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Negative feedback (NF) plasmid

Plasmid

#169747

Purpose

Standalone NF circuit plasmid that has the same implementation of the NF subcircuit in Equalizers.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

tetR-P2A-eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV-tetO2

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA E59Q, TadA* V106W)

Plasmid

#126496

Purpose

Expresses ABEmax(TadA E59Q, TadA*V106W) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59Q TadA* V106W)

Expression

Mammalian

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA E59, TadA* V106W)

Plasmid

#126497

Purpose

Expresses ABEmax(TadA E59, TadA*V106W) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59 TadA* V106W)

Expression

Mammalian

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pHIE061 ZF43x6-C mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138742

Purpose

Integration Vector, assembled with pHIE045 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJM457 EF1alpha EBFP2-P2A-BlastR (TUPV8)

Plasmid

#139237

Purpose

mMoClo TUPV8, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pJM454 EF1alpha EBFP2-P2A-BlastR (TUPV5)

Plasmid

#139234

Purpose

mMoClo TUPV5, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pJM453 EF1alpha EBFP2-P2A-BlastR (TUPV4)

Plasmid

#139233

Purpose

mMoClo TUPV4, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJM452 EF1alpha EBFP2-P2A-BlastR (TUPV3)

Plasmid

#139232

Purpose

mMoClo TUPV3, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJM451 EF1alpha EBFP2-P2A-BlastR (TUPV2)

Plasmid

#139231

Purpose

mMoClo TUPV2, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pPD270 ZF43x6-Compact(overlapping ZF37)_EYFP

Plasmid

#138934

Purpose

EYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact(overlapping ZF37)

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x6-Compact(overlapping ZF37)

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pPD150 ZF43x12-Spaced-Far_EYFP

Plasmid

#138895

Purpose

EYFP reporter vector for the ZF43x12-Spaced-Far promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Spaced-Far

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x12-Spaced-Far

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pPD147 ZF43x8-Spaced-Far_EYFP

Plasmid

#138893

Purpose

EYFP reporter vector for the ZF43x8-Spaced-Far promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x8-Spaced-Far

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x8-Spaced-Far

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pPD145 ZF43x4-Spaced-Far_EYFP

Plasmid

#138891

Purpose

EYFP reporter vector for the ZF43x4-Spaced-Far promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x4-Spaced-Far

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x4-Spaced-Far

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pPD144 ZF43x2-Spaced-Far_EYFP

Plasmid

#138890

Purpose

EYFP reporter vector for the ZF43x2-Spaced-Far promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x2-Spaced-Far

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x2-Spaced-Far

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pPD140 ZF43x12-Spaced-Mid_EYFP

Plasmid

#138889

Purpose

EYFP reporter vector for the ZF43x12-Spaced-Mid promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Spaced-Mid

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x12-Spaced-Mid

Available Since

Jan. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pPD137 ZF43x8-Spaced-Mid_EYFP

Plasmid

#138887

Purpose

EYFP reporter vector for the ZF43x8-Spaced-Mid promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x8-Spaced-Mid

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x8-Spaced-Mid

Available Since

Jan. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pPD135 ZF43x4-Spaced-Mid_EYFP

Plasmid

#138885

Purpose

EYFP reporter vector for the ZF43x4-Spaced-Mid promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x4-Spaced-Mid

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x4-Spaced-Mid

Available Since

Jan. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pPD134 ZF43x2-Spaced-Mid_EYFP

Plasmid

#138884

Purpose

EYFP reporter vector for the ZF43x2-Spaced-Mid promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x2-Spaced-Mid

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x2-Spaced-Mid

Available Since

Jan. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pCbh-SpyCas9-2A-puro-pA_U6-BbsI

Plasmid

#149347

Purpose

Mammalian expression vector for SpyCas9 and puromycin resistance

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

SpyCas9

Use

CRISPR

Tags

Flag tag

Expression

Mammalian

Promoter

Cbh

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pPD882 FKBP-ZF43 (NES)

Plasmid

#138854

Purpose

Constitutive expression of FKBP-ZF43 (NES) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

FKBP-ZF43 (NES)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPD681 FKBP-ZF43 (no NLS)

Plasmid

#138848

Purpose

Constitutive expression of FKBP-ZF43 (no NLS) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

FKBP-ZF43 (no NLS)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pPD341 FKBP-ZF43 (NLS)

Plasmid

#138844

Purpose

Constitutive expression of FKBP-ZF43 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

FKBP-ZF43

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pPD173 ZF43(AAAA)i

Plasmid

#138833

Purpose

Constitutive expression of ZF43(AAAA)i under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43(AAAA)i

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R6A, R15A, R43A, R71A

Promoter

CMV

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pPD172 ZF43(AAAR)i

Plasmid

#138832

Purpose

Constitutive expression of ZF43(AAAR)i under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43(AAAR)i

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R6A, R15A, R43A

Promoter

CMV

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pPD171 ZF43(AARR)i

Plasmid

#138831

Purpose

Constitutive expression of ZF43(AARR)i under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43(AARR)i

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R6A, R15A

Promoter

CMV

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pPD169 ZF43(RAAA)i

Plasmid

#138829

Purpose

Constitutive expression of ZF43(RAAA)i under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43(RAAA)i

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R15A, R43A, R71A

Promoter

CMV

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pPD168 ZF43(RAAR)i

Plasmid

#138828

Purpose

Constitutive expression of ZF43(RAAR)i under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43(RAAR)i

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R15A, R43A

Promoter

CMV

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pHIE065 ZF43x12-S mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138746

Purpose

Integration Vector, assembled with pHIE049 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Spaced

Use

Synthetic Biology

Expression

Mammalian

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pPD389 VPR-ZF43(AAAA)

Plasmid

#138826

Purpose

Constitutive expression of VPR-ZF43(AAAA) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-ZF43(AAAA)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R6A, R15A, R43A, R71A

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pPD387 VPR-ZF43(AAAR)

Plasmid

#138825

Purpose

Constitutive expression of VPR-ZF43(AAAR) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-ZF43(AAAR)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R6A, R15A, R43A

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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