We narrowed to 16,038 results for: GRN

1. SunTag-FWAgRNA-4-14aa-TET1cd

   Plasmid

   #106436

   Purpose

   SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter

   Depositor

   Steven Jacobsen

   Article

   Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

   Insert

   gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN414aa_OCS (TET1 Synthetic, Human, Mustard Weed)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-PpU6P-sgRNA-R4R3

   Plasmid

   #113739

   Purpose

   Gateway entry vector containing attR4 and attR3 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   PpU6P::sgRNA
   

   Use

   CRISPR; Gateway entry vector

   Promoter

   P. patens U6 promoter

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR-PpU6P-sgRNA-L5L4

   Plasmid

   #113741

   Purpose

   Gateway entry vector containing attL5 and attL4 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   PpU6P::sgRNA
   

   Use

   CRISPR; Gateway entry vector

   Promoter

   P. patens U6 promoter

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-PpU6P-sgRNA-L1R5

   Plasmid

   #113737

   Purpose

   Gateway entry vector containing attL1 and attR5 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   PpU6P::sgRNA
   

   Use

   CRISPR; Gateway entry vector

   Promoter

   P. patens U6 promoter

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZ147-BvCas12b-sgRNA-scaffold

   Plasmid

   #122447

   Purpose

   Expresses sgRNA for BvCas12b in mammalian cells

   Depositor

   Feng Zhang

   Article

   Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

   Insert

   U6-BvCas12b-sgRNA-scaffold, CMV-mCherry
   

   Expression

   Mammalian

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLH-sgRNA1-PP7-boxB

   Plasmid

   #75393

   Purpose

   sgRNA1-PP7-boxB

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   sgRNA1-2XPP7-boxB
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLH-sgRNA1-MS2-PP7

   Plasmid

   #75392

   Purpose

   sgRNA1-MS2-PP7

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   sgRNA1-2XMS2-PP7
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Expression scaffold for dCas9+sgRNA

   Plasmid

   #171799

   Purpose

   PICASSO: Expression scaffold for second subcloning step to properly co-express paired dCas9-fusion+sgRNA

   Depositor

   Stephen Elledge

   Article

   Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

   Insert

   PICASSO: expression scaffold for proper dCas9-fusion + sgRNA expression
   

   Use

   CRISPR

   Available Since

   Aug. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV2-U6gRNA5(Pou5f1-g2)-PGKpuroBFP-W

   Plasmid

   #105036

   Purpose

   Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Pou5f1 (Pou5f1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV U6-sgRNA UbC-GFP

    Plasmid

    #106948

    Purpose

    sgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP marker

    Depositor

    Albert Edge

    Article

    Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

    Insert

    sgRNA BsmBI/Esp3I cloning site
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX459-sgRNA2-RPB1-N-term

    Plasmid

    #195111

    Purpose

    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

    Depositor

    Job Dekker

    Article

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Insert

    sgRNA against first exon of RPB1 (N-terminal)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSB700 lenti gRNA iRFP713_zhang2.0

    Plasmid

    #167916

    Purpose

    Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. sgRNA1_Tet-inducible Luciferase reporter

    Plasmid

    #64161

    Purpose

    Photoactivatable transcription system. Mammalian expression of sgRNA1 to target Tet-inducible-luciferase reporter.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1 for Tet-inducible Luciferase reporter
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiCRISPR v2 puro hGSDME gRNA2

    Plasmid

    #223520

    Purpose

    Knocking out hGSDME in human cells

    Depositor

    Judy Lieberman

    Article

    Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

    Insert

    GSDME (GSDME Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-Bmpr2ΔKinase gRNA resistant

    Plasmid

    #163411

    Purpose

    Expresses BMPR-2ΔKinase resistant to the gRNAs(#163388-90) in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Expression

    Mammalian

    Mutation

    The kinase domain (202-500 aa) is deleted. gRNA-t…

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9(BB)-2A-tomato-TSC2-sgRNA

    Plasmid

    #196195

    Purpose

    Editing human TSC2 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    TSC2 (TSC2 Human)
    

    Use

    CRISPR

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV2-U6gRNA5(Ptpn23-g1)-PGKpuroBFP-W

    Plasmid

    #105034

    Purpose

    Lentiviral gRNA plasmid targeting mouse Ptpn23 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Ptpn23 (Ptpn23 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W

    Plasmid

    #67983

    Purpose

    Cas9 activity reporter (control) with GFP and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. lenti-sgRNA(MS2)_zeo_hCYP3A4_enhancer_guide1

    Plasmid

    #176839

    Purpose

    To recruit SAM complex to human CYP3A4 proximal enhancer.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

    Insert

    Human CYP3A4 proximal enhancer sgRNA for activation
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAN-PBAD-sgRNA-A2NT

    Plasmid

    #62257

    Purpose

    expression of A2NT sgRNA from the arabinose-inducible promoter

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    A2NT sgRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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