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pFBOH-MHL.mEGFP-CBX8

Plasmid

#224710

Purpose

Expresses N-terminal hexahistidine-tagged human CBX8 in insect cells, with a monomeric variant EGFP tag.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 (CBX8 Human)

Use

Tags

mEGFP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Oct. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFB1.HMBP.PrS.CBX8-KR21A

Plasmid

#224709

Purpose

Expresses a human CBX8 mutant (KR21A) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 KR21A mt (CBX8 Human)

Use

Tags

HMBP

Expression

Insect

Mutation

Promoter

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.CBX8

Plasmid

#224707

Purpose

Expresses human CBX8 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 (CBX8 Human)

Use

Tags

HMBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_A8_His

Plasmid

#201718

Purpose

Expression of nanobody

Depositor

Ray Owens

Article

Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

Insert

VHH_A8 (S lama glama (llama))

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Promoter

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_A10-5_His

Plasmid

#201720

Purpose

Expression of nanobody

Depositor

Ray Owens

Article

Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

Insert

VHH_A10-5 (S lama glama (llama))

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Promoter

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_B5-5_His

Plasmid

#201721

Purpose

Expression of nanobody

Depositor

Ray Owens

Article

Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

Insert

VHH B5-5 (S lama glama (llama))

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Promoter

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.RBBP4

Plasmid

#125164

Purpose

Expresses human RBBP4 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Histone-binding protein RBBP4 (RBBP4 Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.EZH2

Plasmid

#125161

Purpose

Expresses human EZH2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

EZH2 (EZH2 Human)

Use

Baculovirus expression vector

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.AEBP2

Plasmid

#125165

Purpose

Expresses human AEBP2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Zinc finger protein AEBP2 (AEBP2 Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.EED

Plasmid

#125163

Purpose

Expresses human EED in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Polycomb protein EED (EED Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.SUZ12

Plasmid

#125162

Purpose

Expresses human SUZ12 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Polycomb protein SUZ12 (SUZ12 Human)

Use

Baculovirus expression vector

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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WT (DVD)

Plasmid

#184316

Purpose

To study dynamics of G-actin using NMR spectroscopy.

Depositor

Michael Rosen

Article

Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

Insert

Act5C (Act5C Fly)

Use

Tags

Hexahistidine followed by B-thymosin

Expression

Yeast

Mutation

Promoter

AOX1

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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RP1B FUS 1-163 S12xQ

Plasmid

#127193

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Article

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 S12xQ (FUS Human)

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

S12xQ: S30Q, S44Q, S48Q, S53Q, S70Q, S84Q, S89Q, …

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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SKM2 X057_pECIA2

Plasmid

#115114

Purpose

Bait vector SKM2 X057_pECIA2 should be used with prey vector SKM2 X057_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G56040 (AT5G56040 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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BAM1 X060_pECIA2

Plasmid

#115117

Purpose

Bait vector BAM1 X060_pECIA2 should be used with prey vector BAM1 X060_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G65700 (BAM1 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PXC3 X011_pECIA2

Plasmid

#115118

Purpose

Bait vector PXC3 X011_pECIA2 should be used with prey vector PXC3 X011_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G41820 (AT2G41820 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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EFR D5_pECIA2

Plasmid

#115119

Purpose

Bait vector EFR D5_pECIA2 should be used with prey vector EFR D5_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G20480 (EFR Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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FLS2 FLS2_pECIA2

Plasmid

#115120

Purpose

Bait vector FLS2 FLS2_pECIA2 should be used with prey vector FLS2 FLS2_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G46330 (FLS2 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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ERECTA C6_pECIA2

Plasmid

#115127

Purpose

Bait vector ERECTA C6_pECIA2 should be used with prey vector ERECTA C6_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G26330 (ER Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CEPR2 X048_pECIA2

Plasmid

#115099

Purpose

Bait vector CEPR2 X048_pECIA2 should be used with prey vector CEPR2 X048_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G72180 (AT1G72180 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge