Skip to main content
Addgene
Showing: 441 - 460 of 7274 results

  1. pACYC Destination

    Plasmid
    #207675
    Purpose
    Trio “destination vectors” which harbor an operon for constitutive expression of the repressors for each inducible promoter (CymR, PhlF, and VanR for cumate, DAPG and vanillate, respectively).
    Depositor
    Ramon Gonzalez
    Article
    Lee et al Metab Eng. 2024 Mar;82:262-273. doi: 10.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    CymRC, PhlF, VanCC
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    207675-plasmid-map-sequence-id-421910

    Enlarge

  8. pACT2-μ1A

    Plasmid
    #198168
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1A fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:
    Insert
    AP-1 μ1A
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    198168-plasmid-map-sequence-id-387525

    Enlarge

  9. Epac-SH226

    Plasmid
    #191448
    Purpose
    expression of cAMP sensor in mammalian cells
    Depositor
    Kees Jalink
    Insert
    mT2Del-EPACdDEPCD-tdTomato
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    191448-plasmid-map-sequence-id-382898

    Enlarge

  15. pAct-GST-H3

    Plasmid
    #182842
    Purpose
    A PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.
    Depositor
    Peter Harte
    Article
    Tie et al Development. 2009 Sep;136(18):3131-41. d
    Insert
    GST-histone H3 fusion
    Use
    Tags
    GST
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    182842-plasmid-map-sequence-id-360059

    Enlarge

  16. pAc5.1B-lambdaN_A

    Plasmid
    #145850
    Purpose
    Insect Expression of lambdaN
    Depositor
    Elisa Izaurralde
    Insert
    lambdaN
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    145850-plasmid-map-sequence-id-288295

    Enlarge

  17. pMC6-spacer

    Plasmid
    #176178
    Purpose
    yeast MoClo level-0 part plasmid type 6 containing non-coding DNA spacer for construction of MoClo plasmids without yeast marker
    Depositor
    Florian David
    Article
    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-
    Insert
    41 bp non-coding DNA spacer from pYTK048
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    176178-plasmid-map-sequence-id-353641

    Enlarge

  19. Epac-S-H171

    Plasmid
    #170353
    Purpose
    mTurq2Del_EPACdDEPCD_Q270E_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    mTurq2Del_EPACdDEPCD_Q270E_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170353-plasmid-map-sequence-id-345820

    Enlarge

  20. Epac-S-H170

    Plasmid
    #170352
    Purpose
    mTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    mTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170352-plasmid-map-sequence-id-345817

    Enlarge

Showing: 441 - 460 of 7274 results