We narrowed to 159,481 results for: addgene

1. pCAG-HIF1 alpha

   Plasmid

   #21101

   Depositor

   Connie Cepko

   Article

   Chen et al Science. 2009 Jan 9. 323(5911):256-9.

   Insert

   HYPOXIA INDUCIBLE FACTOR 1ALPHA (HIF1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-AAVS1

   Plasmid

   #227272

   Purpose

   Expresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting AAVS1 (AAVS1 Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

   Plasmid

   #185919

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. ampl43

   Plasmid

   #161830

   Purpose

   pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

   Depositor

   Saeed Tavazoie

   Article

   Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIVM_02

   Plasmid

   #204500

   Purpose

   Expression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)

   Depositor

   Alessio Ciulli

   Article

   Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

   Insert

   amino acids 54-213 VHL (VHL Human)
   

   Use

   Tags

   6xHis, TEV, and Thrombin

   Expression

   Bacterial

   Mutation

   0

   Promoter

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSL1-E2C

   Plasmid

   #180421

   Purpose

   Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes an E2 Crimson promoter.

   Depositor

   Nancy Moran

   Article

   Li et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   E2 Crimson
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRRL MND GFP

   Plasmid

   #36247

   Depositor

   David Rawlings

   Article

   Astrakhan et al Blood. 2012 Mar 19.

   Insert

   GFP (eGFP )
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   MND

   Available since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX304 Luciferase-V5 blast

   Plasmid

   #98580

   Purpose

   Lentiviral vector for constitutive expression of Luciferase with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   Luciferase
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. USP25_pCSdest

   Plasmid

   #53817

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   USP25 (USP25 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

10. ChromosomesMembranesandCytoskeleton

    Plasmid

    #62450

    Purpose

    MXS_chaining vector with 4 cassettes

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Inserts

    CMV::H2B-TagBFPx3-bGHpA
    CMV::Lyn-Ceruleanx3-bGHpA
    CMV::mCherryx3-linker-betaActin-bGHpA
    CMV::Citrinex3-linker-alphaTubulin-bGHpA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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