We narrowed to 15,864 results for: cas9

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A3G5.13P-KE (spCas9)

Plasmid

#263232

Purpose

Mammalian expression of the A3G5.13P-KE (A3G5.13(R215K + R320E)-PAP) engineered base editor variant using spCas9. CMV promoter. AmpR marker for E. coli.

Depositor

Xue Gao

Publication

Zeng et al Mol Ther. 2026 Apr 1;34(4):2343-2360. do

Insert

A3G5.13P-KE (spCas9)

Use

CRISPR

Available since

Oct. 30, 2026

Availability

Academic Institutions and Nonprofits only

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A3G5.13P (spCas9)

Plasmid

#263230

Purpose

Mammalian expression of the A3G5.13P (A3G5.13-PAP) engineered base editor variant using spCas9. CMV promoter. AmpR marker for E. coli.

Depositor

Xue Gao

Publication

Zeng et al Mol Ther. 2026 Apr 1;34(4):2343-2360. do

Insert

A3G5.13P (spCas9)

Use

CRISPR

Available since

Oct. 27, 2026

Availability

Academic Institutions and Nonprofits only

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A3G5.13P-E (spCas9)

Plasmid

#263231

Purpose

Mammalian expression of the A3G5.13P-E (A3G5.13(R320E)-PAP) engineered base editor variant using spCas9. CMV promoter. AmpR marker for E. coli.

Depositor

Xue Gao

Publication

Zeng et al Mol Ther. 2026 Apr 1;34(4):2343-2360. do

Insert

A3G5.13P-E (spCas9)

Use

CRISPR

Available since

Oct. 27, 2026

Availability

Academic Institutions and Nonprofits only

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PRINCE-SpCas9-1

Plasmid

#251235

Purpose

PRINCE drug inducible gene editing system based on SpCas9 (Lentiviral-Part II: the SpCas9 expression is under drug-mediated control)

Depositor

Yu Wang

Publication

Zhang et al Sci Transl Med. 2026 May 27;18(851):eadx

Insert

EF1α-SpCas9; 2×NES; 2×ERT2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

July 2, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx2_WT_GFP (ABE8e_L34W_nCas9_WT)

Plasmid

#251549

Purpose

Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the L34W mutation in the deaminase domain

Depositor

George Church

Publication

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_L34W-nCas9_WT

Use

CRISPR

Expression

Mammalian

Mutation

ABEx2 L34W

Available since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx2_SpRY (ABE8e_L34W_nCas9_SpRY)

Plasmid

#251545

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY adenine base editor (ABE8e) carrying the L34W mutation in the deaminase domain

Depositor

George Church

Publication

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_L34W-nCas9_SpRY

Use

CRISPR

Expression

Mammalian

Mutation

ABEx2 L34W

Available since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx4_WT_GFP (ABE8e_V28C_M151E_nCas9_WT)

Plasmid

#251551

Purpose

Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the V28C and M151E mutations in the deaminase domain

Depositor

George Church

Publication

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_V28C_M151E-nCas9_WT

Use

CRISPR

Expression

Mammalian

Mutation

ABEx4 V28C_M151E

Available since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx3_WT_GFP (ABE8e_M151E_nCas9_WT)

Plasmid

#251550

Purpose

Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the M151E mutation in the deaminase domain

Depositor

George Church

Publication

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_M151E-nCas9_WT

Use

CRISPR

Expression

Mammalian

Mutation

ABEx3 M151E

Available since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-DNMT3A

Plasmid

#220237

Purpose

Expresses dCAS9-DNMT3A fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Publication

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-DNMT3A (dCas9-3A)
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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dCas9-KYP (M6)

Plasmid

#239480

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.

Depositor

Jian-Kang Zhu

Publication

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-dCas9-2XNLS-XTEN16-BFP-KYP

Use

CRISPR

Expression

Plant

Available since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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EML8357_pA3A-CBECas9

Plasmid

#234352

Purpose

A base editor derived by egg-cell specific promoter

Depositor

Erh-Min Lai

Publication

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pHS-dCas9

Plasmid

#240212

Purpose

Mammalian vector for transient expression of dCas9

Depositor

Wilfred Chen

Publication

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

dCas9

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

PGK

Available since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pdCas9-tdTomato

Plasmid

#232631

Purpose

catalytically inactive dCas9 for localization; labeled with tdTomato

Depositor

Karsten Rippe

Insert

dCas9-tdTomato

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SpCas9-miniCGBE1

Plasmid

#198548

Purpose

Expresses human codon-optimized SpCas9-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-miniCGBE1-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Publication

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-miniCGBE1

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-NRRH

Plasmid

#198540

Purpose

Expresses human codon-optimized SpCas9-NRRH and blasticidin resistance: EFS promoter-SpCas9-NRRH-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Publication

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NRRH

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A695Q

Plasmid

#207368

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A695Q

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, K848A, Q926A, K1003A, R1060A, amino…

Promoter

CBh

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A926Q

Plasmid

#207370

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A926Q

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, K1003A, R1060A, amino…

Promoter

CBh

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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B-evoSpCas9-E526K

Plasmid

#207365

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-E526K

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

M495V, Y515N, R661Q, amino acids 1005-1013 replac…

Promoter

CBh

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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B-evoSpCas9-V495M

Plasmid

#207363

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-V495M

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Y515N, K526E, R661Q, amino acids 1005-1013 replac…

Promoter

CBh

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A1060R

Plasmid

#207372

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A1060R

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, Q926A, K1003A, amino …

Promoter

CBh

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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