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We narrowed to 6,501 results for: nls

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Showing: 4521 - 4540 of 6501 results

  1. pEMS1052

    Plasmid
    #29004
    Purpose
    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple112 (HCRT Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Cre-EGFP-NLS
    Available since
    Aug. 4, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    29004-plasmid-map-sequence-id-391177

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  2. pSpdCas9-huTET1CD-T2A-mCherry(PX458)

    Plasmid
    #129027
    Purpose
    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Publication
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-huTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    129027-plasmid-map-sequence-id-256129

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  3. 3xFLAG-dCas9/MSCV-EGFP

    Plasmid
    #82613
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al Genes Cells. 2018 Feb 26. doi: 10.1111/g
    Insert
    3xFLAG-dCas9
    Use
    Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    Sept. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    82613-plasmid-map-sequence-id-298740

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  4. PX380 Treponema denticola Cas9

    Plasmid
    #68317
    Purpose
    Treponema denticola Cas9
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TdCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    68317-plasmid-map-sequence-id-119529

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  7. pCMV NucleoBAS

    Plasmid
    #62861
    Purpose
    Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET
    Depositor
    Stan van de Graaf
    Publication
    van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NucleoBAS
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    62861-plasmid-map-sequence-id-103771

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  8. pEJS-HZ79 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26_V2

    Plasmid
    #199261
    Purpose
    Optimized single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene. This optimized construct showed improved in vivo editing efficiency.
    Depositor
    Erik Sontheimer
    Publication
    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.
    Insert
    NmeABE8e
    Use
    AAV and CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Promoter
    U1a promoter
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    199261-plasmid-map-sequence-id-389819

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  10. pY130 (pcDNA3.1-U6-huBsCpf1)

    Plasmid
    #92306
    Purpose
    Expression of humanized BsCpf1 and crRNA
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huBsCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92306-plasmid-map-sequence-id-181649

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  11. pY127 (pcDNA3.1-U6-huTsCpf1)

    Plasmid
    #92303
    Purpose
    Expression of humanized TsCpf1 and crRNA
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huTsCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92303-plasmid-map-sequence-id-181636

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  12. pY111 (pcDNA3.1-huTsCpf1)

    Plasmid
    #92267
    Purpose
    Expression of humanized TsCpf1
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huTsCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    July 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    92267-plasmid-map-sequence-id-183046

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  15. pY128 (pcDNA3.1-U6-huMb2Cpf1)

    Plasmid
    #92304
    Purpose
    Expression of humanized Mb2Cpf1 and crRNA
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huMb2Cpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92304-plasmid-map-sequence-id-181652

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  16. pYPQAT57

    Plasmid
    #223429
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223429-plasmid-map-sequence-id-453502

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  20. pTE4254

    Plasmid
    #89050
    Purpose
    Expresses St chimeric gRNA and human codon-optimized StCas9
    Depositor
    Ervin Welker
    Publication
    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    St chimeric gRNA
    hStCas9
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV and human U6
    Available since
    April 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    89050-plasmid-map-sequence-id-176333

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Showing: 4521 - 4540 of 6501 results