We narrowed to 10,795 results for: HA

1. SPYtag-GFPnanobody

   Plasmid

   #214837

   Purpose

   This plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al bioRxiv 2024.01.21.576499.

   Insert

   SPYtag-GFPnanobody
   

   Use

   Tags

   His-TEV-SUMO-

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti CMV RasV12 Neo (w108-1)

   Plasmid

   #22259

   Depositor

   Eric Campeau

   Insert

   HRAS G12V (HRAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   G12V mutant

   Promoter

   Available since

   Jan. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Cys-60nmSAH-SPYcatcher

   Plasmid

   #214840

   Purpose

   This plasmid encodes a protein (Cys-60nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al bioRxiv 2024.01.21.576499.

   Insert

   Cys-60nmSAH-SPYcatcher
   

   Use

   Tags

   His-TEV-SUMO-

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Cys-3HB-SPYcatcher

   Plasmid

   #214838

   Purpose

   This plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al bioRxiv 2024.01.21.576499.

   Insert

   Cys-3HB-SPYcatcher
   

   Use

   Tags

   His-TEV-SUMO-

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SPYtag-Histag-Avidin

   Plasmid

   #214836

   Purpose

   This plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al bioRxiv 2024.01.21.576499.

   Insert

   SPYtag-Histag-Avidin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Non tagged Avidin

   Plasmid

   #214835

   Purpose

   This plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al bioRxiv 2024.01.21.576499.

   Insert

   Non tagged Avidin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Cys-30nmSAH-SPYcatcher

   Plasmid

   #214839

   Purpose

   This plasmid encodes a protein (Cys-30nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al bioRxiv 2024.01.21.576499.

   Insert

   Cys-30nmSAH-SPYcatcher
   

   Use

   Tags

   His-TEV-SUMO-

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mApple-Farnesyl-5

   Plasmid

   #54899

   Purpose

   Localization: Plasma Membrane, Excitation: 568, Emission: 592

   Depositor

   Michael Davidson

   Insert

   Farnesyl (HRAS Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   encodes final 20 aa of NM_001130442.1

   Promoter

   CMV

   Available since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

9. GFP-rGBD

   Plasmid

   #26732

   Depositor

   William Bement

   Article

   Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

   Insert

   rhotekin (rtkn2 Mouse)
   

   Use

   Reporter for rho

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   RhoA binding domain of rhotekin

   Promoter

   Available since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MSCV H-Ras V12 IRES GFP

    Plasmid

    #18780

    Depositor

    Scott Lowe

    Insert

    H-Ras V12 (HRAS Human)
    

    Use

    Retroviral

    Tags

    IRES GFP

    Expression

    Mammalian

    Mutation

    V12

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti CMV/TO RasV12 Puro (w119-1)

    Plasmid

    #22262

    Depositor

    Eric Campeau

    Insert

    HRAS G12V (HRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    G12V mutant

    Promoter

    Available since

    Dec. 15, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PD14

    Plasmid

    #164529

    Purpose

    Expresses HA-Ub

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Ubiquitin
    

    Use

    Lentiviral

    Tags

    HA and Ub

    Expression

    Mutation

    WT

    Promoter

    CMV

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPB_EF1a_HA-tNGFR_PuroR

    Plasmid

    #212652

    Purpose

    piggyBac vector to express HA-tNGFR with puromycin resistance

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    tNGFR
    

    Use

    Piggybac

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MSP955

    Plasmid

    #98784

    Purpose

    Accessory Plasmid for PACE

    Depositor

    David Liu

    Article

    Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

    Insert

    proB-lysozyme-ggs-ENLYFQS-ggs-T7RNAP//T7pro-gIII-lux
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Dendra2-Farnesyl-5

    Plasmid

    #57717

    Purpose

    Localization: Membrane, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pBiFC-CC155

    Plasmid

    #22015

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    CC155
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Hs.HRAS

    Plasmid

    #83181

    Purpose

    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    HRAS (HRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mCherry-Farnesyl-5

    Plasmid

    #55045

    Purpose

    Localization: Plasma Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mEmerald-Farnesyl-5

    Plasmid

    #54093

    Purpose

    Localization: Membrane, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pFRT-DestFLAGHA_HuB

    Plasmid

    #65758

    Purpose

    constitutively expresses FLAG-HA-tagged HuB in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL2 (ELAVL2 Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only