We narrowed to 19,379 results for: INO

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pATK (pKD-G9)

Plasmid

#62688

Purpose

Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

tdTomato/amiR-eGFP123/amiR-eGFP419

Use

RNAi

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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S. aureus DHPS

Plasmid

#64079

Purpose

Expression of Staphylococcus aureus dihydropteroate synthase

Depositor

Vern Schramm

Article

Stratton et al ACS Chem Biol. 2015 Oct 16;10(10):2182-6

Insert

dihydropteroate synthase

Tags

Histidine tag

Expression

Bacterial

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
dEos-Vinculin-14

Plasmid

#57568

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Betzig et al Science. 2006 Sep 15;313(5793):1642-5. E

Insert

Vinculin (VCL Human)

Tags

dEos

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pSG5-(nic)p85

Plasmid

#55720

Purpose

eukaryotic expression of both SH2 and the inter-SH2 domain of murine p85α.

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p85α

Expression

Mammalian

Mutation

SH2 and the inter-SH2 domain

Promoter

T7

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM1-C103A

Plasmid

#58419

Purpose

Expresses murine IFITM1-C103A with an N-terminal HA tag in mammalian cells.

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteine 103 to Alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM1-C83A

Plasmid

#58418

Purpose

Expresses murine IFITM1-C83A with an N-terminal HA tag in mammalian cells.

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteine 83 to Alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM1-C50A

Plasmid

#58417

Purpose

Expresses murine IFITM1-C50A with an N-terminal HA tag in mammalian cells.

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteine 50 to Alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM1-C49A

Plasmid

#58416

Purpose

Expresses murine IFITM1-C49A with an N-terminal HA tag in mammalian cells

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteine 49 to Alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
p416GAL1-Skn7t-L-IFP-F[1] (Yeast)

Plasmid

#52896

Purpose

Skn7t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Skn7t-L-IFP-F[1]

Tags

L-IFP-F[1]

Expression

Yeast

Promoter

GAL1

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pGEM-and4 in situ probe

Plasmid

#52105

Purpose

actinodin 4 and4 antisense in situ probe linearize w/ AatII SP6 pol

Depositor

Didier Stainier

Article

Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

Insert

actinodin 4 in situ probe (and4 Zebrafish)

Expression

Bacterial

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGEM-and2 in situ probe

Plasmid

#52103

Purpose

actinodin 2 and2 antisense in situ probe linearize w/ ApaI SP6 pol

Depositor

Didier Stainier

Article

Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

Insert

actinodin 2 in situ probe (and2 Zebrafish)

Expression

Bacterial

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGEM-and3 in situ probe

Plasmid

#52104

Purpose

actinodin 3 and3 antisense in situ probe linearize w/ ApaI SP6 pol

Depositor

Didier Stainier

Article

Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

Insert

actinodin 3 in situ probe (and3 Zebrafish)

Expression

Bacterial

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pMB38-HF380deltaMTBD

Plasmid

#37333

Depositor

Takahide Kon

Article

Kon et al Nature. 2012 Mar 7;484(7394):345-50. doi

Insert

Cytoplasmic Dynein Heavy Chain (dhcA Dictyostelium discoideum)

Use

Dictyostelium expression

Tags

His6 and FLAG tag

Mutation

deleted amino acids 1-1387 and replaced amino aci…

Promoter

TRE-Pmin

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only
pLXSN-p85α-ΔiSH2-N

Plasmid

#39502

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

p85 ΔiSH2-N (PIK3R1 Bovine)

Use

Retroviral

Expression

Mammalian

Mutation

ΔiSH2-N = deletion of AA478-513

Available Since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only
p40deltaPX (w/out aa1-135)

Plasmid

#19019

Depositor

Michael Yaffe

Article

Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

Insert

p40phox (NCF4 Human)

Tags

His

Expression

Bacterial

Mutation

aa136-148 (PX domain, truncated)

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
pN006

Plasmid

#188470

Purpose

Lentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)

Depositor

Le Cong

Article

Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

Insert

AsCas12a

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-hCYP26A1P-E4-mCherry

Plasmid

#135478

Purpose

Human short form promoter of human gene in pGL3-Basic-mCherry plasmid vector (Namely, E4.2)

Depositor

Catharine Ross

Article

Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

Insert

mCherry Open reading Frame

Use

Unspecified

Promoter

Short from promoter of human CYP26A1 (312 bp)

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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dCAS9-VP64_GFP

Plasmid

#61422

Purpose

Expresses dCAS9-VP64 activator with 2A GFP

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Concentrated Lentiviral Prep

Insert

dCAS9(D10A,H840A)-VP64_2A_GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and H840A in Cas9

Promoter

EF1A

Available Since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pJRH-1179 U6-reci Gag-Cas9 v2

Plasmid

#201914

Purpose

Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-Cas9 v2

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pXR001: EF1a-CasRx-2A-EGFP

Plasmid

#109049

Purpose

CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting

Depositor

Patrick Hsu

Article

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Insert

CasRx

Use

CRISPR and Lentiviral

Tags

2A-eGFP, HA, and NLS

Expression

Mammalian

Promoter

EF1A

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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