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pCFJ2560 - pEXP(mosTI, unc-119)[Peft-3 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Plasmid

#159825

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pEXP(mosTI, unc-119)[Peft-3 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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RGS-6xHis-LIN-29-pcDNA3.1-

Plasmid

#52515

Purpose

expresses C. elegans LIN-29 in mammalian cells with RGS-6xHis tag at N-terminus

Depositor

Adam Antebi

Publication

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Lin-29 b (lin-29 Nematode)

Tags

RGS-6xHis

Expression

Mammalian

Mutation

wt (changed coding sequence in 5' (5th codon…

Promoter

CMV

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pPB-scrambled-mCherry-CAAX

Plasmid

#228738

Purpose

Membrane-tethered mCherry scrambled shRNA targeting Miro1

Depositor

Cagla Eroglu

Publication

Rodriguez Salazar et al J Cell Biol. 2025 Oct 6;224(10):e2024101

Insert

Miro1 (Rhot1, Rhot1 Rat, Mouse)

Expression

Mammalian

Available since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-shMiro1-mCherry-CAAX

Plasmid

#228741

Purpose

Membrane-tethered mCherry shRNA targeting Miro1

Depositor

Cagla Eroglu

Publication

Rodriguez Salazar et al J Cell Biol. 2025 Oct 6;224(10):e2024101

Insert

Miro1 (Rhot1, Rhot1 Rat, Mouse)

Expression

Mammalian

Available since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_Vimentin-dHaloTag7_T2A_mEGFP

Plasmid

#187075

Purpose

Expression of dHaloTag7 located at the intermediate filaments of mammalian cells

Depositor

Kai Johnsson

Publication

Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

Insert

Vimentin-dHaloTag7

Expression

Mammalian

Mutation

dHaloTag7 = HaloTag7-D106A

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Vrg4-V5-APEX2-URA

Plasmid

#207071

Purpose

Expressing Vrg4-V5-APEX2. Marker for late Golgi/early endosomes.

Depositor

Zhiping Xie

Publication

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Vrg4

Tags

V5-APEX2

Expression

Yeast

Promoter

pVRG4

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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mApple-ERM

Plasmid

#217377

Purpose

ER marker

Depositor

Chi-Lun Chang

Publication

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

rat cb5 ERM

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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NFAST(low)-mApple-6xHp

Plasmid

#214412

Purpose

Lipid droplet localization of low-affinity NFAST fused to mApple-6xHp

Depositor

Chi-Lun Chang

Publication

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RspA-N

Tags

mApple-6xHp

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-ADRB2-mNG

Plasmid

#195145

Purpose

Image recycling cargo

Depositor

Gia Voeltz

Publication

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ADRB3 (ADRB3 Human)

Expression

Mammalian

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST40-CES2A-ΔC

Plasmid

#200554

Purpose

Mammalian expression of secreted mouse CES2A

Depositor

Jonathan Long

Publication

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2A delta C-terminal (Ces2a Mouse)

Tags

6xHis, Flag, and V5

Expression

Mammalian

Promoter

CMV promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_E114I (pBS0839)

Plasmid

#185274

Purpose

For the mammalian expression of the human protein APOE_HUMAN_E114I. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

APOE_HUMAN_E114I

Expression

Mammalian

Mutation

E114I

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNAO1_G-alpha

Plasmid

#110013

Purpose

Protein expression and purification of GNAO1_G-alpha

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAO1_G-alpha (GNAO1 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_ESR2_Hormone-recep

Plasmid

#109907

Purpose

Protein expression and purification of ESR2_Hormone-recep

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ESR2_Hormone-recep (ESR2 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_AR_Hormone-recep

Plasmid

#109847

Purpose

Protein expression and purification of AR_Hormone-recep

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

AR_Hormone-recep (AR Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pPB CAG Cdk6 R31C-IRES-EGFP

Plasmid

#153946

Purpose

piggyBac transposon vector with CAG promoter expressing Cdk6 (R31C mutant) and EGFP

Depositor

Luca Tiberi

Publication

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

Cdk6 (Cdk6 Mouse)

Expression

Mammalian

Mutation

R31C

Promoter

CAG

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Rab23

Plasmid

#31742

Depositor

Ron Vale

Publication

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab23 (Rab23 Fly)

Tags

GFP

Expression

Insect

Promoter

Actin

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pCS2 xLabial

Plasmid

#24965

Depositor

Edward De Robertis

Publication

Blumberg et al Science. 1991 Jul 12. 253(5016):194-6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

labial (hoxd1 Frog)

Expression

Mammalian

Available since

June 30, 2010

Availability

Academic Institutions and Nonprofits only
pART27

Plasmid

#247986

Purpose

Binary vector to receive a NotI fragment from pART7 with a gene to be stably expressed in plants via agrobacterium-mediated transformation.

Depositor

Andrew Gleave, Christoph Plieth

Publication

Gleave et al Plant Mol Biol. 1992 Dec;20(6):1203-7.

Type

Empty backbone

Expression

Plant

Available since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW mCherry-P2A-NeoR

Plasmid

#239570

Purpose

Cytosolic expression of mCherry

Depositor

Tim Ahfeldt, Joel Blanchard

Publication

et al iScience 28 (2025)

Insert

mCherry

Use

Lentiviral

Promoter

UbC

Available since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-BTC-ScNeo

Plasmid

#209898

Purpose

To monitor the status of Betacellulin, the plasmid encodes a recombinant BTC fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Publication

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

BTC-ScNeo (BTC Human)

Use

Lentiviral

Tags

mNeonGreen and mScarlet

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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