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mCherry-FKBP-E2

Plasmid

#175243

Purpose

Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to Ypet

Depositor

Michael Rosen

Publication

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

UBE2I (UBE2I Human)

Expression

Bacterial

Promoter

T7

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu R126A

Plasmid

#129376

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu R126A

Expression

Bacterial

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu R126I

Plasmid

#129377

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu R126I

Expression

Bacterial

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu A81I

Plasmid

#129352

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu A81I

Expression

Bacterial

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu D89A

Plasmid

#129354

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu D89A

Expression

Bacterial

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu V90D

Plasmid

#129355

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu V90D

Expression

Bacterial

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu A81E

Plasmid

#129356

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu A81E

Expression

Bacterial

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu I88D

Plasmid

#129357

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu I88D

Expression

Bacterial

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pT2MPEF-NLS-QUE37C

Plasmid

#129326

Purpose

ATP measurement, nucleus, Tol2 transposon

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-37C

Expression

Mammalian

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only
pRabies-EBFP-tTAn

Plasmid

#172120

Purpose

Expression of EBFP and tTA-InteinN in Rabies virus infected cells

Depositor

Chun Xu

Publication

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

EBFP, tTAn-InteinN

Use

Rabies virus

Promoter

CMV

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRabies-EGFP-rtTAn

Plasmid

#172119

Purpose

Expression of EGFP and rtTA-InteinN in Rabies virus infected cells

Depositor

Chun Xu

Publication

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

EGFP, rtTAn-InteinN

Use

Rabies virus

Promoter

CMV

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_M

Plasmid

#162815

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_M

Depositor

Simon Elsaesser

Publication

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_M

Use

Piggybac

Expression

Mammalian

Mutation

CoV2_M(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_9b

Plasmid

#162816

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_9b

Depositor

Simon Elsaesser

Publication

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_9b

Use

Piggybac

Expression

Mammalian

Mutation

CoV2_9b(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF6

Plasmid

#162817

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF6

Depositor

Simon Elsaesser

Publication

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF6

Use

Piggybac

Expression

Mammalian

Mutation

CoV2_ORF6(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF7b

Plasmid

#162818

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF7b

Depositor

Simon Elsaesser

Publication

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF7b

Use

Piggybac

Expression

Mammalian

Mutation

CoV2_ORF7b(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF10

Plasmid

#162819

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF10

Depositor

Simon Elsaesser

Publication

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF10

Use

Piggybac

Expression

Mammalian

Mutation

CoV2_ORF10(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF9c/14

Plasmid

#162820

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF9c/14

Depositor

Simon Elsaesser

Publication

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF9c/14

Use

Piggybac

Expression

Mammalian

Mutation

CoV2_ORF9c/14(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b_alt

Plasmid

#162821

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b_alt

Depositor

Simon Elsaesser

Publication

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF3b_alt

Use

Piggybac

Expression

Mammalian

Mutation

CoV2_ORF3b_alt(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*SMIM38

Plasmid

#162808

Purpose

STELLA plasmid for amber suppression of N-terminally labelled SMIM38

Depositor

Simon Elsaesser

Publication

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)SMIM38

Use

Piggybac

Expression

Mammalian

Mutation

SMIM38 (Met1, Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pVW294

Plasmid

#139491

Purpose

Insertion of the pGRE2 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Publication

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pGRE2 24xPP7sl

Expression

Yeast

Mutation

WT

Promoter

pGRE2

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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