We narrowed to 23,150 results for: CRISPR

1. pGTag Hygro EGFP nLAP +0

   Plasmid

   #194305

   Purpose

   CRISPR/Cas9-mediated generic protein tagging

   Depositor

   Frank Schnütgen

   Article

   Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

   Insert

   Hygro-P2A-nLAP(EGFP)
   

   Use

   Bacterial cloning vector

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBRC1

   Plasmid

   #183064

   Purpose

   Base editing plasmid for Pseudomonas chlororaphis

   Depositor

   Xuehong Zhang

   Article

   Yue et al Microb Biotechnol. 2022 Sep;15(9):2324-2

   Insert

   eSpCas9ppD10A
   

   Use

   CRISPR and Synthetic Biology

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMH183

   Plasmid

   #122856

   Purpose

   MS2-modified guide RNA targeting the FT promoter with GreenGate D-E flanking sequences

   Depositor

   Markus Schmid

   Article

   Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

   Insert

   MS2-modified sgRNA targeting the Arabidopsis FT promoter
   

   Use

   Golden gate compatible cloning vector

   Available Since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. OA-1050E (Ubiq-CasRx)

   Plasmid

   #132416

   Purpose

   Express CasRx under Ubiquitin-63E promoter

   Depositor

   Omar Akbari

   Article

   Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

   Insert

   CasRx
   

   Expression

   Insect

   Promoter

   Ubiq

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

   Plasmid

   #174140

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

   Promoter

   CMV and T7

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX459_gRNA pAS single_hspCas9

   Plasmid

   #193311

   Purpose

   Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   not applicable

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cas12c-sgRNA_BbsI_CAM

   Plasmid

   #183221

   Purpose

   Cas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   Cas12c single guide RNA containing BbsI sites in spacer sequence
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. bbCas9pluspAAA

   Plasmid

   #82581

   Purpose

   The plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNA

   Depositor

   Ivo Huijbers

   Article

   Pritchard et al Methods Mol Biol. 2017;1642:21-35. doi:

   Insert

   Cas9
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRTS-GBP-dCas9-mRFP

   Plasmid

   #102934

   Purpose

   Tetracyclin inducible expression construct for dCas9 fused to GFP-binding Protein (N-terminally) and mRFP (C-terminus)

   Depositor

   Sebastian Bultmann

   Article

   Anton et al Nucleus. 2017 May 4;8(3):279-286. doi: 1

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   GBP (GFP binding protein and mRFP

   Expression

   Mammalian

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM

    Plasmid

    #183077

    Purpose

    Plasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c tracrRNA and crRNA ending with a HDV ribozyme
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfB8792

    Plasmid

    #126910

    Purpose

    Template for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 gene

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    ADE2 gRNA
    

    Use

    Synthetic Biology

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pICSL1P2_crU626

    Plasmid

    #191777

    Purpose

    Cas12a guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICSL1P7_crU626

    Plasmid

    #191782

    Purpose

    Cas12a guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ147

    Plasmid

    #69299

    Purpose

    Golden Gate recipient and Gateway entry vector; assembly of 7 gRNAs

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiguide-puro-wt-ltr

    Plasmid

    #182635

    Purpose

    Expresses guide RNA, contains intact LTR

    Depositor

    Jakob Reiser

    Article

    Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

    Insert

    S. pyogenes sgRNA cassette
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pICSL1P6_crU626

    Plasmid

    #191781

    Purpose

    Cas12a guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pICSL1P3_crU626

    Plasmid

    #191778

    Purpose

    Cas12a guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pICSL1P4_crU626

    Plasmid

    #191779

    Purpose

    Cas12a guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICSL1P5_crU626

    Plasmid

    #191780

    Purpose

    Cas12a guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSHS293

    Plasmid

    #87348

    Purpose

    D435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET construct

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/D435C/C574S/E945C

    Promoter

    T7

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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