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Plasmid#225078PurposeExpresses precursor peptide and lanthipeptide synthetase from Chondromyces LanIV (FAST-RiPPs) in E. coliDepositorInsertLanA (LanIV-Chondromyces)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanI-101
Plasmid#225065PurposeExpresses precursor peptide, lanthipeptide cyclase, and lanthipeptide dehydratase from LanI-101 (FAST-RiPPs) in E. coliDepositorInsertLanA (LanI-101)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-a(+)-6xHis-mBlueberry(-9)
Plasmid#219416PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertmBlueberry2(-9)
UseTagsMGHHHHHGGExpressionBacterialMutationK137E, K199E, R234EPromoterT7Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL2del
Plasmid#216742PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 2 (OL2) by examining the variant lacking OL2 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 2 (OL2) with the sequence of [LEDSGYMA…PromoterAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-FKBP12-Sm
Plasmid#217004PurposeExpression of FKBP12-Linker-Small BiTDepositorInsertFKBP12-Linker-Small BiT
UseTagsSplit Nanoluciferase SmallBiTExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-FRB-Lg
Plasmid#216981PurposeExpression of FRB-Linker-Large BiTDepositorInsertFRB-Linker-Large BiT
UseTagsSplit Nanoluciferase LargeBiTExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-NIR-FbLAG30
Plasmid#220748PurposeNIR-FbLAG30 expression in bacteria.DepositorInsertNIR-FbLAG30
UseAffinity Reagent/ AntibodyTagsExpressionBacterialMutationFor bacterial cytoplasmic expression, the pelB si…PromoterT7Available sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyCatcher -AVI
Plasmid#223635PurposeProtein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsAVI tag and His6ExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
SUMO-LPQTG-pET
Plasmid#221851PurposeExpresses SUMO-2 with sortase motif and His tagDepositorInsertSUMO-2 (SUMO2 Human)
UseTagsExpressionBacterialMutationGln88Leu, Gln89ProPromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER-E169A
Plasmid#222190PurposeBacterial expression of mouse PTER-E169A proteinDepositorInsertPTER (Pter Mouse)
UseTags6xHis and StrepExpressionBacterialMutationE169APromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only