We narrowed to 13,642 results for: NTS

1. pDONR221 NLRP1 P1214R

   Plasmid

   #166822

   Purpose

   Gateway pDONR vector encoding NLRP1 with the patient mutation P1214R (Grandemange et al., 2017) (no stop codon)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Expression

   Mutation

   P1214R

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMOD_B2121

   Plasmid

   #91065

   Purpose

   Module B, Promoter: GmUbi, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   GmUbi

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pInducer21-3xFlag-S208C-MEFV

   Plasmid

   #167019

   Purpose

   Lentiviral plasmid with 3xFlag-pyrin (S208C/PAAND variant) under the control of a doxycycline-inducible promoter

   Depositor

   Thomas Henry

   Article

   Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

   Insert

   MEFV (MEFV Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mutation

   Serine 208 changed to Cysteine

   Promoter

   CMV Dox inducible

   Available since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. CoV2-N-D377Y

   Plasmid

   #177957

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D377Y). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (D377Y) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D377Y

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. FB027

   Plasmid

   #119712

   Purpose

   Multipartite assembly obtained by combining FB parts FB026+FB003 into pDGB3omega1. Used for fluorescent tagging of fungi (Hyg resistance +YFP) according to FungalBraid modular DNA assembly for ATMT

   Depositor

   Jose Marcos

   Article

   Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

   Insert

   PgpdA::YFP::TtrpC(←)-PtrpC::hph::Ttub (→)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. QQR1 variant

   Plasmid

   #138564

   Purpose

   Expresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   QQR1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   G1218R, N1286Q, I1331F, D1332K, R1333Q, R1335Q, T…

   Promoter

   EFS

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. DsRed +9e

   Plasmid

   #191766

   Purpose

   Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e

   Depositor

   Daniel Gerlich

   Article

   Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

   Insert

   DsRed
   

   Use

   Tags

   N terminal fusion of positively charged polypetod…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMOD_C2516

   Plasmid

   #91084

   Purpose

   Module C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   At7SL

   Available since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. DCT.VV.Orf207

   Plasmid

   #12895

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf207
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   *

   Promoter

   Available since

   Dec. 29, 2006

   Availability

   Academic Institutions and Nonprofits only

10. KHBD00259

    Plasmid

    #34391

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG31240 (repo Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

11. p3A9cbetaTCR.20beta

    Plasmid

    #69592

    Purpose

    TCR beta chain genomic transgenic construct for creating OT-II mice

    Depositor

    Francis R. Carbone

    Article

    Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

    Insert

    TCR beta VJ region from clone 1.1 (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pLX-B3omega2 (GB1695)

    Plasmid

    #160644

    Purpose

    pLX series: pBBR1-based T-DNA binary vector for plant transformation (omega2)

    Depositor

    Juan Antonio García, Fabio Pasin

    Article

    Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX-B2alpha2 (GB1696)

    Plasmid

    #160620

    Purpose

    pLX series: pBBR1-based T-DNA binary vector for plant transformation (alpha2)

    Depositor

    Juan Antonio García, Fabio Pasin

    Article

    Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.2 mir-1-1 reporter hsa-mir-92a-1

    Plasmid

    #46672

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-mir-92a-1 (MIR92A1 Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDIRECT_22D

    Plasmid

    #91136

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A, H840A

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. CABE-T2.6

    Plasmid

    #193263

    Purpose

    Expression of CABE-T2.6 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CABE-T2.6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.2 mir-1-1 reporter hsa-mir-128-1

    Plasmid

    #46675

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-mir-128-1 (MIR128-1 Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. CBE-T1.6

    Plasmid

    #193275

    Purpose

    Expression of CBE-T1.6 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CBE-T1.6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWZL Blast H-Ras G12V E37G

    Plasmid

    #12277

    Depositor

    Bob Weinberg

    Article

    Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

    Insert

    H-Ras G12V E37G (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Contains G12V activating mutation. The E37G mutat…

    Promoter

    Available since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pCMV-MtPylRS (human-opti)

    Plasmid

    #164080

    Purpose

    To express a Methanosarcina thermophila derived PylRS in mammalian cells for genetic code expansion

    Depositor

    Tao Liu

    Article

    Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

    Insert

    MtPylRS-478(human-opti)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCMV-MfBulKRS (human-opti)

    Plasmid

    #164196

    Purpose

    To express a Methanosarcina flavescens derived BulKRS in mammalian cells for genetic code expansion

    Depositor

    Tao Liu

    Article

    Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

    Insert

    Mf BulKRS(human-opti)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Y269A;Y347F

    Promoter

    CMV

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pWZL Blast H-Ras G12V Y40C

    Plasmid

    #12279

    Depositor

    Bob Weinberg

    Article

    Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

    Insert

    H-Ras G12V Y40C (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Contains G12V activating mutation. The Y40C mutat…

    Promoter

    Available since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

23. pLV.U6.gLacZ.Cas9-T2A-GFP

    Plasmid

    #170361

    Purpose

    Negative control plasmid used for Crispr/cas9 based disruption.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

    Insert

    Cas9-T2A-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLenti-3xFLAG-Puro PAK5 K478R K469R

    Plasmid

    #167180

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R K469R

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R K469R
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. DCT.VV.Orf166

    Plasmid

    #12854

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf166
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

26. DCT.VV.Orf212

    Plasmid

    #12900

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf212
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

27. pDIRECT_21D

    Plasmid

    #91131

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A, H840A

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMM2-rPMCA3b

    Plasmid

    #47763

    Purpose

    mammalian expression of rat PMCA3b

    Depositor

    John Penniston, Emanuel Strehler

    Article

    Enyedi et al J Biol Chem. 1997 Oct 31;272(44):27525-8

    Insert

    PMCA3b (Atp2b3 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

29. pFAB3992

    Plasmid

    #47823

    Purpose

    BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD16 efficiency.

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    promoter 14 and BCD16
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    see insert

    Available since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMOD_B2519

    Plasmid

    #91076

    Purpose

    Module B, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    OsU3

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge