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Plasmid#222204PurposeBacterial expression of mouse PTER-Y263F proteinDepositorInsertPTER (Pter Mouse)
UseTags6xHis and StrepExpressionBacterialMutationY263FPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29a-DDX53-Q249A
Plasmid#222216PurposeExpress DDX53 mutant Q249A protein in bacteriaDepositorInsertDDX53_Q249A (DDX53 Human)
UseTagsHis TagExpressionBacterialMutationK272APromoterT7Available sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29a-DDX53-K272A
Plasmid#222215PurposeExpress DDX53 mutant K272A protein in bacteriaDepositorInsertDDX53_K272A (DDX53 Human)
UseTagsHis TagExpressionBacterialMutationK272APromoterT7Available sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29a-DDX53-E377Q
Plasmid#222217PurposeExpress DDX53 mutant E377Q protein in bacteriaDepositorInsertDDX53_E377Q (DDX53 Human)
UseTagsHis TagExpressionBacterialMutationE377QPromoterT7Available sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-C675S
Plasmid#216756PurposeInvestigation of the potential interaction of the FeoB Cys675 residue with ferrous ironDepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationAmino acid C675 is substituted with S.PromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-C429S
Plasmid#216755PurposeInvestigation of the potential interaction of the FeoB Cys429 residue with ferrous ironDepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationAmino acid C429 is substituted with S.PromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-D123N
Plasmid#216754PurposeProduction of a FeoB that is unable to bind GTP for a negative control in GTPases assaysDepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationAmino acid D123 is substituted with N.PromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41SIII-ATF6(1-373)
Plasmid#171074PurposeExpresses of ATF6α(1-373) in bacteriaDepositorInsertATF6α (ATF6 Human)
UseTagsGST and HisExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Ideonella-bGSDM-WELQ
Plasmid#222098PurposeFor expression of a mutant bacterial gasdermin (bGSDM) encoded by a Ideonella species with a WELQut protease cleavage site for controlled activation.DepositorInsertIdeonella-bGSDM-WELQ ORF
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Vitiosangium-bGSDM-thrombin
Plasmid#222099PurposeFor expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.DepositorInsertVitiosangium-bGSDM-thrombin ORF
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only