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N112 nLV EF-1a-PGK-Puro Gal4-HP1a CS

Plasmid

#43969

Depositor

Jerry Crabtree

Article

Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

Insert

Cbx5 (Cbx5 Mouse)

Use

Lentiviral

Tags

Gal4/DBD

Expression

Mutation

N-term chromo domain removed

Promoter

EF-1a

Available since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Puro-EF-1α>TurboRFP(ns):T2A:{H2B-RFP}

Plasmid

#201120

Purpose

RFP expression in cells under EF-1α promoter; Histone-bound, nuclear

Depositor

Ori Bar-Nur

Article

Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

Insert

TurboRFP; H2B-RFP

Use

Lentiviral

Tags

Expression

Mutation

No

Promoter

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pEX-CMV-SP-YFP-STM2(15-746) EF hand D80A

Plasmid

#18864

Depositor

Tobias Meyer

Article

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

Stromal interaction molecule 2 (STIM2 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

EF hand mutant: D80A. YFP inserted between signal…

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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N163 nLV EF-1a-Hp1a(CS)-Frbx2(Frb+FrbWobb)-V5-PGK-Puro

Plasmid

#44195

Depositor

Jerry Crabtree

Article

Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

Insert

Cbx5 (Cbx5 Mouse)

Use

Lentiviral

Tags

Frb, Hu Frb x2(WT Frb+codon Wobble Frb), and V5/e…

Expression

Mutation

N-term chromo domain removed

Promoter

EF-1a

Available since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1s

Plasmid

#72484

Purpose

Express gene of interest under truncated EF-1 promoter

Depositor

Kazuhiro Oka

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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N205 nLV EF-1a-ZFHD1-link-FKBP-HA <T2A> HP1aCS-Frbx2-V5-PGK-Blast

Plasmid

#44017

Depositor

Jerry Crabtree

Article

Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

Inserts

FKBP
Cbx5 (Cbx5 Mouse)

Use

Lentiviral

Tags

Frb, HA, Hu Frb x2(WT Frb+codon Wobble Frb), V5/e…

Expression

Mutation

N-term chromo domain removed

Promoter

EF-1a

Available since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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pEX-CMV-SP-YFP-STM1(24-685) EF hand 3 mut (A79K, N80D, D82G)

Plasmid

#18865

Depositor

Tobias Meyer

Article

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

Stromal interaction molecule 1 (STIM1 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

3 mutations (A79K, N80D, D82G) in the EF hand of …

Promoter

Available since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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pEX-CMV-SP-YFP-STM2(15-746AA)-EF hand 3 mut (K83A,D84N,G86D)

Plasmid

#20174

Depositor

Tobias Meyer

Article

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

pEX-CMV-SP-YFP-STM2(15-746AA)-EF hand 3 mut (K83A,D84N,G86D) (STIM2 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

3 mutations (K83A,D84N,G86D) in the EF hand of ST…

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-Luc2-P2A-copGFP

Plasmid

#72485

Purpose

Express Luc2 and copGFP

Depositor

Kazuhiro Oka

Insert

Luc2-P2A-copGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1

Available since

Jan. 11, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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TTR C10S S85C K80D

Plasmid

#184743

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Use

Tags

Expression

Bacterial

Mutation

C10S, S85C, K80D

Promoter

T7

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C G83R

Plasmid

#184747

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Use

Tags

Expression

Bacterial

Mutation

C10S, S85C, G83R

Promoter

T7

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C Y78F

Plasmid

#184746

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Use

Tags

Expression

Bacterial

Mutation

C10S, S85C, Y78F

Promoter

T7

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TTR C10S S85C A81T

Plasmid

#184745

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Use

Tags

Expression

Bacterial

Mutation

C10S, S85C, A81T

Promoter

T7

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TTR C10S S85C K80E

Plasmid

#184744

Purpose

bacterial expression of huamn TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Use

Tags

Expression

Bacterial

Mutation

C10S, S85C, K80E

Promoter

T7

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TTR C10S S85C I84S

Plasmid

#184748

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Use

Tags

Expression

Bacterial

Mutation

C10S, S85C, I84S

Promoter

T7

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-EF1-Luc2-P2A-tdTomato

Plasmid

#72486

Purpose

Express Luc2 and tdTomato under EF1 promoter

Depositor

Kazuhiro Oka

Insert

Luc2-P2A-tdTomato

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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mEmerald EFHC2-N-18

Plasmid

#54077

Purpose

Localization: EF-Hand Domain , Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

EFHC2 (EFHC2 Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pAAV-EF1α-DIO-GtACR2-P2A-Voltron2ST-W3SL

Plasmid

#217676

Purpose

AAV-mediated, Cre-dependent co-expression of GtACR2 and soma-targeted Voltron2 (ORCHID) for assessing inhibitory receptor driving force through voltage imaging with concurrent activation of GtACR2.

Depositor

Joseph Raimondo

Article

Selfe et al bioRxiv 2023.08.30.555464

Insert

GtACR2-P2A-Voltron2ST

Use

AAV

Tags

Soma targeting sequence (Kv2.1) on Voltron2 gene …

Expression

Mammalian

Mutation

Promoter

EF‐1α

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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EF.GFP

Plasmid

#17616

Depositor

Linzhao Cheng

Article

Cui et al Blood. 2002 Jan 15. 99(2):399-408.

Insert

EF-1a promoter (EEF1A1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

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pDONR221-MICU1-mEF

Plasmid

#31765

Depositor

Vamsi Mootha

Article

Perocchi et al Nature. 2010 Sep 16;467(7313):291-6. Epu

Insert

MICU1 (MICU1 Human)

Use

Pdonr gateway

Tags

None

Expression

Mutation

Mutated in both EF-hand domains: D231A, E242K, D4…

Promoter

Available since

May 18, 2012

Availability

Academic Institutions and Nonprofits only

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