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pCJH026_proD_Cas12c-multiplexing_CAM

Plasmid

#183073

Purpose

Plasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repression

Depositor

Jennifer Doudna

Publication

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Expressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript

Use

CRISPR

Expression

Bacterial

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HCV-Fluc_AC

Plasmid

#148447

Purpose

Mammalian Expression of Fluc

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

Fluc

Expression

Mammalian

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-Rluc-6xbsMS2-random_AC

Plasmid

#148431

Purpose

Mammalian Expression of 6xbsMS2-random

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-random

Expression

Mammalian

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot2-del458-482-dsRNAres_U

Plasmid

#147716

Purpose

Insect Expression of DmNot2-del458-482-dsRNAres

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del458-482-dsRNAres (Rga Fly)

Expression

Insect

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot2-del393-429-W556E-dsRNAres_U

Plasmid

#147698

Purpose

Insect Expression of DmNot2-del393-429-W556E-dsRNAres

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del393-429-W556E-dsRNAres (Rga Fly)

Expression

Insect

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsRck_296-472-10xmut_AC

Plasmid

#148420

Purpose

Mammalian Expression of HsRck_296-472-10xmut

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck_296-472-10xmut (DDX6 Human)

Expression

Mammalian

Mutation

Q309A, H312A, T316A, R320A, Q322A, N324A, R335A, …

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1674-1962-dsRNAres_AC

Plasmid

#148402

Purpose

Insect Expression of DmNot1_1674-1962-dsRNAres

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmNot1_1674-1962-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

2 silent mutations compared to the sequence given…

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot3-del607-634-F673E_S

Plasmid

#147489

Purpose

Mammalian Expression of HsNot3-del607-634-F673E

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3-del607-634-F673E (CNOT3 Human)

Expression

Mammalian

Mutation

two silent mutations compared to the sequence giv…

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_1-101-V5His6_D

Plasmid

#146121

Purpose

Insect Expression of DmEDC3_1-101

Depositor

Elisa Izaurralde

Publication

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_1-101 (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_1-338-V5His6_D

Plasmid

#146122

Purpose

Insect Expression of DmEDC3_1-338

Depositor

Elisa Izaurralde

Publication

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_1-338 (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_1-440-V5His6_D

Plasmid

#146123

Purpose

Insect Expression of DmEDC3_1-440

Depositor

Elisa Izaurralde

Publication

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_1-440 (Edc3 Fly)

Expression

Insect

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_102-680_D

Plasmid

#146124

Purpose

Insect Expression of DmEDC3_102-680

Depositor

Elisa Izaurralde

Publication

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_102-680 (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_331-680_D

Plasmid

#146125

Purpose

Insect Expression of DmEDC3_331-680

Depositor

Elisa Izaurralde

Publication

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_331-680 (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLSm1_A

Plasmid

#145886

Purpose

Insect Expression of Dm-Lsm1

Depositor

Elisa Izaurralde

Publication

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

Dm-Lsm1

Expression

Insect

Mutation

Two silent mutation compared to the sequence give…

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLSm4_A

Plasmid

#145889

Purpose

Insect Expression of Dm-Lsm4

Depositor

Elisa Izaurralde

Publication

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

Dm-Lsm4

Expression

Insect

Mutation

one non silent mutation G127R compared to the seq…

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDH010

Plasmid

#157645

Purpose

sMHT-113 fused non-dimerizing GRX2-C37A mutant

Depositor

Jonathan Silberg

Publication

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-GRX2_C37A
GRX2_C37A-sMHT(114-230)

Expression

Bacterial

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pSV40L-TN

Plasmid

#165827

Purpose

Viral Simian virus 40 Late poly(a) terminator element Compatible with transposon 3' ITRs

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

SV40L

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGGSx4 CT

Plasmid

#165811

Purpose

Flexible peptide linker of 4 repeats of GGS, for assembly of C-terminal TL5 tags to TL4 parts

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

GGSx4 CT

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLexA BD

Plasmid

#165821

Purpose

LexA DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

LexA BD

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pQFBD

Plasmid

#165823

Purpose

QF DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

QFBD

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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