We narrowed to 6,824 results for: pcDNA3.1+
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Plasmid#163110PurposeExpression of mScarlet-I_H-NSdbd in eukaryotes for visualisation of nuclear DNADepositorInsertNLS_mScarlet-I_H-NSdbd_NLS
UseEukaryotic expressionExpressionMammalianPromoterCMV promoter; T7 promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
mGFAP-Halo
Plasmid#169474Purposethe host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sitesDepositorAvailable SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
POLArIS_NbVim-cp10GFP
Plasmid#171020PurposeExpresses NbVim-cp10GFP#2 in mammalian cellsDepositorInsertanti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)
Tagscp10GFPExpressionBacterial and MammalianPromoterCMV promoterAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-HF-tetra-com-vector
Plasmid#132555PurposeVector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.DepositorInsertsHigh fidelity Cas9
sgRNA scaffold with com replacing the Tetraloop
ExpressionMammalianAvailable SinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-PRMT3(E338Q)
Plasmid#164696PurposeExpression of PRMT3(E338Q) with an N-terminal FLAG tagDepositorAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector
Plasmid#132560PurposeVector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.DepositorInsertsABEmax
sgRNA, with Tetraloop replaced by com aptamer
ExpressionMammalianPromoterCMVAvailable SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PPO-Venus
Plasmid#139502PurposeExpresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.DepositorInsertparapinopsin
TagsVenus tag following 3x Ala linkerExpressionMammalianAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-CD28TMD-NTEVp(H75S)
Plasmid#137828PurposeMESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV proteaseDepositorInsertFKBP-CD28TMD-NTEVp(H75S)
Tags3x FLAGExpressionMammalianMutationMutated Histidine 75 to Serine in NTEVp fragmentPromoterCMVAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Integrin-αv Headpiece M400GC
Plasmid#159637PurposeContains integrin alpha v residues 1–594 with the M400C mutation and Gly inserted prior to residue 400DepositorInsertalpha v headpiece M400GC
Tags6x His and Strep IIExpressionMammalianMutationM400CAvailable SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-ABE-3'UTR-sgRNA-ST2-com vector
Plasmid#136270PurposeExpresses ABE and ST2-com modified sgRNA scaffoldDepositorInsertABE and sgRNA
UseCRISPR and LentiviralExpressionMammalianAvailable SinceNov. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEMAX-NLS-P2A_BLAST
Plasmid#135355PurposeExpression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistanceDepositorInsertABEMax-2XNLS
UseCRISPRExpressionMammalianMutationWTAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgO-sfGFP (TB40/E)
Plasmid#136451PurposeMammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) fused to superfolder GFPDepositorInsertglycoprotein O (UL74 Human betaherpesvirus 5)
TagsGly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …ExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgH (TB40/E)
Plasmid#136446PurposeMammalian expression plasmid for HCMV glycoprotein H (TB40/E strain), full-lengthDepositorInsertglycoprotein H (UL75 Human betaherpesvirus 5)
ExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2-WT(732)
Plasmid#154098PurposeMammalian expression plasmid for dimeric soluble ACE2 (untagged)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
ExpressionMammalianMutationExtracellular, soluble protease and neck domains …PromoterCMVAvailable SinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2-8his
Plasmid#149270PurposeMammalian expression plasmid for his-tagged sACE2 high affinity variant 2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsShort GSG linker and 8his tagExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-hCOL1A1
Plasmid#140112PurposeExpresses fluorescently-tagged human Type I procollagen α1 chain with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 (COL1A1 Human)
TagsmCeruleanExpressionMammalianMutationCOL1A1 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2-Fc(IgA1)
Plasmid#145157PurposeMammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsFc region of human IgA1ExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v3-sfGFP
Plasmid#145174PurposeMammalian expression plasmid for sACE2-sfGFP high affinity variant 3DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTE3646
Plasmid#137714PurposeExpresses LbCas12a RVRR variant in mammalian cells.DepositorInsertLbCas12a-RVRR
Tags3xHA, NLS, and SV40 NLSExpressionMammalianMutationG532R, K538V, Y542R, K595RPromoterCMVAvailable SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-RNF126 C231A
Plasmid#138648PurposeMammalian expression of full-length2 HA-tagged RNF126 with an inactivating mutation in the RING domain (C231A)DepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only