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(Plasmid #82613)


Item Catalog # Description Quantity Price (USD)
Plasmid 82613 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6421
  • Total vector size (bp) 10822
  • Vector type
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • Mutation
    human codon-optimized, D10A + H840A
  • Promoter LTR
  • Tags / Fusion Proteins
    • 3xFLAG tag (N terminal on insert)
    • NLS (nuclear localization signal) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hpa I (destroyed during cloning)
  • 3′ cloning site Hap I (destroyed during cloning)
  • 5′ sequencing primer MSCV (5'-CCCTTGAACCTCCTCGTTCGACC-3')
  • 3′ sequencing primer EGFP-N (5'-CGTCGCCGTCCAGCTCGACCAG-3)
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3xFLAG-dCas9/MSCV-EGFP was a gift from Hodaka Fujii (Addgene plasmid # 82613 ; ; RRID:Addgene_82613)
  • For your References section:

    Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis. Fujita T, Kitaura F, Oji A, Tanigawa N, Yuno M, Ikawa M, Taniuchi I, Fujii H. Genes Cells. 2018 Feb 26. doi: 10.1111/gtc.12573. 10.1111/gtc.12573 PubMed 29480524