We narrowed to 9,127 results for: Pol

1. pLz78_CB_operon_CsoS2_delNTD

   Plasmid

   #140860

   Purpose

   Same as pLz37 but with CsoS2 truncated to remove the entire NTD (i.e. N1, N2, N3, and N4 are removed)

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CbbL, CbbS, CsoS2 (delNTD), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
   

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-235 of CsoS2

   Available Since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLz76_CB_operon_CsoS2_N4

   Plasmid

   #140858

   Purpose

   Same as pLz37 but with CsoS2 truncated such that NTD repeats N1, N2, and N3 are removed

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CbbL, CbbS, CsoS2 (delN1-N3), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
   

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-189 of CsoS2

   Available Since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBz109_CsoS2_NTD

   Plasmid

   #140842

   Purpose

   His-tagged CsoS2 N-terminal domain (NTD), pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CsoS2 N-terminal domain (NTD)
   

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 262-869

   Promoter

   T7

   Available Since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. sg1

   Plasmid

   #113966

   Purpose

   Single short guide RNA targeting GTATAGCATACATTATACG

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   sg1
   

   Promoter

   U6

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBz110_CsoS2_CTD

   Plasmid

   #140844

   Purpose

   His-tagged CsoS2 C-terminal domain (CTD), pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CsoS2 C-terminal domain (CTD)
   

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-594

   Promoter

   T7

   Available Since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKM136

   Plasmid

   #134177

   Purpose

   Bacterial expression of his-CENP-C 700-943

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Cell. 2014 Jul 17;158(2):397-411. doi: 1

   Insert

   his-CENP-C 700-943 (CENPC Human)
   

   Expression

   Bacterial

   Mutation

   aa 700-943

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR207-OEP7(1–50)

   Plasmid

   #100601

   Purpose

   Gateway entry vector for AtOEP7(1–50)

   Depositor

   Yutaka Kodama

   Article

   Tanaka et al J Plant Res. 2017 Jun 20. doi: 10.1007/s

   Insert

   OEP7(1–50) (OEP7 Mustard Weed)
   

   Use

   Gateway entry vector

   Available Since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pst39-NST-clone14

   Plasmid

   #133421

   Purpose

   S. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).

   Depositor

   Gerhard Wagner

   Insert

   NIP1/SUI1/TIF5 (NIP1, TIF5, SUI1 Budding Yeast)
   

   Expression

   Bacterial

   Mutation

   ORF for Nip1 encodes aa 4-156 (not complete ORF),…

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA-dabAB2

   Plasmid

   #133012

   Purpose

   pFA carrying the badabA2 (locus_tag: BAS2958) and badabB2 genes (locus_tag: BAS2959)

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Expression

   Bacterial

   Promoter

   tetR

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFE-dabAB2 C351A

    Plasmid

    #133005

    Purpose

    pFE carrying a dabA2 gene with a C351A mutation and a dabB2 gene (Uniprot: D0KWS8)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Expression

    Bacterial

    Mutation

    C351A

    Promoter

    tetR

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. YCe1885 HC_Kan_Kozak-ATG-MLS_p6

    Plasmid

    #100676

    Purpose

    part designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targeting

    Depositor

    Yizhi Cai

    Article

    Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

    Insert

    Kozak-ATG-MLS
    

    Use

    Synthetic Biology

    Available Since

    Nov. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. YCe2735 HC_Kan_Kozak-ATG-IgkL_p6

    Plasmid

    #100697

    Purpose

    part designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targeting

    Depositor

    Yizhi Cai

    Article

    Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

    Insert

    Kozak_ATG_IgkL
    

    Use

    Synthetic Biology

    Available Since

    Nov. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. CTL2 (M2225R)

    Plasmid

    #96952

    Purpose

    Expresses C-Terminal segment of C. elegans Piezo1 with M2225R mutation in E. coli

    Depositor

    Douglas Rees

    Article

    Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

    Insert

    CTL2 (M222R) of PIEZO1
    

    Tags

    6x Histidine Tag

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21a - MPX

    Plasmid

    #89619

    Purpose

    [Edit] Expresses recombinant 6HIS tagged T. cruzi mitochondrial tryparedoxin peroxidase protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Mitocondrial Tryparedoxin peroxidase
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21a - EtfA

    Plasmid

    #89616

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein A in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Electron transport flavoprotein alpha subunit
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. GST-PC-1-CTS

    Plasmid

    #86067

    Purpose

    A bacterial expression plasmid encoding polycystin-1 ciliary targeting signal with N-terminus GST-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Polycystin-1 (PKD1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains the region from 4297-4303

    Promoter

    tac

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21a - TDR

    Plasmid

    #89627

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi thiol dependent reductase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    thiol dependent reductase
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21a - NFDD

    Plasmid

    #89624

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi NAD-FAD–dependent dehydrogenase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    NAD/FAD dependent dehydrogenase
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBP-J23108

    Plasmid

    #72964

    Purpose

    Level 0 - J23108 standard iGEM promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    J23108 promoter
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBP-J23114

    Plasmid

    #72965

    Purpose

    Level 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    J23114 promoter
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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