We narrowed to 6,558 results for: sas;

1. pMS21

   Plasmid

   #229476

   Purpose

   Expresses RssB with E135A, E136A, & E137A mutation with His-tag

   Depositor

   Alexandra Deaconescu

   Article

   Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

   Insert

   RssB (E135A, E136A, E137A)
   

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   E135A, E136A, E137A

   Promoter

   T7

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVF18

   Plasmid

   #229456

   Purpose

   Expresses IraD (aa17-130) with His-tag

   Depositor

   Alexandra Deaconescu

   Article

   Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

   Insert

   IraD (aa 17-130 only)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   aa 17-130 only

   Promoter

   T7

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHIT239

   Plasmid

   #204522

   Purpose

   In vitro transcription of Mos1 transposase mRNA with SL1 and poly(A) used for microinjection into nematode

   Depositor

   Yuji Kohara

   Article

   Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

   Insert

   Mos1_transposase::glh-2_3'UTR (glh-2 Nematode)
   

   Use

   Other

   Promoter

   T7 (minimum seq.)

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUCSh2-mRFP

   Plasmid

   #170982

   Purpose

   Encodes sgRNA scaffold with mRFP in place of target and Kanamycin resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

   Depositor

   Christopher Reisch

   Article

   Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

   Insert

   mRFP
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Mdn1-FL

   Plasmid

   #171602

   Purpose

   Expresses full length S. pombe Mdn1 in insect cells.

   Depositor

   Tarun Kapoor

   Article

   Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

   Insert

   Mdn1
   

   Tags

   His6 tag

   Expression

   Insect

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKI-cjOCT4-Cyan-pac

   Plasmid

   #160871

   Purpose

   Marmoset Targeting

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Stem Cell Res. 2021 Mar 24;53:102308. do

   Insert

   mCerulean3
   

   Use

   Marmoset targeting

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAS1_4xh7SKPylT_EF1_BsmBI_(C1A)-Intein_DD

   Plasmid

   #162811

   Purpose

   Control STELLA destination plasmid for C-terminal labelled proteins. Contains restriction sites for golden gate cloning.

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   BsmBI_(C1A)-Intein_DD
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   Intein(Cys1,Ala)

   Promoter

   EF1alpha

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAS1_4x7SKPylT_EF1_Ub-*CoV2_M

   Plasmid

   #162815

   Purpose

   STELLA plasmid for amber suppression of N-terminally labelled CoV2_M

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   Ub-(N-TAG)CoV2_M
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   CoV2_M(Met1,Amber)

   Promoter

   EF1alpha

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS1_4x7SKPylT_EF1_Ub-*CoV2_9b

   Plasmid

   #162816

   Purpose

   STELLA plasmid for amber suppression of N-terminally labelled CoV2_9b

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   Ub-(N-TAG)CoV2_9b
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   CoV2_9b(Met1,Amber)

   Promoter

   EF1alpha

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF6

    Plasmid

    #162817

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF6

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF6
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    CoV2_ORF6(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF7b

    Plasmid

    #162818

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF7b

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF7b
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    CoV2_ORF7b(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF10

    Plasmid

    #162819

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF10

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF10
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    CoV2_ORF10(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF9c/14

    Plasmid

    #162820

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF9c/14

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF9c/14
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    CoV2_ORF9c/14(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b_alt

    Plasmid

    #162821

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b_alt

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF3b_alt
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    CoV2_ORF3b_alt(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b(CoV1h)

    Plasmid

    #162822

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b(CoV1h)

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF3b(CoV1h)
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    CoV2_ORF3b(CoV1h)(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD

    Plasmid

    #162805

    Purpose

    STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-BsmBI-HA_IRES_Blasticidin
    

    Use

    Piggybac

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC_h7SK M15 (CUA)

    Plasmid

    #154784

    Purpose

    plasmid with single copy of Mma PylT M15 mutant (CUA anticodon)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    Mma PylT
    

    Expression

    Mammalian

    Mutation

    G10A, G14U, G16U, C20U, C52A

    Promoter

    h7SK

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR2-L3-SfoI-Venus-PBL-R1

    Plasmid

    #141019

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    mVenus
    

    Expression

    Mammalian

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR-L3-Csy4-Cas9-EGFP-L2

    Plasmid

    #141045

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Csy4-2A-Cas9-2A-EGFP
    

    Expression

    Mammalian

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-L3-eSpCas9(1.1)-2A-EGFP-L2

    Plasmid

    #141051

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    eSpCas9(1.1)-2A-EGFP
    

    Expression

    Mammalian

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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