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pAAV-EF1a-Flex-DN-AMPK-K45R-T2A-mCherry

Plasmid

#83491

Purpose

cre-dependent DN-AMPK under EF1a promoter

Depositor

Dong Kong

Article

Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

Insert

DN-AMPK-2A-mCherry

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

K45R

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-SpCas9_gRNA(RHO-P23H)-CMV-mTagBFP2_(RAS3613)

Plasmid

#228252

Purpose

pU6 (human U6) expression of SpCas9 sgRNA targeting RHO P23H mutation and pCMV expression of mTagBFP2

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

SpCas9 P23H sgRNA, mTagBFP2

Expression

Mammalian

Mutation

n/a

Promoter

CMV and U6

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-ChRmine-eYFP-Kv2.1-WPRE

Plasmid

#130997

Purpose

Double floxed soma-targeted ChRmine-eYFP under the control of Ef1a promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

EYFP-Kv2.1

Promoter

Ef1a

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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AiP1365 - pAAV-AiE2136m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230630

Purpose

AiE2136m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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AiP1376 - pAAV-AiE2147m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#220645

Purpose

AiE2147m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AiP1962 - pAAV-AiE2591m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214592

Purpose

AiE2591m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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AiP1425 - pAAV-AiE2132m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214495

Purpose

AiE2132m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Foff 2.0-ChR2-EYFP

Plasmid

#137163

Purpose

Intersectional viral expression of ChR2-EYFP in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-ChR2-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

H134R

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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AiP1047-pAAV-mscRE16-minBGpromoter-iCre-WPRE-hGHpA

Plasmid

#163479

Purpose

Direct-expressing iCre AAV Virus. Alias: AiP1047 - pAAV-AiE2016m-minBG-iCre-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

iCre

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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AiP14924 - pAAV-AiE0675m-minBG-iCre(297T)-BGHpA

Plasmid

#233571

Purpose

AiE0675m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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AAV CMV-driven AcrIIC3-scaffold (2xBsmBI sites)

Plasmid

#120301

Purpose

AAV vector for expression of AcrIIC3 (no miR binding sites, control vector)

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIC3

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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AiP1774 - pAAV-AiE2449m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214540

Purpose

AiE2449m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-DIO-GtACR2-P2A-Voltron2ST-W3SL

Plasmid

#217676

Purpose

AAV-mediated, Cre-dependent co-expression of GtACR2 and soma-targeted Voltron2 (ORCHID) for assessing inhibitory receptor driving force through voltage imaging with concurrent activation of GtACR2.

Depositor

Joseph Raimondo

Article

Selfe et al Nat Commun. 2024 Oct 16;15(1):8913. doi:

Has Service

AAV1

Insert

GtACR2-P2A-Voltron2ST

Use

AAV

Tags

Soma targeting sequence (Kv2.1) on Voltron2 gene …

Expression

Mammalian

Promoter

EF‐1α

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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p226-AAV-dlx-dio-mScarlet-T2A-SYPEGFP

Plasmid

#185696

Purpose

AAV vector expressing cre-dependent mem-mScarlet, T2A, Synaptophysin-EGFP driven by Dlx promoter

Depositor

Lynette Lim

Insert

mScarlet, T2A, Synaptophysin-EGFP

Use

AAV and Cre/Lox

Tags

mScarlet palmitoylation, Synaptophysin fused to E…

Expression

Mammalian

Promoter

mDlx

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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AiP995-pAAV-mscRE10-minBGpromoter-EGFP-WPRE-hGHpA

Plasmid

#163485

Purpose

Direct-expressing EGFP AAV Virus. Alias: AiP995 - pAAV-AiE2010m-minBG-EGFP-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

EGFP

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-SCP1-dSa VPR mini.-2X snRP-1 Neurog2

Plasmid

#99694

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9 and gRNA targeting Actc1 (gRNA: GGTATATAAGGGGTTTTAAG) (Actc1 Mouse, Synthetic)

Use

AAV and CRISPR

Tags

VPR mini

Mutation

dead Cas9

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6m-WPRE-pA

Plasmid

#51473

Purpose

Bicistronic vector expressing mRuby2 and GCaMP6m from a single open reading frame.

Depositor

Tobias Bonhoeffer, Mark Huebener, Tobias Rose

Article

Rose et al Science. 2016 Jun 10;352(6291):1319-22.

Insert

mRuby2-P2A-GCaMP6m

Use

AAV

Promoter

hSyn1

Available Since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

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AiP1894 - pAAV-AiE2582m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214573

Purpose

AiE2582m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Camk2a(0.4)-DIO-Opto-cytTrkB(E281A)-HA

Plasmid

#180588

Purpose

Opto-cytTrkB

Depositor

Won Do Heo

Article

Hong J et al J Mol Biol. 2020 Feb 14;432(4):815-827.

Insert

TrkB

Use

AAV

Mutation

PHR(E281A)

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pOTTC468 - pAAV EF1a DIO hChR2(H134R)-iRFP

Plasmid

#47633

Purpose

An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to iRFP) in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

hChR2 (H134R)

Use

AAV and Cre/Lox

Tags

iRFP

Expression

Mammalian

Mutation

H134R

Promoter

EF1a

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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