We narrowed to 6,669 results for: PROC

1. SF-SON IRES Puro

   Plasmid

   #214402

   Purpose

   Expresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)

   Depositor

   Tanja Kuhlmann

   Article

   Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. SF-SON IRES Tomato

   Plasmid

   #214403

   Purpose

   Expresses human SOX10, OLIG2, NKX6.2 and tdTomato

   Depositor

   Tanja Kuhlmann

   Article

   Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

   Plasmid

   #207878

   Purpose

   AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

   Depositor

   Yeh-Hsing Lao

   Article

   Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21b(+)-Is-PETase-W159H-S238F

   Plasmid

   #112203

   Purpose

   pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12

   Depositor

   Christopher Johnson, Gregg Beckham

   Article

   Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

   Insert

   PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
   

   Tags

   6XHIS

   Expression

   Bacterial

   Mutation

   W159H, S238F

   Promoter

   N/A

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-21b(+)-Is-PETase-W185A

   Plasmid

   #112204

   Purpose

   pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12

   Depositor

   Christopher Johnson, Gregg Beckham

   Article

   Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

   Insert

   PETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
   

   Tags

   6XHIS

   Expression

   Bacterial

   Mutation

   W185A

   Promoter

   N/A

   Available Since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV:ITR-EF1aCore-SaCas9-single(U6-sgRNA(backbone))-ITR

   Plasmid

   #207880

   Purpose

   AAV vector encoding EF1a core promoter-driven SaCas9 and single sgRNA cloning site w/ the engineered Sa Guide scaffold variant (BbsI for sgRNA cloning).

   Depositor

   Yeh-Hsing Lao

   Article

   Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBABEpuro-gateway

   Plasmid

   #51070

   Purpose

   gateway adapted mammalian expression vector

   Depositor

   Matthew Meyerson

   Article

   Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. SARS-CoV2 Nucleocapsid

   Plasmid

   #153201

   Purpose

   Expresses myc-tagged nucleocapsid (N) from SARS-CoV2 in mammalian cells

   Depositor

   Peter Klein

   Article

   Liu et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

   Insert

   Nucleocapsid from SARS-CoV-2 (N severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
   

   Tags

   Myc tag (5X)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 15, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pCJ189

   Plasmid

   #162666

   Purpose

   pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   codon optimized for expression in E. coli K12

   Promoter

   T7/lac

   Available Since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmEos2-N1 GBP (GBP-mEos2)

    Plasmid

    #162876

    Purpose

    Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy

    Depositor

    Frederic Meunier

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    GFP Binding Protein tagged with mEos2
    

    Tags

    mEos2

    Expression

    Mammalian

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-pBG-mCRE98-NLS-EGFP-WPRE

    Plasmid

    #239977

    Purpose

    adeno-associated virus (AAV) encoding enhanced green fluorescent protein (eGFP) under motor neuron-selective control of minimal beta globin promoter (pBG) and CRE98

    Depositor

    Sinisa Hrvatin

    Article

    Nagy et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    EGFP (eGFP Synthetic)
    

    Use

    AAV

    Promoter

    pBG + mCRE98

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5 frt/to N-BioTAP-C-BRD4-NUT

    Plasmid

    #171630

    Purpose

    Expresses BioTAP-tagged BRD4-NUT in mammalian cells. Can be inserted into a FRT site by co-transfection with a plasmid encoding Flp recombinaase.

    Depositor

    Christopher A French, Chris French

    Article

    Alekseyenko et al Proc Natl Acad Sci U S A. 2017 May 23;11

    Insert

    BRD4-NUT (BRD4, NUTM1 Human)
    

    Tags

    BioTAP

    Expression

    Mammalian

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-TO/CMV-puro-DD-PAmCherry1-KRas G12D

    Plasmid

    #177320

    Purpose

    Lentiviral vector expressing PAmCherry1-tagged KRas 4B G12D mutant, with an N-terminal dimerization domain (DD).

    Depositor

    Steven Chu, Xiaolin Nan

    Article

    Nan et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

    Insert

    KRAS 4B (KRAS Human)
    

    Use

    Lentiviral

    Tags

    FKBP-derived dimerization domain (DD, or DmrB) an…

    Expression

    Mammalian

    Mutation

    G12D

    Promoter

    TetOn-CMV

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCJ202

    Plasmid

    #162675

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    W159C, S238C; codon optimized for expression in E…

    Promoter

    T7/lac

    Available Since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRE3G-GSX2-IRES2-EBF1

    Plasmid

    #96966

    Purpose

    Dox-inducible expression of Gsx2 and Ebf1 in mammalian cells

    Depositor

    Elena Cattaneo

    Article

    Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Insert

    GSX2 and EBF1 (bicistronic insert) (Gsx2, Ebf1 Mouse)
    

    Tags

    FLAG (on EBF1)

    Expression

    Mammalian

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCJ191

    Plasmid

    #162668

    Purpose

    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEXP5-aHL_spytag_his

    Plasmid

    #107334

    Purpose

    Monomeric SpyTag-6×-His alpha-hemolysin

    Depositor

    George Church

    Article

    Stranges et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    alpha-hemolysin-SpyTag-6xHis
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. S10A mutant of H3S10ph biosensor

    Plasmid

    #120810

    Purpose

    FRET biosensor. Eliminating the H3 Serine 10 phosphorylation site in H3S10ph biosensor to abolish the detection capability

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa) S10A-YFP
    

    Expression

    Mammalian

    Mutation

    Serine 10 is mutated to Alanine, and Threonine 3,…

    Promoter

    CMV

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCJ206

    Plasmid

    #162679

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating the E226T mutation to the putative lipase box.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), incorporating the E226T mutation to the putative lipase box
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    E226T; codon optimized for expression in E. coli …

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCJ211

    Plasmid

    #162684

    Purpose

    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with deletion of the predicted signal peptide (Lys2:Gly19), with C-terminal His tag, codon optimized for expression in E. coli K12.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) without 19 residue signal peptide
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    deltaK2:G19; codon optimized for expression in E.…

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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