We narrowed to 19,551 results for: ACE

1. pE-FLP

   Plasmid

   #45978

   Purpose

   Expresses yeast Flp driven by the constitutive promoter pE from phage P2

   Depositor

   Keith Shearwin, Drew Endy

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Insert

   flp
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   flp driven by the constitutive promoter pE from p…

   Promoter

   pE (from phage P2)

   Available since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag

   Plasmid

   #209651

   Purpose

   Expression of iATPSnFR2, high affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.S29W.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   S29W.A95K

   Promoter

   CAG

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19_CRISPR_DpmrA

   Plasmid

   #160903

   Purpose

   CRISPR_Cas9, lambda red recombineering

   Depositor

   Anne-Catrin Uhlemann

   Article

   McConville et al Cell Rep. 2020 Oct 27;33(4):108313. doi:

   Inserts

   Cas9
   Lambda Red Recombineering Genes
   Zeocin Resistance Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   araBAD

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. LXRalpha_pcDNA3.1

   Plasmid

   #177621

   Purpose

   Full-length human LXR alpha cDNA sequence in pcDNA3.1+

   Depositor

   Thomas Burris

   Article

   Griffett et al ACS Chem Biol. 2022 Apr 13. doi: 10.1021

   Insert

   LXR alpha (NR1H3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-Cys-ELP(40nm)-LPETGG

   Plasmid

   #90475

   Purpose

   Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

   Depositor

   Hermann Gaub

   Article

   Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

   Insert

   Cys-ELP(40nm)-LPETGG
   

   Use

   Tags

   Cysteine and Sortase-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCGN-HCF-1 fl

   Plasmid

   #53309

   Purpose

   plasmid contains human HCFC1 full length gene with HA and c-myc tags

   Depositor

   Winship Herr

   Article

   Wilson et al Cell. 1993 Jul 16;74(1):115-25.

   Insert

   HCFC1 (HCFC1 Human)
   

   Use

   Tags

   HA and c-myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-GGG-ELP(40nm)-Cys

   Plasmid

   #91571

   Purpose

   Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

   Depositor

   Hermann Gaub

   Article

   Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

   Insert

   GGG-ELP(40nm)-Cys
   

   Use

   Tags

   Cysteine and Sortase-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pre_II_CS3_7653_v2

   Plasmid

   #126855

   Purpose

   7653 b scaffold from compatibility group 3, made by method II, containing 1 Zn-dependent self-cleaving DNAzyme cassette for linearisation into 7560 b scaffold

   Depositor

   Hendrik Dietz

   Article

   Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

   Insert

   II_CS3_7653_v2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. VPR-ps-dSpCas9

   Plasmid

   #102855

   Purpose

   A single-chain light-controllable dSpCas9 with pdDronpa1.2 domains

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   dSpCas9, pdDronpa1.2
   

   Use

   CRISPR and Synthetic Biology

   Tags

   NLS and VP64-p64-Rta transcription activator

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Golgi-TurboID

    Plasmid

    #200959

    Purpose

    Transiently expresses golgi localized TurboID with HA tags

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    Golgi-TurboID
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-FLEX-SaCas9-U6-sgSlc18a2

    Plasmid

    #124849

    Purpose

    Mutagenesis of Slc18a2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc18a2 (Slc18a2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-FLEXfrt-SaCas9-U6-sgSlc18a2

    Plasmid

    #124862

    Purpose

    Mutagenesis of Slc18a2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc18a2 (Slc18a2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSCD-GSDMA-N-tev-C

    Plasmid

    #226231

    Purpose

    Express TEVP-cleavable GSDMA

    Depositor

    Michael Elowitz

    Article

    Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

    Insert

    Gasdermin A (Gsdma3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AMPK-SPARK

    Plasmid

    #226255

    Purpose

    phases-of-separation based activity reporter of AMPK

    Depositor

    Roland Malli

    Article

    Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

    Insert

    AMPK-SPARK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pTU1-A-SP44-sfGFP

    Plasmid

    #163757

    Purpose

    Expresses Streptomyces codon-optimised sfGFP

    Depositor

    Paul Freemont, Simon Moore

    Article

    Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    SP44

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV sgRNA Expression Plasmid

    Plasmid

    #174540

    Purpose

    Contains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker.

    Depositor

    Ormond MacDougald

    Article

    Romanelli et al J Biol Chem. 2021 Nov 11:101402. doi: 10

    Type

    Empty backbone

    Use

    AAV

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209653

    Purpose

    Expression of iATPSnFR2, low affinity

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    A95A.A119L

    Promoter

    CAG

    Available since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. eBFP2 (eBFP2)_CD

    Plasmid

    #66034

    Purpose

    MoClo Basic Part: CDS - Fluorescent protein. Blue. Derived from Addgene#14891. [C:eBFP2:D]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    Fluorescent reporter - BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-PITCh-PXN

    Plasmid

    #227315

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PXN for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of PXN (PXN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21b-Caspase-3

    Plasmid

    #90087

    Purpose

    Expresses wild type caspase 3 in bacterial cells

    Depositor

    Clay Clark

    Article

    Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Wild type

    Promoter

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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