We narrowed to 6,933 results for: 11

1. pLenti-Puro-sgBRSK2

   Plasmid

   #138687

   Purpose

   Expresses a human BRSK2-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgBRSK2 human (BRSK2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-Blast-sgBRSK1

   Plasmid

   #138705

   Purpose

   Expresses a human BRSK1-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgBRSK1 human (BRSK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-Puro-sgBRSK1

   Plasmid

   #138686

   Purpose

   Expresses a human BRSK1-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgBRSK1 human (BRSK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-Neo-sgCRTC2-2

   Plasmid

   #138703

   Purpose

   Expresses a human CRTC2-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgCRTC2 human (CRTC2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCOG40-HRS (257-276) Hs

   Plasmid

   #83236

   Purpose

   pGST-HRS (257-276) Hs, Expresses the GST-HRS in E.coli

   Depositor

   Gali Prag

   Article

   Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

   Insert

   HRS (257-276) Hs (HGS Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   258-277

   Promoter

   Available since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMAL-PIAL2M-sim1

   Plasmid

   #67908

   Purpose

   Inducible expression of a mutant of PIAL2M where the SIM interacting motif 1 (VFDL 425-428) was substituted with alanines.

   Depositor

   Andreas Bachmair

   Article

   Tomanov et al Plant Cell. 2014 Nov;26(11):4547-60. doi

   Insert

   PIAL2 (AT5G41580 Mustard Weed)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   E281-A496; VEDL 425-428 AAA

   Promoter

   P-lac

   Available since

   Aug. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pMiR-CIP2A-stop-mut

   Plasmid

   #53713

   Purpose

   Luciferase reporter assay for CIP2A with mutated firefly stop codon

   Depositor

   Edward Chan

   Article

   Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

   Insert

   CIP2A (CIP2A Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Mutation on the stop codon of firefly luciferase …

   Promoter

   Available since

   July 2, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion (NT96)

   Plasmid

   #50914

   Purpose

   Expresses human NKCC1 with truncation of N-terminus and mutations at P676C I730C. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells

   Depositor

   Biff Forbush

   Article

   Monette et al J Biol Chem. 2014 Jan 22.

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   P676C I730C N-term deletion of aa13-222 in hNKCC1…

   Promoter

   CMV

   Available since

   Feb. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 Flag YFP hNKCC1 C723S C724V A735C (NT549)

   Plasmid

   #50910

   Purpose

   Expresses human NKCC1 C723S C724V A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Monette et al J Biol Chem. 2014 Jan 22.

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   C723S C724V A735C in hNKCC1 in NT17

   Promoter

   CMV

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1 Flag YFP hNKCC1 A675C C723S C724V A735C (NT847)

    Plasmid

    #50865

    Purpose

    Expresses human NKCC1 A675C C723S C724V A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    A675C C723S C724V A735C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1 Flag YFP hNKCC1 C723S C724V V729C (NT543)

    Plasmid

    #50907

    Purpose

    Expresses human NKCC1 C723S C724V V729C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    C723S C724V V729C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1 Flag YFP hNKCC1 C723S C724V W733C (NT547)

    Plasmid

    #50909

    Purpose

    Expresses human NKCC1 C723S C724V W733C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    C723S C724V W733C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1 Flag YFP hNKCC1 C723S C724V F728C (NT542)

    Plasmid

    #50906

    Purpose

    Expresses human NKCC1 C723S C724V F728C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    C723S C724V F728C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1 Flag YFP hNKCC1 C723S C724V M727C (NT540)

    Plasmid

    #50905

    Purpose

    Expresses human NKCC1 C723S C724V M727C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    C723S C724V M727C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NUDT1

    Plasmid

    #74660

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    NUDT1 (NUDT1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pBabepuro-IL11

    Plasmid

    #189609

    Purpose

    Retroviral expression vector for IL11

    Depositor

    Thierry Tchenio

    Insert

    IL11 (IL11 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    SV40 early promoter

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK614

    Plasmid

    #72387

    Purpose

    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylamine-glycine ligase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    contains 4 silent mutations in codons 9-11 and 42…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK621

    Plasmid

    #72388

    Purpose

    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylamine-glycine ligase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    contains 3 silent mutations in codons 9-11 to fac…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK592

    Plasmid

    #72386

    Purpose

    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurD)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylamine-glycine ligase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    contains 3 silent mutations in codons 9-11 to fac…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV.hSynapsin.SF-iGluSnFR.A184S

    Plasmid

    #106174

    Purpose

    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV1 and AAV9

    Insert

    SF-iGluSnFR.A184S
    

    Use

    AAV

    Tags

    Expression

    Mutation

    GltI: A184S

    Promoter

    hSynapsin

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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