We narrowed to 527 results for: plasmid cloning by pcr
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Plasmid#200764PurposeExpresses Superfolder GFP-tag fused single change variable fragment antibody of GCN4 (Suntag) with the tissue-specific promoter col-19 in C. elegans.DepositorInsertscFv-sfGFP
ExpressionWormPromotercol-19Available SinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCgsgRNA_crtYf
Plasmid#153338PurposeDouble-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA targeting crtYf; SpcrDepositorInsertcrRNA targeting crtYf
UseCRISPRExpressionBacterialAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDD428
Plasmid#202081PurposeCas9/sgRNA plasmid targeting the C-terminus of Myh9DepositorAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJEC726
Plasmid#190814PurposeCRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.DepositorInsertdCas9
UseCRISPR and Synthetic BiologyMutationS. pyogenes dCas9 codon optimized for Streptomyce…PromoterSP30Available SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
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Nanog-2A-mCherry
Plasmid#59995PurposeInserts mCherry immediately downstream of the Nanog coding regionDepositorAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Nanog-2A-GFP
Plasmid#59994PurposeInserts GFP immediately downstream of the Nanog coding regionDepositorAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
eGFPbait-E2A-KalTA4-pA donor vector
Plasmid#61069Purposefor CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051)DepositorInsertseGFPbait
KalTA4
UseZebrafish expressionTagsE2AAvailable SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
ptia1l-hsp-GFP
Plasmid#177837PurposeDonor plasmid for knock-in of hsp70-EGFP using CRISPR-Cas9DepositorInserttia1l-sgRNA target site-hsp70-EGFP
UseCRISPRTagsEGFPPromoterhsp70Available SinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-NE2m
Plasmid#208686PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m in neuronsDepositorInsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2m
UseAAVPromoterhSynAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-NE2h
Plasmid#208687PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h in neuronsDepositorHas ServiceAAV5InsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2h
UseAAVPromoterhSynAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_OT1.0
Plasmid#185386PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in neuronsDepositorHas ServiceAAV1 and AAV9InsertGPCR activation based oxytocin sensor GRAB_OT1.0
UseAAVPromoterhSynAvailable SinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_OT1.0
Plasmid#185387PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent mannerDepositorInsertGPCR activation based oxytocin sensor GRAB_OT1.0
UseAAVPromoterEFSAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_OTmut
Plasmid#185388PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neuronsDepositorHas ServiceAAV1 and AAV9InsertGPCR activation based oxytocin control sensor GRAB_OTmut
UseAAVPromoterhSynAvailable SinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_OTmut
Plasmid#185389PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent mannerDepositorInsertGPCR activation based oxytocin control sensor GRAB_OTmut
UseAAVPromoterEFSAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pToxAmp21R
Plasmid#218287PurposeA complete plasmid for ToxAmp (Toxin-antitoxin-driven gene amplification) for the expression of AeBlueDepositorInsertHO(-955, -789)-LoxP>pKlLEU2>KlLEU2>PCRT1>RelB>tPDC1-pTDH3>AeBlue>tSYNth7-ARS712-HO(-731, -264)
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-CMV-GRAB_LoX3-mut
Plasmid#234651PurposeThis plasmid encodes a mutated version of the genetically encoded chemokine biosensor LoX3-1.0, which is designed to be non-responsive to chemokine binding.DepositorInsertGPCR activation-based mutant chemokine CXCL9-11 sensor GRAB_LoX3-mut
ExpressionMammalianPromoterCMVAvailable SinceAug. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
PuroR-P2A-DD-TCOF1-donor
Plasmid#60747PurposeDonor plasmid for HR-mediated recombination to insert puromycin resistance and append a destabilization domain to the N-terminus of TCOF1DepositorInsertTCOF1 (TCOF1 Human)
UseTargeting donorAvailable SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
BlastR-P2A-DD-TCOF1-donor
Plasmid#60748PurposeDonor plasmid for HR-mediated recombination to insert blasticidin resistance and append a destabilization domain to the N-terminus of TCOF1DepositorInsertTCOF1 (TCOF1 Human)
UseTargeting donorAvailable SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_NE1h
Plasmid#123309PurposeExpresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h in neuronsDepositorHas ServiceAAV Retrograde and AAV9InsertGPCR activation based NE sensor GRAB_NE1h
UseAAVPromoterhSynAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only