We narrowed to 7,168 results for: tet

1. pUt-mNF-GFP

   Plasmid

   #199220

   Purpose

   LPUtopia-7 matching RMCE donor plasmid with GFP Negative-Feedback circuit (mNF-GFP). Use BlastR for positive selection and HSV-TK for negative selection. 

   Depositor

   Gabor Balazsi

   Article

   Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

   Insert

   hTetR::P2A::EGFP
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV D2ir promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMAL c2x Tral FL-15D

   Plasmid

   #113010

   Purpose

   For bacterial expression of MBP fusion of full-length Drosophila Tral with Phos-mimic mutations

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   full length tral (tral Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   15 PNG phosphorylation sites mutated to D

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. E1b-GFP-Tol2-Gateway dre SE-irf2bpl C

   Plasmid

   #89372

   Purpose

   Vector for reporter assay of one zebrafish super-enhancer region

   Depositor

   Alena Shkumatava

   Article

   Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

   Insert

   irf2bpl (irf2bpl Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. E1b-GFP-Tol2-Gateway dre SE-irf2bpl B

   Plasmid

   #89371

   Purpose

   Vector for reporter assay of one zebrafish super-enhancer region

   Depositor

   Alena Shkumatava

   Article

   Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

   Insert

   irf2bpl (irf2bpl Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. E1b-GFP-Tol2-Gateway dre SE-irf2bpl D

   Plasmid

   #89373

   Purpose

   Vector for reporter assay of one zebrafish super-enhancer region

   Depositor

   Alena Shkumatava

   Article

   Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

   Insert

   irf2bpl (irf2bpl Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. E1b-GFP-Tol2-Gateway dre SE-irf2bpl A

   Plasmid

   #89370

   Purpose

   Vector for reporter assay of one zebrafish super-enhancer region

   Depositor

   Alena Shkumatava

   Article

   Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

   Insert

   irf2bpl (irf2bpl Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. RalA (G23V)(mature peptide)-pcw107-V5

   Plasmid

   #64643

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   RALA (RALA Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   G23V

   Promoter

   PGK

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

8. RalA (G23V)(mature peptide)-pcw107

   Plasmid

   #64642

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   RALA (RALA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   G23V

   Promoter

   PGK

   Available since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pinducer 20 DN-KASHΔPPPL

   Plasmid

   #129280

   Purpose

   Tet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPL

   Depositor

   Daniel Conway

   Article

   Zhang et al Curr Biol. 2019 Aug 2. pii: S0960-9822(1

   Insert

   SYNE1 (SYNE1 Human)
   

   Use

   Lentiviral

   Tags

   mcherry

   Expression

   Mutation

   the last 4 amino acids (PPPL) of the KASH domain …

   Promoter

   CMV

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-TRE3G-hYAP1-CMV-mCherry-T2A-Puro

    Plasmid

    #176850

    Purpose

    lentiviral vector for doxycycline-inducible YAP1 overexpression

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    hYAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    full length YAP1

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.puro_shGLS

    Plasmid

    #110335

    Purpose

    shGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene, puromycin selection

    Depositor

    Sandra Martha Gomes Dias

    Article

    Dias et al Oncogene. 2020 Jan;39(3):690-702. doi: 1

    Insert

    GLS glutaminase (GLS Synthetic, Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (RNA PolIII)

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. JAK2 (V617F)-pcw107-V5

    Plasmid

    #64610

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    JAK2 (JAK2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    V617F

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCW-ND-Cdt1ΔR198A/R210A-HA-Puro

    Plasmid

    #193769

    Purpose

    Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding (R198A/R210A), expressed from all-in-one pCW vector (TetOn + rtTA)

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    ND-Cdt1ΔR198A/R210A (CDT1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

    Promoter

    TRE

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFPC1-hVAP-A KD/MD

    Plasmid

    #104449

    Purpose

    Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein A (VAPA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    changed K94 to D and M96 to D

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV pCAG-FLEX2-tTA2-WPRE-bGHpA

    Plasmid

    #65458

    Purpose

    Can be used to generate AAV virus that will express the tetracycline transactivator tTA2 from the CAG promoter in a Cre-dependent manner

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    tTA2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFPC1-hVAP-B KD/MD

    Plasmid

    #104450

    Purpose

    Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein B (VAPB Human)
    

    Use

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    changed K87 to D and M89 to D

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCW57-Cx26-IRES-GCaMP6s

    Plasmid

    #188236

    Purpose

    3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 26 and GCaMP6s

    Depositor

    Fabio Mammano

    Article

    Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

    Insert

    GJB2 (GJB2 Human)
    

    Use

    Lentiviral

    Tags

    GCaMP6s

    Expression

    Mammalian

    Mutation

    Promoter

    Tight TRE promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Notch3 intracellular domain-pcw107-V5

    Plasmid

    #64623

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    NOTCH3 (NOTCH3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    intracellular domain *

    Promoter

    PGK

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pSPL3-hRPH3A_c.444Gwt

    Plasmid

    #190476

    Purpose

    minigene assay

    Depositor

    Alfredo Brusco

    Article

    Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

    Insert

    RPH3A (NM_014954.3) c.444 [exon 7 and part of surrounding introns only] (RPH3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCW-GemininΔDbox-HA-Puro

    Plasmid

    #193770

    Purpose

    Doxycyline-inducible (TetOn) human Geminin (C-terminal HA tag) with Dbox removed to prevent degradation by APC/C, expressed from all-in-one pCW vector (TetOn + rtTA)

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    GemininΔDbox-HA (GMNN Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Dbox removed (R23A, L26A). Codons switched at siR…

    Promoter

    TRE

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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