Addgene

We narrowed to 13,034 results for: Mpl

Showing: 4541 - 4560 of 13034 results

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pMaCTag-P16

Plasmid

#120027

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Dronpa3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Dronpa3

Use

Pcr template

Tags

Dronpa3

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTGC-U6.3

Plasmid

#112810

Purpose

PCR template vector for amplifying gRNAcore-pU6.2 promoter fragment

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Insert

U6:3-gRNAcore

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pRPL28 CaURA3 (pNTI592)

Plasmid

#115431

Purpose

URA3-marked RPS28

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
URA3

Use

Tags

Expression

Yeast

Mutation

KpnI site in intron

Promoter

Ashbya gossypii and endogenous

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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GST-GNU-N

Plasmid

#112972

Purpose

Expresses fusion of GST and N terminal amino acids of GNU

Depositor

Terry Orr-Weaver

Article

Lee et al Genes Dev. 2003 Dec 1;17(23):2979-91. do

Insert

gnu (gnu Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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gnu-GFP 9A

Plasmid

#113006

Purpose

P element vector for transposon insertion of gnu-GFP 9A into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

full length gnu (gnu Fly)

Use

P insertion vector

Tags

GFP

Expression

Mutation

9 CycB/CDK1 sites mutated to A

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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gnu-GFP WT

Plasmid

#113005

Purpose

P element vector for transposon insertion of gnu-GFP WT into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

full length gnu (gnu Fly)

Use

P insertion vector

Tags

GFP

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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6xmyc_cort_UAS-P

Plasmid

#112991

Purpose

P element vector for transposon insertion of Myc-tagged Drosophila cort (Cdc20) into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Pesin et al PLoS Genet. 2007 Nov;3(11):e202. doi: 10

Insert

cort (Cdc20) (cort Fly)

Use

P insertion vector

Tags

6X myc epitope

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1 gnu 9A

Plasmid

#113000

Purpose

For protein expression and purification of full-length Drosophila gnu 9A

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

full length gnu (gnu Fly)

Use

Tags

Expression

Bacterial

Mutation

9 CycB/CDK1 sites mutated to A

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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full length png in pGEX4t

Plasmid

#112974

Purpose

For protein expression and purification of full-length Drosophila png

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

png (png Fly)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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gnu cDNA LD12084

Plasmid

#112973

Purpose

Cloning vector carrying the gnu cDNA

Depositor

Terry Orr-Weaver

Article

Lee et al Genes Dev. 2003 Dec 1;17(23):2979-91. do

Insert

gnu (gnu Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R7R8

Plasmid

#111041

Purpose

Plasmid expresses the talin2 rod domains R7 and R8

Depositor

Ben Goult

Article

Bouchet et al Elife. 2016 Jul 13;5. doi: 10.7554/eLife

Insert

TLN2 (Tln2 Mouse)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R7

Plasmid

#111039

Purpose

Plasmid expresses the talin2 rod domain R7 a 5 helix bundle.

Depositor

Ben Goult

Article

Bouchet et al Elife. 2016 Jul 13;5. doi: 10.7554/eLife

Insert

TLN2 (Tln2 Mouse)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCML 221

Plasmid

#98587

Purpose

pMS2-CYFP. Expresses MS2 protein-CYFP fusion in E. coli for TriFC assay (kanR).

Depositor

Lydia Contreras

Article

Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

Insert

MS2

Use

Tags

Expression

Mutation

Promoter

pLacO

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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nucHCB1

Plasmid

#97424

Purpose

Nuclear Huntingtin Chromobody 1: "Happ1" intrabody recognizing amino acids 41–81 of huntingtin, fused to eYFP and nuclear localization signal.

Depositor

Ray Truant

Article

Maiuri et al Hum Mol Genet. 2016 Dec 25. pii: ddw395.

Insert

nucHCB1

Use

Tags

NLS and YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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LGhG-pHG165a

Plasmid

#90048

Purpose

Constitutive expression of a chimeric LacI:GalR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Tungtur et al J Mol Biol. 2010 Jan 29;395(4):785-802.

Insert

LGhG

Use

Tags

Expression

Bacterial

Mutation

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup88-XbaI

Plasmid

#87328

Purpose

To express shRNA against human Nup88

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup88

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-CMV.Pro

Plasmid

#79486

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5), CMV promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

CMV Promoter

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

CMV Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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GST-PC-1-CTS

Plasmid

#86067

Purpose

A bacterial expression plasmid encoding polycystin-1 ciliary targeting signal with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Polycystin-1 (PKD1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

contains the region from 4297-4303

Promoter

tac

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup93-XhoI

Plasmid

#87330

Purpose

To express shRNA against human Nup93

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup93

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pQCXIP-Laforin Myc 3CS (C250S,C266S,C278S)

Plasmid

#84661

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Nov. 24, 2016

Availability

Academic Institutions and Nonprofits only