We narrowed to 4,553 results for: pcr

1. pCRB DRT7VTPLux*500YFP

   Plasmid

   #107961

   Purpose

   YFP half-circuit controller plasmid [ca. 558.5 relative RBS strength at PLas81*]

   Depositor

   Jim Haseloff

   Article

   Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

   Insert

   T7RNAP (modified)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCRB DRT7VTPLux*500CFP

   Plasmid

   #107960

   Purpose

   CFP half-circuit controller plasmid [ca. 558.5 relative RBS strength at PLas81*]

   Depositor

   Jim Haseloff

   Article

   Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

   Insert

   T7RNAP (modified)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCRB DRT7VTPLux*250

   Plasmid

   #107962

   Purpose

   Controller plasmid [ca. 249.0 relative RBS strength at PLas81*]

   Depositor

   Jim Haseloff

   Article

   Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

   Insert

   T7RNAP (modified)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCRB DRT7VTPLux*100

   Plasmid

   #107963

   Purpose

   Controller plasmid [ca. 110.5 relative RBS strength at PLas81*]

   Depositor

   Jim Haseloff

   Article

   Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

   Insert

   T7RNAP (modified)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCR2.1-INS-904

   Plasmid

   #53970

   Purpose

   904 bp insert from human INS gene used as a control for methylation-specific PCR assays

   Depositor

   Kevin Ferreri

   Article

   Husseiny et al PLoS One. 2012;7(10):e47942. doi: 10.137

   Insert

   human INS gene partial (INS Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCRISPaint-myc-PuroR

   Plasmid

   #80961

   Purpose

   CRISPaint gene tagging

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   myc tag
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   Promoter

   none

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCR8/GW/TOPO GFP

   Plasmid

   #69798

   Purpose

   Gateway entry vector for GFP

   Depositor

   Gerard Cagney

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pBS532 promoterless GFPcre

   Plasmid

   #11958

   Depositor

   Brian Sauer

   Article

   Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

   Insert

   GFP-cre (cre bacteriophage P1)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pCRII-Topo Btg1

   Plasmid

   #45865

   Depositor

   Jeffrey Macklis

   Article

   Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

   Insert

   Btg1 in situ probe (Btg1 Mouse)
   

   Use

   In situ

   Tags

   Expression

   Mutation

   contains bp# 1004-1638 of BC006834.1

   Promoter

   Available Since

   July 9, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pCR8-CHD5-FRAGMENT

    Plasmid

    #68879

    Purpose

    GW entry vector for CHD5

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    331-672aa

    Promoter

    none

    Available Since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pCRII-Topo Semcap3

    Plasmid

    #45874

    Depositor

    Jeffrey Macklis

    Insert

    Semcap3 in situ probe (Pdzrn3 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    contains bp# 3184-3805 of AF127085.1

    Promoter

    Available Since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pCR8-DJ1-D149A

    Plasmid

    #29355

    Depositor

    Mark Cookson

    Article

    Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

    Insert

    DJ1 (PARK7 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Asp149Ala

    Promoter

    Available Since

    March 31, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pCRII-Topo Inhba

    Plasmid

    #45868

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

    Insert

    Inhba in situ probe (Inhba Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    nt 428-933 from Inhba mRNA (NM_008380.1)

    Promoter

    Available Since

    June 11, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pCRISPEY-Z3-Nat-ADE2

    Plasmid

    #232106

    Purpose

    Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    Z3

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRISPEY-Z3-Hyg-ADE2

    Plasmid

    #232107

    Purpose

    Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    Z3

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCRISPR-cBEST-v2-SF14P

    Plasmid

    #209448

    Purpose

    improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the SF14P promoter

    Depositor

    Tilmann Weber

    Article

    Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

    Insert

    Codon optimized APOBEC1-nCas9-UGI fusion protein
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PtipA; sgRNA expression from SF14P promoter

    Available Since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCRISPR-cBEST-v2-kasOP*

    Plasmid

    #209447

    Purpose

    improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoter

    Depositor

    Tilmann Weber

    Article

    Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

    Insert

    Codon optimized APOBEC1-nCas9-UGI fusion protein
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PtipA; sgRNA expression from kasOP* promoter

    Available Since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCRISPR/eCas9-GPIDAF

    Plasmid

    #213710

    Purpose

    To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.

    Depositor

    Qilai Huang

    Article

    Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    3XFLAG and Twin-strep-tag

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCRISPR/eCas9-GPIBY55

    Plasmid

    #213709

    Purpose

    To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.

    Depositor

    Qilai Huang

    Article

    Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    3XFLAG and Twin-strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PCRbluntII-pHES7-STOPgRNA

    Plasmid

    #204349

    Purpose

    gRNA to target pig HES7 stop codon

    Depositor

    Li-Fang Chu

    Article

    Conrad et al Stem Cell Reports. 2023 Dec 12;18(12):23

    Insert

    Pig HES7-STOP-gRNA (HES7 Sus domesticus (pig))
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge