerExpressionMammalianMutationPromoterMND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC-G418-YR
Plasmid#61767PurposeYeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing nptII (neomycin phosphotransferase II) selectable marker on transfer DNA (TDNA).DepositorInsertsnptII gene
2 micron origin or replication and URA3 gene for S. cerevisiae
UseTernary vector for agrobacterium mediated transfo…TagsExpressionMutationPromotertrpC promoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBT272_(pRosa26-GTET)
Plasmid#36882DepositorInsertsGFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator
diphteria toxin A
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNK2657
Plasmid#219741PurposeMoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiensDepositorInserthispidin- synthase from Mycena citricolor
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMEL13
Plasmid#107919Purposekan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pROS12
Plasmid#107926Purposehph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pROS13
Plasmid#107927Purposekan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIFEX-A2aGH
Plasmid#160542PurposepITY4 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.DepositorInsertUseTagsExpressionYeastMutationN/APromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNK3071
Plasmid#219755PurposeMoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.DepositorInsertmcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRM210 LGP (Luciferase GFP Puro)
Plasmid#174722PurposeExpresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance geneDepositorInsertsFirefly Luciferase
eGFP
pac
UseLentiviral and LuciferaseTagsE2A linker and F2A linkerExpressionMammalianMutationPromoterMND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
*MtbCM-HiBiT-His
Plasmid#228028PurposeExpresses secreted Mycobacterium tuberculosis (M. tb) chorismate mutase (CM) containing a C-terminal HiBiT sequence and His tagDepositorInsertRv1885c Chorismate Mutase (Rv1885c Mycobacterium tuberculosis H37Rv)
UseTags10xHis-tag with thrombin site and HiBiT TagExpressionBacterialMutationPromoterT7 PromotorAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-dCas9-p300-P2A-PuroR
Plasmid#83889PurposeLentiviral Sp dCas9-p300-P2A-PuroRDepositorInsertsS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
pac from Streptomyces alboniger
UseCRISPR and LentiviralTagsFlag and nucleoplasmin NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEFSAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHL-EF1a-SphcCas9(D10A)-iP-A
Plasmid#60600PurposeExpresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.DepositorInsertsCRISPR Cas9 D10A
puromycin resistance gene
UseCRISPRTagsExpressionMammalianMutationChanged Asp 10 to Ala, Codon usage optimized for …PromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only