We narrowed to 1,772 results for: rpe

1. pEC2735 (pSpy0C2)

   Plasmid

   #220279

   Purpose

   integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombination

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. hTMEM115-TEV-3xHA-P2A-mScarlet HDR template

   Plasmid

   #214998

   Purpose

   Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   hTMEM115-TEV-3xHA-P2A-mScarlet HDR template (TMEM115 Human)
   

   Use

   Hdr template

   Tags

   3xHA

   Expression

   Mutation

   Promoter

   None

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template

   Plasmid

   #214999

   Purpose

   Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mNG for cell sorting.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template (TMEM115 Human)
   

   Use

   Hdr template

   Tags

   3xHA

   Expression

   Mutation

   Promoter

   None

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE pGK hNLRP3-mNG

   Plasmid

   #218643

   Purpose

   Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak pGK promoter with blast selection

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (NLRP3 Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   pGK

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221 hNLRP3-mNG-STOP

   Plasmid

   #218644

   Purpose

   Gateway vector for shuttling NLRP3 C-terminally tagged with mNeonGreen to different destination vectors (start and stop codon)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (NLRP3 Human)
   

   Use

   Gateway entry

   Tags

   mNeonGreen

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pH6Z-806

   Plasmid

   #85255

   Purpose

   Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

   Depositor

   Philip Pellett

   Article

   Lindquester et al Arch Virol. 1996;141(2):367-79.

   Insert

   fragment of the human herpesvirus 6B strain Z29 genome
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ZE38 pCS2P+ sp5l ORF

   Plasmid

   #17079

   Depositor

   Randall Moon

   Article

   Thorpe et al Development. 2005 Apr. 132(8):1763-72.

   Insert

   sp5l ORF (sp5l Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pH6Z-101

   Plasmid

   #85226

   Purpose

   Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

   Depositor

   Philip Pellett

   Article

   Lindquester et al Arch Virol. 1996;141(2):367-79.

   Insert

   fragment of the human herpesvirus 6B strain Z29 genome
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ZE34 Nlk1-pCS2+

   Plasmid

   #17075

   Depositor

   Randall Moon

   Article

   Thorpe et al Development. 2004 Jun;131(12):2899-909.

   Insert

   Nlk1 (nlk1 Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

10. ZE35 Nlk1myc-pCS2+

    Plasmid

    #17076

    Depositor

    Randall Moon

    Article

    Thorpe et al Development. 2004 Jun;131(12):2899-909.

    Insert

    Nlk1 (nlk1 Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    myc

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

11. ZE36 Nlk1(C387Y)-pCS2+

    Plasmid

    #17077

    Depositor

    Randall Moon

    Article

    Thorpe et al Development. 2004 Jun;131(12):2899-909.

    Insert

    Nlk1 (nlk1 Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    C387Y. Inactive mutant of NLK1.

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pHAGE-TMEM9-EGFP

    Plasmid

    #225515

    Purpose

    Lentiviral TMEM9-EGFP expression vector

    Depositor

    Wade Harper

    Article

    Gonzalez-Lozano et al bioRxiv [Preprint]. 2025 Feb 9:2025.02.0

    Insert

    TMEM9 (TMEM9 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEC2796 (pSpy0C4)

    Plasmid

    #220280

    Purpose

    integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the sagB locus via homologous recombination

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. msFam160b1 (msFHIP2A) g1 lentiCRISPRv2-mCherry

    Plasmid

    #218654

    Purpose

    Knockout vector for mouse Fam160b1 (Fhip2A)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    Fam160b1 (Fhip2a Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR221 msNLRP3(ΔPYD, I125M-END) (STOP)

    Plasmid

    #218640

    Purpose

    Gateway shuttling vector for cloning N-terminal NLRP3(ΔPYD, I125M-END) tags (contains a stop codon)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    delPYD (I125M-END)

    Promoter

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221 hNLRP3 R262W (disease-associated mutation)

    Plasmid

    #218647

    Purpose

    Gateway shuttling vector for cloning NLRP3-R262W (no stop codon)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (NLRP3 Human)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    R262W

    Promoter

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR221 msNLRP3(ΔPYD, I125M-END)

    Plasmid

    #218641

    Purpose

    Gateway shuttling vector for cloning N- and C-terminal NLRP3(ΔPYD, I125M-END) tags (contains no stop codon)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    delPYD (I125M-END)

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. msFam160b1 (msFHIP2A) g2 lentiCRISPRv2 mCherry

    Plasmid

    #218655

    Purpose

    Knockout vector for mouse Fam160b1 (Fhip2A)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    Fam160b1 (Fhip2a Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV UBC msNLRP3(LRRm5)-APEX2-3xFLAG-IRES-eGFP (H781E_Q782R_F785A)

    Plasmid

    #218636

    Purpose

    Proximity biotinylation of msNLRP3(LRRm5, H781E_Q782R_F785A)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Lentiviral

    Tags

    APEX2-3xFLAG

    Expression

    Mammalian

    Mutation

    H781E, Q782R, F785A

    Promoter

    UBC

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTwist Amp msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template

    Plasmid

    #215000

    Purpose

    Endogenously tag mouse TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template (Tmem115 Mouse)
    

    Use

    Hdr template

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    None

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge