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pCMV_SS-HA-V5-LacAnc100-CD4 TM_pDisplay

Plasmid

#245299

Purpose

expresses LaccID on the mammalian cell surface

Depositor

Alice Ting

Article

Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

Insert

LacAnc100-CD4TM

Expression

Yeast

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA ROSA 4b PhiC31 attP41

Plasmid

#242881

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human ROSA26 site

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for PhiC31 attP site

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA AAV 4a PhiC31 attP41

Plasmid

#242882

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human AAVS1 site

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for PhiC31 attP site

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA AAV 3b PhiC31 attP41

Plasmid

#242883

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human AAVS1 site

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for PhiC31 attP site

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA ROSA 4a PhiC31 attP41

Plasmid

#242880

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human ROSA26 site

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for PhiC31 attP site

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-RILPL1 (R293A)

Plasmid

#244981

Purpose

Express RILPL1 in mammalian cells with mCherry tag expressing a R293A mutation

Depositor

Luis Bonet-Ponce

Article

Bonet-Ponce et al J Cell Biol. 2025 Nov 3;224(11):e2024040

Insert

RILPL1 (RILPL1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

R293A

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3-APOX

Plasmid

#242344

Purpose

Expresses human APOX in cytosol

Depositor

Jeffrey Martell

Article

Fang et al ChemRxiv. 2025; doi:10.26434/chemrxiv-20

Insert

FLAG-APOX (APEX2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

F41V, S69I, Y235F, H239Y

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-DEST40-SIRT1

Plasmid

#242124

Purpose

Expression in mammalian cells

Depositor

J. Carl Barrett, Izumi Horikawa

Article

Michishita et al Mol Biol Cell. 2005 Oct;16(10):4623-35.

Insert

SIRT1 (SIRT1 Human)

Tags

V5-6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW10K_0x5

Plasmid

#177278

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Type

Empty backbone

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_Cox8-HaloTag7

Plasmid

#187077

Purpose

Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 in the mitochondria matrix

Depositor

Kai Johnsson

Article

Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

Insert

TOM20-dHaloTag7_T2A_Cox8-HaloTag7

Expression

Mammalian

Mutation

dHaloTag7 = HaloTag7-D106A

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pALN132

Plasmid

#239799

Purpose

Express mEGFP tagged mouse CENPT chimeric protein containing the rat CENPT histone fold domain

Depositor

Iain Cheeseman, Ally Nguyen

Article

Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

Insert

MmCENPT--Rn-Histone Fold Domain

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Promoter

Moloney Murine Leukemia Virus (MMLV) LTR promoter

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pGEMHE-MmCENP-W

Plasmid

#237433

Purpose

Expresses Mus musculus CENP-W; untagged; made for in vitro transcription

Depositor

Michael Lampson

Article

Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

Insert

Mus musculus CENP-W (Cenpw Mouse)

Expression

Mammalian

Promoter

T7

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGEMHE-MmCENPT-GFP

Plasmid

#237426

Purpose

Expresses Mus musculus CENP-T tagged with GFP at C-term; made for in vitro transcription (T7 promoter)

Depositor

Michael Lampson

Article

Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

Insert

Mus musculus CENP-T (Cenpt Mouse)

Tags

GFP

Expression

Mammalian

Promoter

T7

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pINDUCER20-Src(E378G)

Plasmid

#215466

Purpose

Dox-inducible lentiviral transfer vector with Src constitutively-active mutant (E378G)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Src (SRC Chicken)

Use

Lentiviral

Mutation

E378G (constitutively-active)

Promoter

mCMV, Doxycycline-inducible

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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EW209 PGK-ZNF35(5-8)-ZNF35(5-8)-deImmunLink-NZF(FLP-IN)

Plasmid

#236135

Purpose

Plasmid encoding the ZNF35/ZNF35 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoter

Depositor

Xiaojing Gao

Article

Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi

Insert

ZNF35(5-8)-ZNF35(5-8)-deImmunLink-NZF

Expression

Mammalian

Promoter

PGK

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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JPZF3 CMV-TO-SCN1A-117-NZF

Plasmid

#236164

Purpose

Plasmid encoding the SCN1A-117 zinc finger array attached by a GS linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sites

Depositor

Xiaojing Gao

Article

Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi

Insert

SCN1A-117-NZF

Expression

Mammalian

Promoter

CMV promoter with two TetR binding sites

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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JPZF10 CMV-TO-SCN1A-372-NZF

Plasmid

#236171

Purpose

Plasmid encoding the SCN1A-372 zinc finger array attached by a GS linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sites

Depositor

Xiaojing Gao

Article

Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi

Insert

SCN1A-372-NZF

Expression

Mammalian

Promoter

CMV promoter with two TetR binding sites

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_Z11 gpN:SpyTag

Plasmid

#225196

Purpose

Used for adding C-terminal SpyTag to the Plesiomonas ZOR0011 P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SpyTag

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS∆tum

Plasmid

#225198

Purpose

Used to create a markerless deletion of the E. coli HS tum gene

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

tum homology arms

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_lexA(Ind-)

Plasmid

#225199

Purpose

Used to create a non-inducible lexA by introducing a G85D mutation in E. coli HS

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

lexA homology arms

Expression

Bacterial

Mutation

lexA G85D

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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