We narrowed to 10,383 results for: plasmids 101

1. pETM6-T7-VHH-C9BoNT-A

   Plasmid

   #202490

   Purpose

   Expresses the nanobody C9 BoNT/A with T7 induction

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   nanobody C9 BoNT/A
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSEED-Moontag:HA

   Plasmid

   #219648

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Moontag and HAtag using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   MoonTag:HA SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUASTattB-degradALFA

   Plasmid

   #219663

   Purpose

   Permits Gal4-inducible expression of degradALFA, a fusion of anti-ALFANb to the F-Box of Slmb.

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   degradALFA
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Dm_λHA-FLuc100_CSC

   Plasmid

   #210592

   Purpose

   Expresses lambdaHA-optimal Firefly (CSC metric; Drosophila)

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Insert

   LambdaHA-optimal Firefly luciferase (Dm optimization, CSC metric)
   

   Use

   Tags

   lambda HA

   Expression

   Insect

   Mutation

   Promoter

   Ac5

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUB_OptλHAramp-FLuc100

   Plasmid

   #210577

   Purpose

   Expresses Optimal lambda HA-Optimal Firefly luciferase

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Insert

   Optimal lambda HA-Optimal Firefly luciferase
   

   Use

   Tags

   lambda HA

   Expression

   Mammalian

   Mutation

   Promoter

   UBC human

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUB_NonoptλHAramp-FLuc100

   Plasmid

   #210579

   Purpose

   Expresses Nonoptimal lambda HA-Optimal Firefly luciferase

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Insert

   Nonoptimal lambda HA-Optimal Firefly luciferase
   

   Use

   Lentiviral and Luciferase

   Tags

   lambda HA

   Expression

   Mammalian

   Mutation

   Promoter

   UBC human

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1_SM-FLuc100-pA

   Plasmid

   #210584

   Purpose

   Expresses SM(FLAG)-optimal Firefly luciferase - pA

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Insert

   Spaghetti monster (FLAG)-optimal FLuc
   

   Use

   Luciferase

   Tags

   Spaghetti monster (FLAG)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Dm_λHA-FLuc0_CSC

   Plasmid

   #210589

   Purpose

   Expresses lambdaHA-nonoptimal Firefly (CSC metric; Drosophila)

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Insert

   LambdaHA-nonoptimal Firefly luciferase (Dm optimization, CSC metric)
   

   Use

   Luciferase

   Tags

   lambda HA

   Expression

   Insect

   Mutation

   Promoter

   Ac5

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSEED-EYFP:HA

   Plasmid

   #219656

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in EYFP and HA tag using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   EYFP:HA SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC18-LucFF

    Plasmid

    #220496

    Purpose

    To generate substrates to monitor Ku overloading and its impact on transcription at the DNA ends vicinity.

    Depositor

    Sébastien Britton

    Article

    Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

    Insert

    Firefly Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    July 3, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. aCE89

    Plasmid

    #204494

    Purpose

    TREtight promoted WBeta and PhlF promoted PhiC31 without PEST tags

    Depositor

    Ron Weiss

    Article

    Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

    Inserts

    φC31 integrase
    WBeta recombinase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    PhIF and TREtight

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. aCE90

    Plasmid

    #204495

    Purpose

    TREtight promoted WBeta and PhlF promoted PhiC31 with PEST tags

    Depositor

    Ron Weiss

    Article

    Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

    Inserts

    phiC31 recombinase
    WBeta recombinase
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    PhIF and TREtight

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. IRE-miR21mutant-SunTag-TREAT

    Plasmid

    #196933

    Purpose

    SunTag cassette was added in the coding region in the miR21mutant -IRE-TREAT reporter.

    Depositor

    Jeffrey Chao

    Article

    Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

    Insert

    Renilla luciferase
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. IRE-let7-SunTag-TREAT

    Plasmid

    #196926

    Purpose

    SunTag cassette was added in the coding region in the let7-IRE-TREAT reporter

    Depositor

    Jeffrey Chao

    Article

    Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

    Insert

    Renilla luciferase
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. IRE-let7R-SunTag-TREAT

    Plasmid

    #196927

    Purpose

    SunTag cassette was added in the coding region in the let7R-IRE-TREAT reporter.

    Depositor

    Jeffrey Chao

    Article

    Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

    Insert

    Renilla luciferase
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBEL1874

    Plasmid

    #170423

    Purpose

    Genetic complementation plasmid of LetAB with LetB mutant I834N

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(I834N)

    Promoter

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBEL2343

    Plasmid

    #170424

    Purpose

    Genetic complementation plasmid of LetAB with LetB mutant L123N

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(L123N)

    Promoter

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBEL2344

    Plasmid

    #170425

    Purpose

    Genetic complementation plasmid of LetAB with LetB mutant L125N

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(L125N)

    Promoter

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBEL2345

    Plasmid

    #170426

    Purpose

    Genetic complementation plasmid of LetAB with LetB mutant V126N

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(V126N)

    Promoter

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBEL1863

    Plasmid

    #170412

    Purpose

    Genetic complementation plasmid of LetAB with LetB mutant A473N

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(A473N)

    Promoter

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge