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LENTICRISPR-SNRNP70_sgRNA2

Plasmid

#201621

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

SNRNP70 (SNRNP70 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-NDUFB5_sgRNA1

Plasmid

#201606

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

NDUFB5 (NDUFB5 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-NDUFB5_sgRNA2

Plasmid

#201607

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

NDUFB5 (NDUFB5 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET32a-hNRG1a

Plasmid

#199235

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Mol Biol Rep. 2022 Dec;49(12):11715-1172

Insert

Neuregulin (NRG1 Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2zmiRFP-EF1-2xCMV-EF1-mClover3

Plasmid

#196112

Purpose

mammalian/zebrafish expression of zmiRFP-EF1-2xCMV-EF1-mClover3

Depositor

Reinhard Köster

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

zmiRFP-EF1-2xCMV-EF1-mClover3

Expression

Mammalian

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2zmiRFP2-EF1-2xCMV-EF1-mClover3

Plasmid

#196111

Purpose

mammalian/zebrafish expression of zmiRFP2-EF1-2xCMV-EF1-mClover3

Depositor

Reinhard Köster

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

zmiRFP2-EF1-2xCMV-EF1-mClover3

Expression

Mammalian

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2zTagRFP658-EF1-2xCMV-EF1-mClover3

Plasmid

#196109

Purpose

mammalian/zebrafish expression of zTagRFP658-EF1-2xCMV-EF1-mClover3

Depositor

Reinhard Köster

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

zTagRFP658-EF1-2xCMV-EF1-mClover3

Expression

Mammalian

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2zemiRFP2-EF1-2xCMV-EF1-mClover3

Plasmid

#196110

Purpose

mammalian/zebrafish expression of zemiRFP2-EF1-2xCMV-EF1-mClover3

Depositor

Reinhard Köster

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

zemiRFP2-EF1-2xCMV-EF1-mClover3

Expression

Mammalian

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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HH13 Clone 13 heavy chain

Plasmid

#192177

Purpose

Clone 13 heavy chain (HH013)

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 13 heavy chain (HH013)

Expression

Mammalian

Mutation

NA

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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HH13A Clone 13A heavy chain

Plasmid

#192178

Purpose

Clone 13A heavy chain (HH13A)

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 13A heavy chain (HH13A)

Expression

Mammalian

Mutation

NA

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_eGFP

Plasmid

#180379

Purpose

lentiviral transduction of eGFP gene

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

eGFP (eGFP )

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hSTK16-Flag

Plasmid

#180403

Purpose

lentiviral transduction of human STK16 (with a C-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

STK16 (STK16 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_Flag-hSRPK3

Plasmid

#180404

Purpose

lentiviral transduction of human SRPK3 (with an N-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

SRPK3 (SRPK3 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shTgm5-1

Plasmid

#180394

Purpose

Producing AAV that encodes mouse Tgm5 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shTgm5-1 (Tgm5 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shTgm5-2

Plasmid

#180395

Purpose

Producing AAV that encodes mouse Tgm5 shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shTgm5-2 (Tgm5 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shTgm5-3

Plasmid

#180396

Purpose

Producing AAV that encodes mouse Tgm5 shRNA-3 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shTgm5-3 (Tgm5 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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HK13A Clone 13A light chain

Plasmid

#192182

Purpose

Clone 13A light chain (HK13A)

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 13A light chain (HK13A)

Expression

Mammalian

Mutation

NA

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pKeratin-emiRFP2-N1

Plasmid

#178972

Purpose

Expresses the fusion protein of Keratin-emiRFP2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

Keratin-emiRFP2

Expression

Mammalian

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-miRFP2-IRES-HO1-N1

Plasmid

#178967

Purpose

Expresses miRFP2 and HO1 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

miRFP2

Tags

HO1 and no

Expression

Mammalian

Mutation

NO

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pFUGW-shRIIα

Plasmid

#183454

Purpose

sgRNA targeting rat PKA-RIIα subunits

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

sgRNA targeting rat PKA-RIIα (Prkar2a Rat)

Use

Lentiviral

Promoter

shRNA: H1 / gene: ubiquitin

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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