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AI-CN442

Plasmid

#62591

Purpose

TALEN array receiving vector with half-site HD RVD

Depositor

Joshua Grimley

Publication

Martinez et al Nucleic Acids Res. 2015 Mar 11. pii: gkv

Type

Empty backbone

Use

TALEN; T7

Tags

3× FLAG and FOKI WT

Expression

Mammalian

Promoter

CMV

Available since

March 24, 2015

Availability

Academic Institutions and Nonprofits only

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AI-CN441

Plasmid

#62590

Purpose

TALEN array receiving vector with half-site NI RVD

Depositor

Joshua Grimley

Publication

Martinez et al Nucleic Acids Res. 2015 Mar 11. pii: gkv

Type

Empty backbone

Use

TALEN; T7

Tags

3× FLAG and FOKI WT

Expression

Mammalian

Promoter

CMV

Available since

March 24, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MAVS-M1A500(KaganF60)

Plasmid

#52003

Purpose

Expresses the human MAVS CDS containing a point mutation M1A and a C-terminal deletion removing the transmembrane domain

Depositor

Jonathan Kagan

Publication

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A-500

Expression

Mammalian

Mutation

changed Met 1 to Ala and deleted the C-terminal t…

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
1132 pME-Lin28

Plasmid

#49009

Purpose

full length coding sequence for zebrafish lin28 gene in gateway middle entry vector

Depositor

Nathan Lawson

Publication

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

lin-28 homolog A (lin28ab Zebrafish)

Use

Gateway middle entry

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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666 pME-Lnx-1

Plasmid

#49006

Purpose

full length coding sequence for zebrafish lnx1 gene in gateway middle entry vector

Depositor

Nathan Lawson

Publication

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ligand of numb-protein X 2b (lnx2b Zebrafish)

Use

Gateway middle entry

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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UAS-Myc-dASUN

Plasmid

#47036

Purpose

Drosophila expression of tagged dASUN

Depositor

Laura Lee

Publication

Anderson et al Mol Biol Cell. 2009 Jun;20(11):2709-21.

Insert

Asunder (asun Fly)

Tags

Myc

Expression

Insect

Available since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
TV3-mCHY-dASUN

Plasmid

#47034

Purpose

Drosophila testes specific expression of fluorescent dASUN

Depositor

Laura Lee

Publication

Anderson et al Mol Biol Cell. 2009 Jun;20(11):2709-21.

Insert

Asunder (asun Fly)

Tags

mCHY

Expression

Insect

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only
TV3-eGFP-dASUN

Plasmid

#47033

Purpose

Drosophila testes specific expression of fluorescent dASUN

Depositor

Laura Lee

Publication

Anderson et al Mol Biol Cell. 2009 Jun;20(11):2709-21.

Insert

Asunder (asun Fly)

Tags

eGFP

Expression

Insect

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only
pCDF1-ADAMTS18 (P1035S)

Plasmid

#29558

Depositor

Yardena Samuels

Publication

Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

Insert

ADAMTS18 (ADAMTS18 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed proline 1035 to serine

Available since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only
pCDF1-ADAMTS18 (Q1002X)

Plasmid

#29557

Depositor

Yardena Samuels

Publication

Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

Insert

ADAMTS18 (ADAMTS18 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed glutamine 1002 to stop codon

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pCDF1-ADAMTS18 (Q904X)

Plasmid

#29556

Depositor

Yardena Samuels

Publication

Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

Insert

ADAMTS18 (ADAMTS18 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed glutamine 904 to stop codon

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pCDF1-ADAMTS18 (C638S)

Plasmid

#29555

Depositor

Yardena Samuels

Publication

Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

Insert

ADAMTS18 (ADAMTS18 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed cysteine 638 to serine

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pCDF1-ADAMTS18 (G312E)

Plasmid

#29553

Depositor

Yardena Samuels

Publication

Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

Insert

ADAMTS18 (ADAMTS18 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed glycine 312 to glutamic acid

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
OSR1-pCS2+MT

Plasmid

#30491

Depositor

Gerhart Ryffel

Publication

Drews et al BMC Dev Biol. 2011 Jan 31;11:5. doi: 10.

Insert

OSR1 (OSR1 Human)

Tags

6xMyc

Expression

Mammalian

Available since

Aug. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pFLAG rat Antizyme 1

Plasmid

#11183

Depositor

Bruce Zetter

Publication

Newman et al J Biol Chem. 2004 Oct 1. 279(40):41504-1

Insert

AZ1 (Oaz1 Rat)

Tags

Flag

Expression

Mammalian

Available since

Dec. 17, 2005

Availability

Academic Institutions and Nonprofits only

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CLTA-GFP HDRT Source (pTR 153)

Plasmid

#112016

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with GFP

Depositor

Alexander Marson

Publication

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CLTA-GFP HDRT (CLTA Human)

Use

CRISPR and Synthetic Biology

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Flag-SPRTN-E112A

Plasmid

#110215

Purpose

Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.

Depositor

Ivana Marinovic-Terzic, Kristijan Ramadan

Publication

Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

Insert

SPRTN (SPRTN Human)

Tags

Flag

Expression

Mammalian

Mutation

E112A, P296L (see depositor comments below)

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNH-NanoLuc

Plasmid

#173075

Purpose

Expression vector for E. coli producing target protein with N-terminal Nanoluciferase fusion

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEX6P1-UBE2S-UBD

Plasmid

#66713

Purpose

Expresses the UBE2S-UBD fusion protein in E. coli

Depositor

David Komander

Publication

Bremm et al Nat Struct Mol Biol. 2010 Aug;17(8):939-

Inserts

e2-epf (UBE2S )
isot (USP5 )

Tags

GST-3C

Expression

Bacterial

Promoter

T7

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
CD4-C-GFP HDRT Source (pTR 152)

Plasmid

#112018

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with GFP

Depositor

Alexander Marson

Publication

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-GFP HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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