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pLKO-U6-Letm1shRNA-hPGK-miRFPnano

Plasmid

#215365

Purpose

Expresses a rat Letm1 specific shRNA and miRFPnano under a separate hPGK promoter

Depositor

Jaime de Juan-Sanz

Article

Amrapali Vishwanath et al Nat Metab. 2026 Feb 11. doi: 10.1038/s42

Insert

Letm1 shRNA Target sequence (Letm1 Rat)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-Camk2a(short)-mRFP1.Letm1[miR30-shRNA]-WPRE

Plasmid

#214404

Purpose

Expresses a mouse Letm1 specific microRNA in tandem with mRFP1

Depositor

Jaime de Juan-Sanz

Article

Amrapali Vishwanath et al Nat Metab. 2026 Feb 11. doi: 10.1038/s42

Insert

mRFP1 with Letm1 specific miRNA

Use

AAV

Expression

Mammalian

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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CamKII-mito4x-GCaMP6f-IRES2-MCS

Plasmid

#212660

Purpose

Expresses under a CamKII promoter: mito4x-GCaMP6f and a multiple cloning site (MCS) after an IRES sequence

Depositor

Jaime de Juan-Sanz

Article

Amrapali Vishwanath et al Nat Metab. 2026 Feb 11. doi: 10.1038/s42

Inserts

mito-4x-GCaMP6f
Letm1 EF-hand mutant (Letm1 Rat)

Use

Lentiviral

Expression

Mammalian

Mutation

D676A, D680A

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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CamKII-mito4x- GCaMP6f-IRES2-rat-Letm1-ΔEFhand

Plasmid

#212662

Purpose

Expresses under a CamKII promoter: mito4x-GCaMP6f and rat-Letm1 with mutations in its EF-hand

Depositor

Jaime de Juan-Sanz

Article

Amrapali Vishwanath et al Nat Metab. 2026 Feb 11. doi: 10.1038/s42

Inserts

mito-4x-GCaMP6f
Letm1 EF-hand mutant (Letm1 Rat)

Use

Lentiviral

Expression

Mammalian

Mutation

D676A, D680A

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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CamKII-mito4x-GCaMP6f-Drosophila-Letm1

Plasmid

#212663

Purpose

Expresses under a CamKII promoter: mito4x-GCaMP6f and Drosophila-Letm1

Depositor

Jaime de Juan-Sanz

Article

Amrapali Vishwanath et al Nat Metab. 2026 Feb 11. doi: 10.1038/s42

Inserts

mito-4x-GCaMP6f
Letm1 (Letm1 Fly)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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CKII-NPTX1-pH

Plasmid

#217974

Purpose

Expression of NPTX1-pHluorin for optical monitoring of presynaptic release in neurons

Depositor

Jaime de Juan-Sanz

Article

Pascual-Caro et al PLoS Biol. 2024 Oct 28;22(10):e3002860.

Insert

Neuronal Pentraxin 1-pHluorin

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Promoter

CaMKIIa

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-S473L-pHluorin

Plasmid

#185540

Purpose

Expresses mutant S473L LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed serine 473 for leucine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-Y433A-pHluorin

Plasmid

#185541

Purpose

Expresses mutant Y433A LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed tyrosine 433 for alanine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-R474Q-pHluorin

Plasmid

#185542

Purpose

Expresses mutant R474Q LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed arginine 474 for glutamine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-T380A-pHluorin

Plasmid

#185543

Purpose

Expresses mutant T380A LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed threonine 380 for alanine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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