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pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta1

Plasmid

#106114

Purpose

Lentiviral expression of PRKRA delta1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Lentiviral

Tags

DDK (FLAG) and myc

Expression

Mutation

AA 100-313

Promoter

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta23

Plasmid

#106117

Purpose

Lentiviral expression of PRKRA delta23

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Lentiviral

Tags

DDK (FLAG) and myc

Expression

Mutation

AA 1-127

Promoter

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS10_K138/139R

Plasmid

#127138

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS10 (RPS10 Human)

Use

Lentiviral

Tags

Expression

Mutation

K138R/K139R

Promoter

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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FH95pUp95GW (B4)

Plasmid

#74013

Purpose

lentiviral expression of shRNA resistant Psd95-alpha-EGFP fusion and Psd95 shRNA

Depositor

Robert Malenka, Weifeng Xu, Oliver Schluter

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

Psd95 shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta3

Plasmid

#106115

Purpose

Lentiviral expression of PRKRA delta3

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Lentiviral

Tags

DDK and myc

Expression

Mutation

Truncation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta12

Plasmid

#106116

Purpose

Lentiviral expression of PRKRA delta 12

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Lentiviral

Tags

Expression

Mutation

Truncation

Promoter

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_TARBP2

Plasmid

#106119

Purpose

Lentiviral expression of TARBP2

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

TARBP2 (TARBP2 Human)

Use

Lentiviral

Tags

DDK (FLAG) and Myc

Expression

Mutation

Promoter

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pGWT35S-VC155

Plasmid

#194051

Purpose

Transient expression of VN155 (mVenus β-strands 8 to 11, amino acids 155−239) in plant cell (Cytosol)

Depositor

Yutaka Kodama

Article

Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

Insert

VC155 (mVenus β-strands 8 to 11, amino acids 155−239)

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pGWT35S-VN154

Plasmid

#194050

Purpose

Transient expression of VN154 (mVenus β-strands 1 to 7, amino acids 1−154) in plant cell (Cytosol)

Depositor

Yutaka Kodama

Article

Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

Insert

VN154 (mVenus β-strands 1 to 7, amino acids 1−154)

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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FH95pUG+p97W (B8)

Plasmid

#74017

Purpose

lentiviral expression of Psd95 shRNA, and EGFP-SAP97beta fusion

Depositor

Robert Malenka, Weifeng Xu, Oliver Schluter

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

Psd95 shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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FH95pUp97aGW (C3)

Plasmid

#74019

Purpose

lentiviral expression of Psd95 shRNA and SAP97alpha-EGFP fusion

Depositor

Robert Malenka, Weifeng Xu, Oliver Schluter

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

Psd95 shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestMYC_RBPMS2

Plasmid

#65668

Purpose

MYC expression vector with RBPMS2

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS2 (RBPMS2 Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

introduced HindIII site to exchange FLAG-HA tag w…

Promoter

CMV, 2x Tet

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS_R38E

Plasmid

#65665

Purpose

FLAG-HA expression vector with RBPMS carrying mutation R38E

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

changed arginine 38 to glutamic acid

Promoter

CMV, 2x Tet

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS_R38A_E39A

Plasmid

#65664

Purpose

FLAG-HA expression vector with RBPMS carrying mutation R38A, E39A

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

changed arginine 38 to alanine, and glutamic acid…

Promoter

CMV, 2x Tet

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pLIB-RSP5

Plasmid

#176694

Purpose

Expression of Chlamydomonas reinhardtii RSP5 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP5 (RSP5 Chlamydomonas reinhardtii)

Use

Tags

Expression

Insect

Mutation

None

Promoter

polyhedrin

Available since

Availability

Academic Institutions and Nonprofits only

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pEC3006 (pSpy1C-PZn_ffluc)

Plasmid

#218515

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenes

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Tags

Expression

Bacterial

Mutation

deletion of Plac_mrfp cassette

Promoter

PZn (PczcD)

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-HLA-A2_GFP

Plasmid

#85161

Purpose

HLA.A2_GFP fusion with 2 glycines in between

Depositor

Sébastien Walchli, Tone Fredsvik Gregers

Article

Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

Insert

HLA.A2 (HLA-A Human)

Use

Gateway entry vector

Tags

EGFP

Expression

Mutation

Promoter

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3_Keima

Plasmid

#127140

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3_Keima (RPS3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRE145

Plasmid

#118497

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE132

Plasmid

#118486

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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