We narrowed to 14,439 results for: NTS

1. pFG2

   Plasmid

   #87250

   Purpose

   gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT). Also known as pANCH1-Nat

   Depositor

   Kerstin Bystricky

   Article

   Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

   Insert

   Nat
   

   Tags

   parS segments

   Expression

   Yeast

   Promoter

   TEF

   Available Since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTPH405c

   Plasmid

   #185726

   Purpose

   Directed evolution of Cas9 adenine base editors with altered PAM compatibility

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   gIII(N)-NpuN-NpuC-gIII(C) single PAM linker
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPEG

   Plasmid

   #162466

   Purpose

   Entry vector for gRNA insertion in plant prime editing expression vector

   Depositor

   Adam Bogdanove

   Article

   Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

   Type

   Empty backbone

   Use

   Entry vector for grna

   Promoter

   CmYLCV

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. EMTB-mNeonGreen

   Plasmid

   #137802

   Purpose

   Visualization of microtubules

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   Ensconsin microtubule binding domain
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGR

   Plasmid

   #46002

   Purpose

   Reporter plasmid for measuring terminator strength by inserting terminator of interest between the GFP and RFP, expression driven by a single PBAD promoter inducible by arabinose

   Depositor

   Christopher Voigt

   Article

   Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PBAD

   Available Since

   June 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. S100A6

   Plasmid

   #196217

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses S100A6.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   S100A6 (S100A6 Human)
   

   Expression

   Bacterial

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK1043

   Plasmid

   #8835

   Depositor

   David Waugh

   Article

   Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

   Insert

   tobacco etch virus protease, catalytic domain
   

   Tags

   MBP and polyarginine

   Expression

   Bacterial

   Mutation

   Autolysis-resistant S219V mutant

   Available Since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSYNCMV tAKARα

   Plasmid

   #119921

   Purpose

   AAV construct with tAKARα being expressed under the human synapsin promotor with WPRE enhancer.

   Depositor

   Haining Zhong

   Article

   Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

   Insert

   tAKARα
   

   Use

   AAV

   Available Since

   April 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3xFLAG-TYR

   Plasmid

   #32780

   Depositor

   Ruth Halaban

   Article

   Halaban et al J Invest Dermatol. 2002 Aug;119(2):481-8

   Insert

   Tyrosinase (TYR Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

10. Lenti-EF1alpha-VP64-dCas9-p300_Blast

    Plasmid

    #192654

    Purpose

    3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    VP64-dCas9-p300
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A; H840A in Cas9

    Promoter

    EF1a

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. mTurquoise2-LaminB

    Plasmid

    #98830

    Purpose

    In vivo visualization of the nuclear envelope (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    LaminB
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    CMV

    Available Since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRU295

    Plasmid

    #167694

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::Cas9i
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA

    Plasmid

    #63166

    Purpose

    GAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-HA"
    

    Tags

    N-myristoylation

    Expression

    Insect

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-SpCas9-P2A-puro

    Plasmid

    #214358

    Purpose

    Expresses SpCas9 and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SpCas9 and puromycin resistance genes
    

    Expression

    Mammalian

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. hCdh1 (1-125)

    Plasmid

    #8508

    Depositor

    Joan Ruderman

    Article

    Crane et al J Cell Sci 2004 Dec 1;117(Pt 25):5975-83

    Insert

    hCdh1 (1-125) (FZR1 Human)
    

    Use

    Retroviral

    Tags

    His

    Expression

    Mammalian

    Mutation

    Cdh1 N-terminus. Dominant Negative mutant.

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pCMU-PMr

    Plasmid

    #61180

    Purpose

    Fluorescent reporter for plasma membrane AtPIP2a-mCherry expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    AtPIP2a
    

    Tags

    mCherry

    Expression

    Plant

    Promoter

    AtUBQ10

    Available Since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTBL3478 PB-PEmax-mTagBFP2

    Plasmid

    #226664

    Purpose

    PiggyBac cargo vector encoding PEmax and mTagBFP2

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Inserts

    PEmax
    mTagBFP2
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG and UBC

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pComb3XLambda

    Plasmid

    #63892

    Purpose

    Backbone pComb3X containing PCR template for human antibody lambda light chain constant region

    Depositor

    Carlos Barbas

    Article

    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

    Insert

    human antibody lambda light chain (IGLL5 Human)
    

    Use

    Phage display

    Expression

    Bacterial

    Promoter

    lacZ

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

    Plasmid

    #175526

    Purpose

    Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lifeact-HaloTag11 and Tomm20-HaloTag7
    

    Expression

    Mammalian

    Mutation

    HaloTag11 = HaloTag7-M175W

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-EF1alpha-VP64-dCas9-VP64_Blast

    Plasmid

    #192650

    Purpose

    3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    VP64-dCas9-VP64
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1a

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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